Bat Hp-betacoronavirus/Zhejiang2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Hibecovirus; Bat Hp-betacoronavirus Zhejiang2013

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088DI26|A0A088DI26_9BETC Nucleoprotein OS=Bat Hp-betacoronavirus/Zhejiang2013 OX=1541205 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.19FFSLYY7 pKa = 10.27TFGSFHH13 pKa = 5.26MHH15 pKa = 6.13AAKK18 pKa = 10.22LASLLSNSTEE28 pKa = 3.95HH29 pKa = 7.12VEE31 pKa = 4.89QAVQHH36 pKa = 6.5LNTKK40 pKa = 8.64LTADD44 pKa = 2.95IMLCIFLVIYY54 pKa = 7.4LICYY58 pKa = 8.01FLRR61 pKa = 11.84TDD63 pKa = 3.21SFIAVVFKK71 pKa = 10.85YY72 pKa = 10.42LAGLLTGGFLCLGLFLDD89 pKa = 4.96TPTLLLKK96 pKa = 10.03ATIGVVLFMFSLGFICRR113 pKa = 11.84ITLAIRR119 pKa = 11.84CKK121 pKa = 10.76SLVPLCADD129 pKa = 3.85DD130 pKa = 5.34DD131 pKa = 4.58CFVNYY136 pKa = 10.07NAGGKK141 pKa = 6.52TYY143 pKa = 10.82CMPFDD148 pKa = 4.5PNEE151 pKa = 4.25PYY153 pKa = 9.55LTLVVHH159 pKa = 6.4QNGITCGSYY168 pKa = 10.85KK169 pKa = 10.7LYY171 pKa = 10.91GDD173 pKa = 3.43VSIADD178 pKa = 4.61RR179 pKa = 11.84IYY181 pKa = 11.13LVTLTKK187 pKa = 10.32SVPYY191 pKa = 9.98SLQNIFDD198 pKa = 4.6AEE200 pKa = 4.3LCTIAFYY207 pKa = 10.48IADD210 pKa = 3.74CAVIEE215 pKa = 4.25DD216 pKa = 4.23HH217 pKa = 5.85TTAGKK222 pKa = 8.25TPRR225 pKa = 11.84LEE227 pKa = 4.47LKK229 pKa = 10.12SDD231 pKa = 4.79PIYY234 pKa = 10.19EE235 pKa = 4.47VPCATIDD242 pKa = 3.58VPLL245 pKa = 5.05

Molecular weight: 27.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088DIE1|A0A088DIE1_9BETC 2'-O-methyltransferase OS=Bat Hp-betacoronavirus/Zhejiang2013 OX=1541205 PE=3 SV=1
MM1 pKa = 7.31AAEE4 pKa = 4.32GQRR7 pKa = 11.84VTFLTQGSNQNNNNQEE23 pKa = 4.12NQDD26 pKa = 3.44GRR28 pKa = 11.84GARR31 pKa = 11.84PKK33 pKa = 9.17TRR35 pKa = 11.84KK36 pKa = 8.95PRR38 pKa = 11.84PAPQNNVSWFTPLTQHH54 pKa = 6.68GKK56 pKa = 9.18QALTFNPGQGVPLNANDD73 pKa = 5.2DD74 pKa = 3.75PDD76 pKa = 4.07SRR78 pKa = 11.84IGYY81 pKa = 5.38WRR83 pKa = 11.84RR84 pKa = 11.84NIRR87 pKa = 11.84TVTKK91 pKa = 10.3NGKK94 pKa = 6.92PQKK97 pKa = 10.34LDD99 pKa = 3.39PRR101 pKa = 11.84WYY103 pKa = 10.04FYY105 pKa = 11.53YY106 pKa = 10.65LGTGPEE112 pKa = 3.91QNLKK116 pKa = 10.49YY117 pKa = 10.28LQQKK121 pKa = 10.41DD122 pKa = 3.88GIVWVAAHH130 pKa = 6.71GALNVPVKK138 pKa = 10.02TVGTRR143 pKa = 11.84NPANDD148 pKa = 3.52QAIVAAFAPGTEE160 pKa = 4.22LPKK163 pKa = 10.83GFYY166 pKa = 10.61VEE168 pKa = 5.11GSRR171 pKa = 11.84STSSASSAASSRR183 pKa = 11.84SNSRR187 pKa = 11.84SRR189 pKa = 11.84NSSLSRR195 pKa = 11.84SSSPGRR201 pKa = 11.84GLPPATDD208 pKa = 3.94PNGVLAALLLTKK220 pKa = 10.73LEE222 pKa = 4.29ALDD225 pKa = 4.4AKK227 pKa = 11.27VNGPKK232 pKa = 9.84QPPVVTKK239 pKa = 9.39KK240 pKa = 8.76TAAEE244 pKa = 3.84IAAKK248 pKa = 9.83PRR250 pKa = 11.84QKK252 pKa = 10.25RR253 pKa = 11.84VAHH256 pKa = 6.45KK257 pKa = 10.33GYY259 pKa = 10.37NVNAAYY265 pKa = 10.1GRR267 pKa = 11.84RR268 pKa = 11.84GPGPYY273 pKa = 9.33QGNFGTQEE281 pKa = 3.91FNKK284 pKa = 10.55LGTDD288 pKa = 3.65YY289 pKa = 11.06PKK291 pKa = 10.28WPQIAQLAPTPSAFFGMSRR310 pKa = 11.84FAVQKK315 pKa = 10.75NDD317 pKa = 3.33DD318 pKa = 4.31GTWLTYY324 pKa = 10.12HH325 pKa = 6.3GHH327 pKa = 6.05IKK329 pKa = 9.7MDD331 pKa = 3.35EE332 pKa = 4.13SDD334 pKa = 4.11PNFQVWMTEE343 pKa = 3.81LQQNIDD349 pKa = 3.2AYY351 pKa = 10.32KK352 pKa = 10.5NFPQKK357 pKa = 10.37EE358 pKa = 4.35EE359 pKa = 4.06KK360 pKa = 10.17KK361 pKa = 10.23SRR363 pKa = 11.84KK364 pKa = 9.22PKK366 pKa = 9.07TKK368 pKa = 10.19NVDD371 pKa = 3.33MAPQDD376 pKa = 3.68VMGAAAVDD384 pKa = 4.3LEE386 pKa = 4.49WDD388 pKa = 3.62SSIDD392 pKa = 3.35QTGPNTIVVKK402 pKa = 10.14PKK404 pKa = 10.15KK405 pKa = 8.97QRR407 pKa = 11.84KK408 pKa = 7.3PAADD412 pKa = 3.59NNISEE417 pKa = 4.39II418 pKa = 3.96

Molecular weight: 45.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

10159

72

7247

1128.8

126.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.097 ± 0.29

3.406 ± 0.444

5.375 ± 0.609

4.233 ± 0.376

4.922 ± 0.318

5.571 ± 0.288

2.136 ± 0.237

4.991 ± 0.409

5.512 ± 0.758

8.406 ± 0.588

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.229

5.473 ± 0.628

4.213 ± 0.606

3.642 ± 0.686

3.691 ± 0.323

6.566 ± 0.298

7.668 ± 0.951

8.859 ± 0.748

1.073 ± 0.165

4.676 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski