Azotobacter chroococcum NCIMB 8003

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Azotobacter group; Azotobacter; Azotobacter chroococcum

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4WLT7|A0A0C4WLT7_9GAMM ABC transporter permease protein OS=Azotobacter chroococcum NCIMB 8003 OX=1328314 GN=Achr_17350 PE=3 SV=1
MM1 pKa = 7.13YY2 pKa = 10.51LITEE6 pKa = 4.25SGLNDD11 pKa = 3.59KK12 pKa = 10.99APYY15 pKa = 10.27DD16 pKa = 3.76PALLAFIHH24 pKa = 6.46EE25 pKa = 4.68GDD27 pKa = 4.42EE28 pKa = 3.77IRR30 pKa = 11.84NPYY33 pKa = 10.26LSPCGRR39 pKa = 11.84YY40 pKa = 9.05EE41 pKa = 3.98VDD43 pKa = 3.23PVAAYY48 pKa = 10.11GFEE51 pKa = 4.23EE52 pKa = 4.84VWTGGNCRR60 pKa = 11.84ALDD63 pKa = 4.62LILPDD68 pKa = 3.79GCVLRR73 pKa = 11.84LTNEE77 pKa = 4.57DD78 pKa = 4.07GLCIPDD84 pKa = 3.84PDD86 pKa = 3.67EE87 pKa = 4.25WEE89 pKa = 4.04SAIIGRR95 pKa = 11.84LSSDD99 pKa = 2.93HH100 pKa = 7.52DD101 pKa = 4.36EE102 pKa = 4.59IAWCVLEE109 pKa = 4.85EE110 pKa = 4.22VPSTIGRR117 pKa = 3.75

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4WUE4|A0A0C4WUE4_9GAMM Conjugal transfer protein TraL OS=Azotobacter chroococcum NCIMB 8003 OX=1328314 GN=traL PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4353

0

4353

1419050

25

3611

326.0

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.758 ± 0.049

1.071 ± 0.012

5.232 ± 0.031

6.354 ± 0.034

3.517 ± 0.023

8.27 ± 0.039

2.355 ± 0.017

4.296 ± 0.026

3.013 ± 0.036

12.298 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.015

2.524 ± 0.024

5.222 ± 0.029

4.174 ± 0.029

7.643 ± 0.038

5.272 ± 0.026

4.314 ± 0.023

6.737 ± 0.031

1.466 ± 0.018

2.425 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski