Pseudomonas xinjiangensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1XY70|A0A1H1XY70_9PSED Tryptophan synthase beta chain OS=Pseudomonas xinjiangensis OX=487184 GN=trpB PE=3 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84IIRR5 pKa = 11.84FILPLLFSSLASAAPTEE22 pKa = 4.35IDD24 pKa = 3.54WLDD27 pKa = 3.85LLPQEE32 pKa = 5.81DD33 pKa = 4.61YY34 pKa = 11.41QAMLDD39 pKa = 3.73MPEE42 pKa = 4.66IGHH45 pKa = 7.24DD46 pKa = 4.1SGDD49 pKa = 3.63EE50 pKa = 4.18TPGDD54 pKa = 3.67FTAGMRR60 pKa = 11.84QRR62 pKa = 11.84DD63 pKa = 3.79EE64 pKa = 4.33SLPDD68 pKa = 3.02VMYY71 pKa = 10.58SRR73 pKa = 11.84RR74 pKa = 11.84VVDD77 pKa = 4.38SYY79 pKa = 11.76DD80 pKa = 3.46GQAVQIGGYY89 pKa = 8.4PVPLEE94 pKa = 4.01TSEE97 pKa = 4.05TGAYY101 pKa = 9.02TLFFLVPYY109 pKa = 10.12AGACIHH115 pKa = 6.08VPPPPPNQIILVEE128 pKa = 4.27YY129 pKa = 9.96ADD131 pKa = 4.15GMRR134 pKa = 11.84IDD136 pKa = 5.6DD137 pKa = 4.15IYY139 pKa = 11.36VPLWVSGTLQVDD151 pKa = 3.47QTSNEE156 pKa = 4.06LADD159 pKa = 3.44ASYY162 pKa = 10.79RR163 pKa = 11.84IVAQRR168 pKa = 11.84VWPYY172 pKa = 11.1DD173 pKa = 3.4GEE175 pKa = 4.27

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1PCM8|A0A1H1PCM8_9PSED Solute carrier family 34 (Sodium-dependent phosphate cotransporter) OS=Pseudomonas xinjiangensis OX=487184 GN=SAMN05216421_0885 PE=4 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84RR4 pKa = 11.84FHH6 pKa = 7.08RR7 pKa = 11.84LSTWYY12 pKa = 9.17PSQGKK17 pKa = 9.53VSNQLGNDD25 pKa = 3.24EE26 pKa = 4.76TLTVLYY32 pKa = 10.78DD33 pKa = 3.89GGCPLCMRR41 pKa = 11.84EE42 pKa = 3.77IAHH45 pKa = 5.78VKK47 pKa = 9.75GLAEE51 pKa = 4.0RR52 pKa = 11.84RR53 pKa = 11.84EE54 pKa = 3.96DD55 pKa = 3.41SALRR59 pKa = 11.84FVDD62 pKa = 3.78ISSGASDD69 pKa = 3.94CAAFADD75 pKa = 4.93DD76 pKa = 5.18RR77 pKa = 11.84EE78 pKa = 4.17KK79 pKa = 11.21LLARR83 pKa = 11.84FHH85 pKa = 5.63VQRR88 pKa = 11.84SDD90 pKa = 2.91GSRR93 pKa = 11.84LDD95 pKa = 3.38GAEE98 pKa = 4.2AFVAMWSRR106 pKa = 11.84LPGWRR111 pKa = 11.84WLARR115 pKa = 11.84VARR118 pKa = 11.84LPGGLALFEE127 pKa = 4.06LSYY130 pKa = 11.06RR131 pKa = 11.84GFLRR135 pKa = 11.84VRR137 pKa = 11.84PALQRR142 pKa = 11.84LARR145 pKa = 11.84QAEE148 pKa = 4.32AKK150 pKa = 9.78RR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 3.6

Molecular weight: 17.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3245

0

3245

1066341

29

2431

328.6

36.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.693 ± 0.047

0.977 ± 0.012

5.694 ± 0.032

6.257 ± 0.04

3.537 ± 0.029

7.991 ± 0.038

2.23 ± 0.02

4.925 ± 0.03

2.998 ± 0.034

11.283 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.02

2.865 ± 0.025

4.846 ± 0.027

4.365 ± 0.032

6.933 ± 0.042

5.782 ± 0.034

4.912 ± 0.027

7.269 ± 0.034

1.423 ± 0.018

2.477 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski