Acidaminobacter hydrogenoformans DSM 2784

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XII. Incertae Sedis; Acidaminobacter; Acidaminobacter hydrogenoformans

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5RQP3|A0A1G5RQP3_9FIRM Aminobenzoyl-glutamate transport protein OS=Acidaminobacter hydrogenoformans DSM 2784 OX=1120920 GN=SAMN03080599_00228 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 10.23RR3 pKa = 11.84SSRR6 pKa = 11.84FLAISLSLMLVFQAFVIAPISWAADD31 pKa = 3.23FDD33 pKa = 3.9ITLDD37 pKa = 3.74GSQSATVNNAIFKK50 pKa = 9.91QSPSDD55 pKa = 3.46SSTGTGIFTPFVRR68 pKa = 11.84VQGNGTEE75 pKa = 4.07SGYY78 pKa = 8.73NTDD81 pKa = 3.29GTTEE85 pKa = 4.21FDD87 pKa = 3.51TKK89 pKa = 9.45TGKK92 pKa = 6.86WTHH95 pKa = 6.69AIKK98 pKa = 10.76LSDD101 pKa = 3.62IPTVDD106 pKa = 3.41VQGKK110 pKa = 8.32FYY112 pKa = 11.16RR113 pKa = 11.84EE114 pKa = 3.75LLLDD118 pKa = 4.14LNEE121 pKa = 4.63TKK123 pKa = 10.56NDD125 pKa = 3.69PYY127 pKa = 10.83IQLDD131 pKa = 3.72EE132 pKa = 4.56FEE134 pKa = 4.35IWLTKK139 pKa = 10.86DD140 pKa = 3.35KK141 pKa = 10.84DD142 pKa = 3.25ISGYY146 pKa = 10.11PFTTDD151 pKa = 2.81QAIMVFSLEE160 pKa = 4.23GEE162 pKa = 4.56SILLDD167 pKa = 3.42YY168 pKa = 10.58RR169 pKa = 11.84INSGSGWGDD178 pKa = 3.4YY179 pKa = 10.14KK180 pKa = 11.44ALIPDD185 pKa = 4.0EE186 pKa = 5.15LFAGYY191 pKa = 10.89GNDD194 pKa = 3.28YY195 pKa = 11.01YY196 pKa = 11.13FVLFSKK202 pKa = 9.19LTQADD207 pKa = 3.59SGFEE211 pKa = 3.79EE212 pKa = 4.49WGVLSSDD219 pKa = 2.61ITAAPSVDD227 pKa = 3.15IEE229 pKa = 4.23KK230 pKa = 10.63LISIDD235 pKa = 5.84DD236 pKa = 4.08GEE238 pKa = 4.52TWLDD242 pKa = 3.28ADD244 pKa = 3.96TSEE247 pKa = 4.22DD248 pKa = 3.5TVLAVYY254 pKa = 9.94PGTVHH259 pKa = 6.68FKK261 pKa = 11.02VIIEE265 pKa = 4.14NTGNVPLTNVAVTDD279 pKa = 4.39FSDD282 pKa = 3.81DD283 pKa = 3.55SEE285 pKa = 6.77AIDD288 pKa = 3.73FTGLNDD294 pKa = 3.65YY295 pKa = 10.95LGLGDD300 pKa = 5.46KK301 pKa = 10.59IEE303 pKa = 4.48SDD305 pKa = 4.38PIEE308 pKa = 4.18VSTILRR314 pKa = 11.84SHH316 pKa = 6.99ANTATINAYY325 pKa = 9.52YY326 pKa = 10.32GKK328 pKa = 10.49EE329 pKa = 3.9KK330 pKa = 10.33VTDD333 pKa = 3.65SDD335 pKa = 3.73SANYY339 pKa = 7.52YY340 pKa = 7.74TLPDD344 pKa = 3.79PKK346 pKa = 10.36IAINKK351 pKa = 5.86TTNGDD356 pKa = 3.63DD357 pKa = 3.94DD358 pKa = 5.99LDD360 pKa = 5.61IIVGSDD366 pKa = 3.34VTWEE370 pKa = 4.17YY371 pKa = 11.23EE372 pKa = 3.91VTNIGNVPLSGISVNDD388 pKa = 3.08SDD390 pKa = 5.51EE391 pKa = 4.45NVTPAYY397 pKa = 10.76VSGDD401 pKa = 3.55TNEE404 pKa = 5.7DD405 pKa = 3.39DD406 pKa = 4.55MLDD409 pKa = 3.66LTEE412 pKa = 3.88TWIFKK417 pKa = 10.75ASGTAIQGSYY427 pKa = 11.08SNTGYY432 pKa = 11.03VSGTFEE438 pKa = 4.44EE439 pKa = 4.93STATADD445 pKa = 4.42DD446 pKa = 3.97DD447 pKa = 4.9SNYY450 pKa = 10.4FGYY453 pKa = 10.32IPDD456 pKa = 4.09APAIQITKK464 pKa = 6.43TTNGDD469 pKa = 3.39DD470 pKa = 3.65GLEE473 pKa = 3.94IEE475 pKa = 4.89VGSEE479 pKa = 3.75ITWSYY484 pKa = 11.21EE485 pKa = 3.49VTNKK489 pKa = 10.47GNVALGNITVTDD501 pKa = 3.84SDD503 pKa = 3.76EE504 pKa = 5.35SITPVYY510 pKa = 10.87VSGDD514 pKa = 3.43ANTDD518 pKa = 3.14GNLDD522 pKa = 3.62LTEE525 pKa = 3.92TWIYY529 pKa = 10.2EE530 pKa = 4.16ATGTAIEE537 pKa = 4.49GNYY540 pKa = 10.44SNTGYY545 pKa = 11.05VSGTFEE551 pKa = 3.76EE552 pKa = 4.4ATYY555 pKa = 10.95RR556 pKa = 11.84DD557 pKa = 4.94DD558 pKa = 5.95DD559 pKa = 4.63DD560 pKa = 4.93SSYY563 pKa = 11.23FGYY566 pKa = 10.41IPDD569 pKa = 4.84DD570 pKa = 3.61PAIQIIKK577 pKa = 6.19TTNGNDD583 pKa = 3.45GLEE586 pKa = 4.05IEE588 pKa = 4.77VGSEE592 pKa = 3.44ITWRR596 pKa = 11.84YY597 pKa = 9.68EE598 pKa = 3.34ITNKK602 pKa = 10.42GNVALGNITVTDD614 pKa = 3.84SDD616 pKa = 3.76EE617 pKa = 5.35SITPVYY623 pKa = 10.87VSGDD627 pKa = 3.38ANTDD631 pKa = 2.9GKK633 pKa = 11.33LDD635 pKa = 4.12LKK637 pKa = 8.88EE638 pKa = 3.88TWIYY642 pKa = 10.39EE643 pKa = 4.17ATGTAIEE650 pKa = 4.56GNYY653 pKa = 10.55SNIGYY658 pKa = 10.07VSGSFGEE665 pKa = 4.6GTVNDD670 pKa = 4.49NDD672 pKa = 3.4DD673 pKa = 3.72SYY675 pKa = 10.37YY676 pKa = 10.36TGYY679 pKa = 10.55VPQEE683 pKa = 4.02PKK685 pKa = 10.26TPEE688 pKa = 3.14ISINKK693 pKa = 5.92TTNGSDD699 pKa = 3.3GSRR702 pKa = 11.84IRR704 pKa = 11.84VGTAITWEE712 pKa = 4.34YY713 pKa = 10.98KK714 pKa = 10.25VEE716 pKa = 4.19TVNGFDD722 pKa = 3.98IYY724 pKa = 11.4DD725 pKa = 3.61VDD727 pKa = 4.16VTDD730 pKa = 5.33SDD732 pKa = 4.05TDD734 pKa = 3.96LDD736 pKa = 3.9PQYY739 pKa = 11.79VSGDD743 pKa = 3.65DD744 pKa = 3.65GDD746 pKa = 5.1GVLEE750 pKa = 4.88PGEE753 pKa = 4.09TWIYY757 pKa = 9.95QATGTAVSGLYY768 pKa = 10.45EE769 pKa = 4.38NIGYY773 pKa = 7.51VTGYY777 pKa = 10.72AEE779 pKa = 4.26RR780 pKa = 11.84EE781 pKa = 4.3VPVSDD786 pKa = 4.81EE787 pKa = 4.27DD788 pKa = 3.86TSSYY792 pKa = 10.19TGYY795 pKa = 8.75TVTTTTTTRR804 pKa = 11.84RR805 pKa = 11.84TPDD808 pKa = 3.95PDD810 pKa = 4.09PGNIEE815 pKa = 3.56IFKK818 pKa = 10.9FLDD821 pKa = 3.28SDD823 pKa = 4.21GNGSFDD829 pKa = 4.31EE830 pKa = 4.35EE831 pKa = 4.55NEE833 pKa = 4.18FPFEE837 pKa = 4.3NIRR840 pKa = 11.84FQLYY844 pKa = 10.8DD845 pKa = 3.64EE846 pKa = 5.02DD847 pKa = 4.34KK848 pKa = 11.46GLIEE852 pKa = 4.15TASTDD857 pKa = 3.71DD858 pKa = 3.48EE859 pKa = 4.83GMLTFRR865 pKa = 11.84NLDD868 pKa = 3.15AGTYY872 pKa = 8.58YY873 pKa = 10.14IKK875 pKa = 10.4EE876 pKa = 3.88ARR878 pKa = 11.84NDD880 pKa = 3.5YY881 pKa = 10.94SITTGGFDD889 pKa = 3.26EE890 pKa = 4.62NGFYY894 pKa = 10.62EE895 pKa = 4.25VDD897 pKa = 3.23VDD899 pKa = 3.64EE900 pKa = 6.0DD901 pKa = 3.76EE902 pKa = 4.42TVEE905 pKa = 4.28IEE907 pKa = 3.93VGNYY911 pKa = 9.72RR912 pKa = 11.84EE913 pKa = 4.34VIPPQEE919 pKa = 4.67PPLGPPPEE927 pKa = 4.04VEE929 pKa = 4.44EE930 pKa = 3.99ILEE933 pKa = 4.27EE934 pKa = 4.27KK935 pKa = 10.43VPQGPPLPQTGEE947 pKa = 3.8IPPYY951 pKa = 8.51FAYY954 pKa = 10.22GIGSLLILAGAFMRR968 pKa = 11.84RR969 pKa = 11.84KK970 pKa = 9.42FF971 pKa = 3.46

Molecular weight: 106.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5S6R0|A0A1G5S6R0_9FIRM Uncharacterized protein OS=Acidaminobacter hydrogenoformans DSM 2784 OX=1120920 GN=SAMN03080599_03247 PE=4 SV=1
AA1 pKa = 7.98CITAFRR7 pKa = 11.84NWSKK11 pKa = 11.06EE12 pKa = 3.21ILNAFKK18 pKa = 10.81YY19 pKa = 10.34GYY21 pKa = 9.31TNGCTEE27 pKa = 4.25GFNNKK32 pKa = 8.96IKK34 pKa = 9.61VLKK37 pKa = 9.9RR38 pKa = 11.84ISYY41 pKa = 8.63GVRR44 pKa = 11.84NFMRR48 pKa = 11.84FRR50 pKa = 11.84NRR52 pKa = 11.84ILHH55 pKa = 4.88MCRR58 pKa = 3.49

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3277

0

3277

1050301

40

2971

320.5

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.57 ± 0.048

0.945 ± 0.018

5.287 ± 0.037

7.245 ± 0.043

4.18 ± 0.029

7.59 ± 0.043

1.888 ± 0.019

7.172 ± 0.042

5.85 ± 0.036

10.127 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.051 ± 0.021

3.668 ± 0.029

3.831 ± 0.026

3.014 ± 0.022

4.719 ± 0.036

6.003 ± 0.031

5.297 ± 0.031

7.384 ± 0.034

0.844 ± 0.014

3.335 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski