Shigella phage SFN6B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Shigella virus SFN6B

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0XMY2|A0A2P0XMY2_9CAUD Uncharacterized protein OS=Shigella phage SFN6B OX=1785176 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.01KK3 pKa = 11.13VLDD6 pKa = 4.27AYY8 pKa = 10.06KK9 pKa = 10.78NLAITVGAAVYY20 pKa = 9.96DD21 pKa = 3.94AAACGIHH28 pKa = 7.56DD29 pKa = 3.94ISLDD33 pKa = 3.86NIYY36 pKa = 10.89DD37 pKa = 4.01ALDD40 pKa = 3.69EE41 pKa = 4.28LAALYY46 pKa = 10.96GMDD49 pKa = 4.95LEE51 pKa = 4.79LAATAFKK58 pKa = 10.44EE59 pKa = 4.24HH60 pKa = 6.94NDD62 pKa = 3.63LAAHH66 pKa = 6.58ADD68 pKa = 3.59KK69 pKa = 11.41LKK71 pKa = 11.01GDD73 pKa = 4.21DD74 pKa = 3.6LAVIRR79 pKa = 11.84VVGTLSVGLAEE90 pKa = 4.96IGSCIYY96 pKa = 10.87DD97 pKa = 3.05VDD99 pKa = 3.62QSLRR103 pKa = 11.84TPEE106 pKa = 4.5VIGDD110 pKa = 3.64MLGTVLVLSEE120 pKa = 5.2LEE122 pKa = 4.28GSDD125 pKa = 3.41AA126 pKa = 5.04

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0XMX1|A0A2P0XMX1_9CAUD Putative DNA helicase OS=Shigella phage SFN6B OX=1785176 PE=4 SV=1
MM1 pKa = 7.34ASKK4 pKa = 10.57YY5 pKa = 10.45GKK7 pKa = 9.95NPEE10 pKa = 4.08TLLMRR15 pKa = 11.84RR16 pKa = 11.84QQPTIEE22 pKa = 4.18GLARR26 pKa = 11.84EE27 pKa = 4.45YY28 pKa = 10.62SAKK31 pKa = 10.43AALRR35 pKa = 11.84QHH37 pKa = 6.15YY38 pKa = 7.59EE39 pKa = 3.85KK40 pKa = 10.33QAKK43 pKa = 8.74RR44 pKa = 11.84LGMTLRR50 pKa = 11.84GYY52 pKa = 8.15CQRR55 pKa = 11.84FNVRR59 pKa = 11.84GVVV62 pKa = 3.11

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13486

38

1420

275.2

30.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.455 ± 0.558

1.238 ± 0.232

6.295 ± 0.283

5.784 ± 0.331

2.966 ± 0.164

7.504 ± 0.266

1.906 ± 0.153

4.62 ± 0.259

4.931 ± 0.295

8.668 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.161

4.219 ± 0.233

3.752 ± 0.251

4.627 ± 0.367

5.828 ± 0.338

6.392 ± 0.255

5.739 ± 0.242

6.777 ± 0.185

1.528 ± 0.13

3.997 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski