Clostridiaceae bacterium 14S0207

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; unclassified Clostridiaceae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2714 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4WF69|A0A2Z4WF69_9CLOT ABC transporter domain-containing protein OS=Clostridiaceae bacterium 14S0207 OX=2082193 GN=C3495_14245 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 4.86YY3 pKa = 9.26MPEE6 pKa = 4.25VIQVIPTDD14 pKa = 3.58DD15 pKa = 3.79FKK17 pKa = 11.79VYY19 pKa = 10.83VYY21 pKa = 10.73FDD23 pKa = 4.3DD24 pKa = 5.97GSIHH28 pKa = 7.31LYY30 pKa = 9.76DD31 pKa = 4.23AKK33 pKa = 10.95EE34 pKa = 4.46LILKK38 pKa = 9.95GIFKK42 pKa = 10.58VLQDD46 pKa = 3.26INIFKK51 pKa = 10.13EE52 pKa = 3.7KK53 pKa = 9.9CTVLNGTLAWSLDD66 pKa = 3.42DD67 pKa = 5.64SYY69 pKa = 12.22DD70 pKa = 3.39EE71 pKa = 4.48STCLDD76 pKa = 3.15IDD78 pKa = 3.74PFVIYY83 pKa = 10.15EE84 pKa = 4.45DD85 pKa = 3.94YY86 pKa = 11.25PEE88 pKa = 4.21VSEE91 pKa = 4.91PMEE94 pKa = 3.96LFKK97 pKa = 11.43NNKK100 pKa = 9.5DD101 pKa = 3.51EE102 pKa = 4.5YY103 pKa = 11.42NCNTDD108 pKa = 3.53KK109 pKa = 10.87NTGNN113 pKa = 3.67

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4WDA1|A0A2Z4WDA1_9CLOT Flagellar biosynthetic protein FliR OS=Clostridiaceae bacterium 14S0207 OX=2082193 GN=C3495_04985 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.89KK9 pKa = 7.86RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 9.9RR21 pKa = 11.84MNSASGRR28 pKa = 11.84NVLKK32 pKa = 10.35RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.91GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2714

0

2714

856979

23

3033

315.8

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.963 ± 0.046

1.311 ± 0.022

5.154 ± 0.034

7.498 ± 0.063

4.49 ± 0.042

6.088 ± 0.045

1.321 ± 0.019

10.23 ± 0.068

10.059 ± 0.066

9.234 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.025

6.739 ± 0.052

2.701 ± 0.03

2.506 ± 0.027

3.175 ± 0.029

6.128 ± 0.046

4.862 ± 0.044

6.253 ± 0.045

0.647 ± 0.012

4.112 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski