Formosa sediminum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Formosa

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516GTL2|A0A516GTL2_9FLAO DUF4907 domain-containing protein OS=Formosa sediminum OX=2594004 GN=FNB79_13015 PE=4 SV=1
MM1 pKa = 7.08ITNSTKK7 pKa = 10.73VLVLCALALSLFNCSSDD24 pKa = 4.88DD25 pKa = 4.75DD26 pKa = 4.65DD27 pKa = 6.43DD28 pKa = 4.71RR29 pKa = 11.84GNWVEE34 pKa = 4.26RR35 pKa = 11.84SVFDD39 pKa = 3.42GVPRR43 pKa = 11.84SNVVGFVIDD52 pKa = 3.47EE53 pKa = 4.31LGYY56 pKa = 10.29MGTGYY61 pKa = 10.86DD62 pKa = 3.69GDD64 pKa = 4.73DD65 pKa = 3.62YY66 pKa = 11.78LVDD69 pKa = 4.15FWQYY73 pKa = 11.52NIAGDD78 pKa = 3.83YY79 pKa = 8.41WVQKK83 pKa = 10.98ADD85 pKa = 3.7FPGTEE90 pKa = 3.83RR91 pKa = 11.84SAATGFALDD100 pKa = 3.45GLGYY104 pKa = 10.69LGTGYY109 pKa = 10.83DD110 pKa = 4.08GVDD113 pKa = 3.42EE114 pKa = 6.33LSDD117 pKa = 3.51FWQYY121 pKa = 11.61NPSTNTWTQKK131 pKa = 10.36ADD133 pKa = 3.32FMGGVRR139 pKa = 11.84QAAIGFGANGTGYY152 pKa = 10.88VGTGYY157 pKa = 10.89DD158 pKa = 3.69GDD160 pKa = 3.92NDD162 pKa = 3.77RR163 pKa = 11.84KK164 pKa = 10.76DD165 pKa = 3.62FYY167 pKa = 11.03KK168 pKa = 11.01YY169 pKa = 11.09NPTTDD174 pKa = 2.84TWSEE178 pKa = 3.74LVGFGGEE185 pKa = 3.76KK186 pKa = 10.02RR187 pKa = 11.84RR188 pKa = 11.84FGTTFAIGDD197 pKa = 3.72KK198 pKa = 10.59VYY200 pKa = 10.69IGTGVSNGLYY210 pKa = 8.68KK211 pKa = 10.35TDD213 pKa = 3.59FWEE216 pKa = 4.35FDD218 pKa = 3.3PATEE222 pKa = 4.25VFTSKK227 pKa = 11.01LDD229 pKa = 3.69LDD231 pKa = 4.33EE232 pKa = 5.29EE233 pKa = 4.89DD234 pKa = 5.06DD235 pKa = 4.18YY236 pKa = 11.99SITRR240 pKa = 11.84SNAVGFSIDD249 pKa = 3.14GLGYY253 pKa = 10.01IVSGYY258 pKa = 10.61NGGVLNSTWEE268 pKa = 3.94YY269 pKa = 11.59DD270 pKa = 3.35PGKK273 pKa = 10.4DD274 pKa = 3.04EE275 pKa = 4.53WEE277 pKa = 5.36DD278 pKa = 3.21ITGLEE283 pKa = 4.74GYY285 pKa = 9.83SRR287 pKa = 11.84QDD289 pKa = 2.94ALAFSTGTRR298 pKa = 11.84AFVLLGRR305 pKa = 11.84SGSLYY310 pKa = 10.73LDD312 pKa = 4.16DD313 pKa = 5.42NYY315 pKa = 11.08EE316 pKa = 4.23LFPQEE321 pKa = 5.74DD322 pKa = 4.3YY323 pKa = 11.62DD324 pKa = 6.31DD325 pKa = 4.29EE326 pKa = 4.69DD327 pKa = 3.54

Molecular weight: 36.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516GRJ6|A0A516GRJ6_9FLAO Inosine-5'-monophosphate dehydrogenase OS=Formosa sediminum OX=2594004 GN=guaB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.6LSVSSEE48 pKa = 4.04SRR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.69KK53 pKa = 9.55

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3315

0

3315

1148853

14

2542

346.6

39.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.414 ± 0.044

0.751 ± 0.013

5.673 ± 0.033

6.323 ± 0.038

5.119 ± 0.034

6.148 ± 0.042

1.929 ± 0.02

8.111 ± 0.04

7.599 ± 0.051

9.477 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.019

6.244 ± 0.044

3.332 ± 0.023

3.383 ± 0.023

3.164 ± 0.021

6.497 ± 0.036

6.271 ± 0.041

6.197 ± 0.03

1.017 ± 0.013

4.26 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski