Rhodanobacter fulvus Jip2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Rhodanobacter; Rhodanobacter fulvus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4VJT7|I4VJT7_9GAMM Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Rhodanobacter fulvus Jip2 OX=1163408 GN=fabV PE=3 SV=1
MM1 pKa = 5.71QTKK4 pKa = 8.62TRR6 pKa = 11.84FTRR9 pKa = 11.84STVAAALIAAFAAVSGTAIAGTDD32 pKa = 3.42TDD34 pKa = 4.16NLSVSATVTASCTVDD49 pKa = 2.96STTEE53 pKa = 4.1VAFGTYY59 pKa = 10.57DD60 pKa = 3.51PVADD64 pKa = 4.05NASTGIDD71 pKa = 3.39LTTSSGAIATTCTNGSSATITLGQGANSDD100 pKa = 3.76TGSTDD105 pKa = 3.26TAPLRR110 pKa = 11.84RR111 pKa = 11.84MVGATNTDD119 pKa = 3.3EE120 pKa = 4.12YY121 pKa = 11.26LSYY124 pKa = 11.03SLCQDD129 pKa = 2.99STACATVWGNDD140 pKa = 2.95AGTGQVVTGTGASDD154 pKa = 3.55SVVVYY159 pKa = 9.55GSAPKK164 pKa = 9.78GQNVKK169 pKa = 10.7ADD171 pKa = 3.87TYY173 pKa = 11.06SDD175 pKa = 3.66TVLVTVTFF183 pKa = 4.28

Molecular weight: 18.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4VLB8|I4VLB8_9GAMM Uncharacterized protein OS=Rhodanobacter fulvus Jip2 OX=1163408 GN=UU9_14420 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.06LKK11 pKa = 10.38RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATADD26 pKa = 3.12GRR28 pKa = 11.84KK29 pKa = 9.4IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LIPP44 pKa = 4.02

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3400

0

3400

1141324

33

2876

335.7

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.646 ± 0.057

0.829 ± 0.014

5.904 ± 0.034

4.91 ± 0.037

3.408 ± 0.026

8.428 ± 0.047

2.519 ± 0.021

4.324 ± 0.029

2.924 ± 0.035

10.91 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.02

2.662 ± 0.032

5.236 ± 0.032

3.897 ± 0.027

7.119 ± 0.046

5.398 ± 0.032

5.178 ± 0.042

7.436 ± 0.035

1.532 ± 0.019

2.428 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski