Avian paramyxovirus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Avulavirinae; Paraavulavirus; Avian paraavulavirus 4

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G5D6F8|G5D6F8_9MONO Matrix protein OS=Avian paramyxovirus 4 OX=28274 GN=M PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.28FTDD5 pKa = 3.49IDD7 pKa = 3.99AVNSLIEE14 pKa = 4.2SSSAIIDD21 pKa = 4.24SIQHH25 pKa = 6.27GGLQPSGTVGLSQIPKK41 pKa = 9.85GITSALTKK49 pKa = 10.3AWEE52 pKa = 4.33AEE54 pKa = 3.8AATAGNGDD62 pKa = 4.04TQHH65 pKa = 6.92KK66 pKa = 9.11PDD68 pKa = 4.52SPEE71 pKa = 3.61DD72 pKa = 3.57HH73 pKa = 6.59QANDD77 pKa = 3.54TDD79 pKa = 4.21SPEE82 pKa = 4.12DD83 pKa = 3.16TGTNQTIQEE92 pKa = 4.19ANIVEE97 pKa = 4.59TPHH100 pKa = 7.17PEE102 pKa = 3.86VLSAAKK108 pKa = 10.28ARR110 pKa = 11.84LKK112 pKa = 10.52RR113 pKa = 11.84PKK115 pKa = 10.13AGRR118 pKa = 11.84DD119 pKa = 3.15THH121 pKa = 7.16DD122 pKa = 4.34NPSAQPDD129 pKa = 3.72HH130 pKa = 6.39FLKK133 pKa = 10.8GGPLSPQPAAPWVQSPPIHH152 pKa = 6.48GGPGTVDD159 pKa = 3.27PRR161 pKa = 11.84PSQTQDD167 pKa = 2.49HH168 pKa = 6.64SLTGEE173 pKa = 3.94KK174 pKa = 9.01WQSSPTKK181 pKa = 10.37QPEE184 pKa = 4.07TLNWWNGATRR194 pKa = 11.84GAPQSEE200 pKa = 4.45LNQPDD205 pKa = 3.62STVYY209 pKa = 11.23ADD211 pKa = 3.76TAPPSAASACMTTDD225 pKa = 3.51QVQLLMKK232 pKa = 9.77EE233 pKa = 4.25VADD236 pKa = 3.95MKK238 pKa = 11.11SLLQALVKK246 pKa = 10.54NLAVLPQLRR255 pKa = 11.84NEE257 pKa = 4.05VAAIRR262 pKa = 11.84TSQAMIEE269 pKa = 4.22GTLNSIKK276 pKa = 10.48ILDD279 pKa = 4.05PGNYY283 pKa = 9.02QEE285 pKa = 5.53SSLNSWFKK293 pKa = 10.31PRR295 pKa = 11.84QDD297 pKa = 3.2HH298 pKa = 5.93AVVVSGPGNPLTMPTPIQDD317 pKa = 3.28NTIFLDD323 pKa = 3.7EE324 pKa = 4.7LARR327 pKa = 11.84PHH329 pKa = 6.98PSLVNPSPPTTNTNVDD345 pKa = 4.43LGPQKK350 pKa = 10.25QAAIAYY356 pKa = 9.21ISAKK360 pKa = 10.48CKK362 pKa = 10.78DD363 pKa = 3.41PGKK366 pKa = 10.12RR367 pKa = 11.84DD368 pKa = 3.54QLSKK372 pKa = 10.72LIEE375 pKa = 4.23RR376 pKa = 11.84ATTLSEE382 pKa = 3.71INKK385 pKa = 9.41VKK387 pKa = 10.56RR388 pKa = 11.84QALGLL393 pKa = 3.93

Molecular weight:
42.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G5D6F9|G5D6F9_9MONO Fusion glycoprotein F0 OS=Avian paramyxovirus 4 OX=28274 GN=F PE=3 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.45MDD5 pKa = 4.25TVYY8 pKa = 10.71INLMADD14 pKa = 3.69DD15 pKa = 4.42PTHH18 pKa = 6.18QKK20 pKa = 10.6EE21 pKa = 4.43LLSFPLVPVTGPDD34 pKa = 3.39GKK36 pKa = 10.78KK37 pKa = 9.81EE38 pKa = 3.91LQHH41 pKa = 5.62QVRR44 pKa = 11.84TQSLLASDD52 pKa = 3.93KK53 pKa = 9.01QTEE56 pKa = 4.07RR57 pKa = 11.84FIFLNTYY64 pKa = 9.8GFIYY68 pKa = 9.16DD69 pKa = 3.76TTPDD73 pKa = 3.29KK74 pKa = 9.65TTFSTPEE81 pKa = 4.67HH82 pKa = 6.29INQPKK87 pKa = 8.47RR88 pKa = 11.84TMVSAAMMTIGLVPANIPLNEE109 pKa = 3.88LTATVFGLKK118 pKa = 10.05VRR120 pKa = 11.84VRR122 pKa = 11.84KK123 pKa = 8.75SARR126 pKa = 11.84YY127 pKa = 9.39RR128 pKa = 11.84EE129 pKa = 4.36VVWYY133 pKa = 9.53QCNPVPALLAATRR146 pKa = 11.84FGRR149 pKa = 11.84QGGLEE154 pKa = 4.13SSTGVSVKK162 pKa = 10.51APEE165 pKa = 5.52KK166 pKa = 10.09IDD168 pKa = 3.52CEE170 pKa = 3.95KK171 pKa = 11.01DD172 pKa = 3.21YY173 pKa = 11.09TYY175 pKa = 11.38YY176 pKa = 10.37PYY178 pKa = 10.5FLSVCYY184 pKa = 10.01IATSNLFKK192 pKa = 10.5VPKK195 pKa = 9.4MVANATNSQLYY206 pKa = 9.02HH207 pKa = 6.19LTMQVTFAFPKK218 pKa = 10.26NIPPANQKK226 pKa = 10.5LLTQVDD232 pKa = 3.83EE233 pKa = 4.71GFEE236 pKa = 4.29GTVDD240 pKa = 3.43CHH242 pKa = 6.74FGNMLKK248 pKa = 10.04KK249 pKa = 10.15DD250 pKa = 3.48RR251 pKa = 11.84KK252 pKa = 10.08GNMRR256 pKa = 11.84TLSQAADD263 pKa = 3.29KK264 pKa = 10.17VRR266 pKa = 11.84RR267 pKa = 11.84MNILVGIFDD276 pKa = 3.87LHH278 pKa = 6.63GPTLFLEE285 pKa = 4.56YY286 pKa = 9.93TGKK289 pKa = 8.51LTKK292 pKa = 10.53ALLGFMSTSRR302 pKa = 11.84TAIIPISQLNPMLGQLMWSSDD323 pKa = 3.25AQIVKK328 pKa = 10.1LRR330 pKa = 11.84VVITTSKK337 pKa = 10.43RR338 pKa = 11.84GPCGGEE344 pKa = 3.48QEE346 pKa = 4.66YY347 pKa = 11.4VLDD350 pKa = 4.31PKK352 pKa = 10.51FTVKK356 pKa = 10.42KK357 pKa = 9.94EE358 pKa = 3.9KK359 pKa = 10.68ARR361 pKa = 11.84LNPFKK366 pKa = 10.7KK367 pKa = 10.21AAQQ370 pKa = 3.49

Molecular weight:
41.49 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4562

370

2211

760.3

84.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.278 ± 0.849

1.797 ± 0.328

5.151 ± 0.235

4.472 ± 0.369

3.31 ± 0.393

5.612 ± 0.352

2.148 ± 0.419

6.094 ± 0.466

4.143 ± 0.616

11.201 ± 0.799

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.28

4.822 ± 0.35

5.217 ± 0.595

5.129 ± 0.421

5.261 ± 0.353

8.593 ± 0.628

6.664 ± 0.547

6.203 ± 0.399

1.162 ± 0.23

3.42 ± 0.425

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski