Streptomyces aidingensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1HLM9|A0A1I1HLM9_9ACTN Uncharacterized protein OS=Streptomyces aidingensis OX=910347 GN=SAMN05421773_102426 PE=4 SV=1
MM1 pKa = 7.35SNWRR5 pKa = 11.84KK6 pKa = 9.82ISVSMAGLAVLVTTAACGNANAGNDD31 pKa = 4.01DD32 pKa = 4.57PYY34 pKa = 11.57ADD36 pKa = 3.86PAALSEE42 pKa = 4.06EE43 pKa = 4.17DD44 pKa = 3.05SGYY47 pKa = 11.11GYY49 pKa = 10.75DD50 pKa = 4.01YY51 pKa = 11.16GDD53 pKa = 3.72EE54 pKa = 4.49GGEE57 pKa = 3.9QAQGAEE63 pKa = 4.28GAEE66 pKa = 4.29GAVQLAAAGEE76 pKa = 4.16LATYY80 pKa = 10.68DD81 pKa = 3.52SGEE84 pKa = 4.08LGEE87 pKa = 4.37LVKK90 pKa = 11.04DD91 pKa = 3.87GEE93 pKa = 4.69GFTLYY98 pKa = 10.72RR99 pKa = 11.84FDD101 pKa = 5.12EE102 pKa = 4.99DD103 pKa = 4.27SADD106 pKa = 3.81PPASNCAGDD115 pKa = 4.89CAVNWPPVSADD126 pKa = 3.08AAAGTGIDD134 pKa = 3.93PGLLGTVRR142 pKa = 11.84RR143 pKa = 11.84ADD145 pKa = 3.26GSEE148 pKa = 3.68QLTIAGWPAYY158 pKa = 9.72RR159 pKa = 11.84YY160 pKa = 10.51AGDD163 pKa = 3.57TGPEE167 pKa = 3.69QTAGQGAGGSWWAIAADD184 pKa = 4.13GARR187 pKa = 11.84ASGQGGAEE195 pKa = 4.25GAGEE199 pKa = 4.07AAGDD203 pKa = 3.89LPGLSVFEE211 pKa = 4.1HH212 pKa = 6.92PEE214 pKa = 3.6YY215 pKa = 11.43GEE217 pKa = 4.0ILRR220 pKa = 11.84DD221 pKa = 3.63GQGRR225 pKa = 11.84TLYY228 pKa = 10.66RR229 pKa = 11.84FADD232 pKa = 4.0DD233 pKa = 4.18SAWPMEE239 pKa = 4.32SLCTGDD245 pKa = 6.19CLDD248 pKa = 3.87NWPAAGPPADD258 pKa = 4.0PADD261 pKa = 3.85VAGVDD266 pKa = 4.25AEE268 pKa = 4.36LLDD271 pKa = 3.94VFNRR275 pKa = 11.84PDD277 pKa = 4.87GIQQLVIDD285 pKa = 4.64CWPLYY290 pKa = 10.18RR291 pKa = 11.84YY292 pKa = 10.39AGDD295 pKa = 4.23AEE297 pKa = 4.56PGDD300 pKa = 3.96VNGQGVGGKK309 pKa = 8.57WYY311 pKa = 10.54VVSPEE316 pKa = 3.59GKK318 pKa = 9.23MIKK321 pKa = 9.98SS322 pKa = 3.53

Molecular weight: 33.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1QPI5|A0A1I1QPI5_9ACTN Pyruvate dehydrogenase E1 component alpha subunit OS=Streptomyces aidingensis OX=910347 GN=SAMN05421773_111216 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.42GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5601

0

5601

1894568

30

5052

338.3

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.213 ± 0.054

0.838 ± 0.011

5.748 ± 0.025

6.089 ± 0.038

2.531 ± 0.017

10.027 ± 0.04

2.361 ± 0.015

2.92 ± 0.022

1.512 ± 0.018

10.427 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.668 ± 0.013

1.584 ± 0.017

6.849 ± 0.035

2.638 ± 0.019

8.983 ± 0.043

4.584 ± 0.022

5.89 ± 0.036

7.671 ± 0.032

1.532 ± 0.014

1.935 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski