Bacillus phage Moonbeam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Moonbeamvirus; Bacillus virus Moonbeam

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RP94|A0A0A0RP94_9CAUD Pectate_lyase_3 domain-containing protein OS=Bacillus phage Moonbeam OX=1540091 GN=CPT_Moonbeam51 PE=4 SV=1
MM1 pKa = 7.48FGFTEE6 pKa = 4.96HH7 pKa = 6.04EE8 pKa = 3.88MEE10 pKa = 4.4MYY12 pKa = 10.41QDD14 pKa = 3.52AVQAFAIEE22 pKa = 4.15EE23 pKa = 4.19VATNIVEE30 pKa = 4.07NSKK33 pKa = 10.46FVAPATEE40 pKa = 4.02QNGKK44 pKa = 9.54IVVSDD49 pKa = 3.81RR50 pKa = 11.84FVQGVFNDD58 pKa = 3.56VQTVITITAAAMAYY72 pKa = 9.59EE73 pKa = 4.21YY74 pKa = 11.43AFLQDD79 pKa = 4.26DD80 pKa = 4.52GEE82 pKa = 4.55TTEE85 pKa = 4.72EE86 pKa = 3.64DD87 pKa = 4.35TIAHH91 pKa = 6.99LHH93 pKa = 5.46TKK95 pKa = 10.13YY96 pKa = 10.13EE97 pKa = 4.06SHH99 pKa = 8.12LIRR102 pKa = 11.84QFINYY107 pKa = 9.67SIVFTEE113 pKa = 4.57DD114 pKa = 3.12VLPVIVSEE122 pKa = 4.94LVMEE126 pKa = 4.46MPYY129 pKa = 10.64LYY131 pKa = 10.71AAARR135 pKa = 11.84SSDD138 pKa = 3.57EE139 pKa = 4.12FDD141 pKa = 5.25DD142 pKa = 4.22EE143 pKa = 5.1LFLEE147 pKa = 4.62EE148 pKa = 4.98RR149 pKa = 11.84LAAYY153 pKa = 8.71TEE155 pKa = 4.24YY156 pKa = 10.96LDD158 pKa = 4.1SNFYY162 pKa = 10.48EE163 pKa = 4.33YY164 pKa = 11.03SGLDD168 pKa = 3.38DD169 pKa = 5.54YY170 pKa = 12.08EE171 pKa = 6.2DD172 pKa = 4.23FDD174 pKa = 4.85EE175 pKa = 6.94DD176 pKa = 3.67EE177 pKa = 5.34AEE179 pKa = 4.18LNEE182 pKa = 5.44DD183 pKa = 4.99DD184 pKa = 5.37FDD186 pKa = 4.08EE187 pKa = 4.63

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RN74|A0A0A0RN74_9CAUD DNA recombination and repair protein OS=Bacillus phage Moonbeam OX=1540091 GN=CPT_Moonbeam127 PE=3 SV=1
MM1 pKa = 7.43FKK3 pKa = 10.65FIVYY7 pKa = 10.04LAFCVASGIGIGQALSTYY25 pKa = 10.62LKK27 pKa = 9.71LTGSIHH33 pKa = 6.61RR34 pKa = 11.84SVVALAFIACGIFATSLQRR53 pKa = 11.84IFYY56 pKa = 9.69GGKK59 pKa = 9.17RR60 pKa = 3.4

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

231

0

231

47869

27

1301

207.2

23.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.491 ± 0.168

0.815 ± 0.069

6.171 ± 0.119

7.629 ± 0.316

3.877 ± 0.088

6.631 ± 0.258

1.801 ± 0.091

6.286 ± 0.152

7.767 ± 0.242

8.195 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.098

5.415 ± 0.146

3.315 ± 0.154

3.731 ± 0.13

4.341 ± 0.133

5.872 ± 0.17

6.672 ± 0.242

6.927 ± 0.18

1.049 ± 0.056

4.466 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski