Klebsiella phage KMI5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N7V1|A0A5B9N7V1_9CAUD Uncharacterized protein OS=Klebsiella phage KMI5 OX=2601616 GN=KMI5_35 PE=4 SV=1
MM1 pKa = 7.75IKK3 pKa = 10.43PGDD6 pKa = 3.73IVEE9 pKa = 4.16MLEE12 pKa = 5.24LGPEE16 pKa = 4.42PIGPEE21 pKa = 3.66LSSYY25 pKa = 9.24FVPGSQHH32 pKa = 6.5RR33 pKa = 11.84VISYY37 pKa = 10.58CEE39 pKa = 3.92EE40 pKa = 3.7TWEE43 pKa = 4.73VEE45 pKa = 5.53LIDD48 pKa = 3.81PTNGSEE54 pKa = 4.2EE55 pKa = 4.37PGDD58 pKa = 4.17GITFFPGEE66 pKa = 3.93YY67 pKa = 10.18KK68 pKa = 10.88LIVEE72 pKa = 4.84

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N7S4|A0A5B9N7S4_9CAUD Uncharacterized protein OS=Klebsiella phage KMI5 OX=2601616 GN=KMI5_5 PE=4 SV=1
MM1 pKa = 7.77AEE3 pKa = 4.03NNRR6 pKa = 11.84ATVVYY11 pKa = 10.5DD12 pKa = 3.41EE13 pKa = 4.97TSPSGLRR20 pKa = 11.84YY21 pKa = 6.72TASRR25 pKa = 11.84FKK27 pKa = 11.14SRR29 pKa = 11.84IGAPAGALNSKK40 pKa = 9.98GYY42 pKa = 10.5YY43 pKa = 9.6DD44 pKa = 5.02LRR46 pKa = 11.84SAPSGCNSRR55 pKa = 11.84MAHH58 pKa = 4.77AVVWEE63 pKa = 4.08LHH65 pKa = 6.34NGDD68 pKa = 4.31IPDD71 pKa = 4.01GLEE74 pKa = 3.58VDD76 pKa = 5.23HH77 pKa = 7.81IDD79 pKa = 4.61GNPSNNRR86 pKa = 11.84IEE88 pKa = 4.27NLRR91 pKa = 11.84LATRR95 pKa = 11.84ALQAFNRR102 pKa = 11.84GVRR105 pKa = 11.84TDD107 pKa = 3.46KK108 pKa = 11.06RR109 pKa = 11.84SGLPKK114 pKa = 10.41GVFVNGAGFMGRR126 pKa = 11.84TSRR129 pKa = 11.84DD130 pKa = 2.72GVRR133 pKa = 11.84ISKK136 pKa = 8.82TFDD139 pKa = 3.65NIEE142 pKa = 3.8EE143 pKa = 3.93AAIWVAEE150 pKa = 3.93ARR152 pKa = 11.84RR153 pKa = 11.84VSARR157 pKa = 11.84AWVRR161 pKa = 11.84SS162 pKa = 3.63

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13557

65

1232

271.1

29.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.238 ± 0.561

1.328 ± 0.243

6.13 ± 0.238

5.746 ± 0.317

3.032 ± 0.149

7.561 ± 0.229

1.822 ± 0.176

4.529 ± 0.199

4.632 ± 0.288

8.542 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.205

4.168 ± 0.23

3.828 ± 0.244

4.927 ± 0.347

5.997 ± 0.259

6.38 ± 0.261

5.857 ± 0.257

7.096 ± 0.257

1.424 ± 0.129

3.828 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski