Candidatus Nanopelagicus abundans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Candidatus Nanopelagicales; Candidatus Nanopelagicaceae; Candidatus Nanopelagicus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249L5M6|A0A249L5M6_9ACTN Fumarate hydratase class I OS=Candidatus Nanopelagicus abundans OX=1884916 GN=B1sIIB91_04935 PE=3 SV=1
MM1 pKa = 7.58NFNQNDD7 pKa = 5.41LIPCEE12 pKa = 3.9QVLPNIVLYY21 pKa = 10.11IDD23 pKa = 4.85HH24 pKa = 7.52EE25 pKa = 4.36IFDD28 pKa = 4.36TQEE31 pKa = 3.48LSAVEE36 pKa = 3.99IHH38 pKa = 6.98FGQCPSCRR46 pKa = 11.84TYY48 pKa = 10.16MEE50 pKa = 5.17RR51 pKa = 11.84EE52 pKa = 4.18DD53 pKa = 3.82ATLTLMKK60 pKa = 10.71NLLCNSLIEE69 pKa = 4.27SAPEE73 pKa = 3.66EE74 pKa = 4.04LCNRR78 pKa = 11.84INQQTQDD85 pKa = 4.44LYY87 pKa = 10.84NQMMQEE93 pKa = 4.09GDD95 pKa = 3.76SQEE98 pKa = 4.17VTEE101 pKa = 4.17ITYY104 pKa = 8.06TQTTYY109 pKa = 11.14TEE111 pKa = 4.23ISAEE115 pKa = 3.95GTTQIEE121 pKa = 4.13ITSEE125 pKa = 3.29IRR127 pKa = 11.84RR128 pKa = 11.84QFPLEE133 pKa = 3.79

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249L630|A0A249L630_9ACTN Uncharacterized protein OS=Candidatus Nanopelagicus abundans OX=1884916 GN=B1sIIB91_05055 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.52RR3 pKa = 11.84TFQPNVRR10 pKa = 11.84KK11 pKa = 9.49RR12 pKa = 11.84AKK14 pKa = 9.83KK15 pKa = 8.78HH16 pKa = 4.25GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84GVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.3ISAA44 pKa = 3.71

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1145

0

1145

363772

44

1506

317.7

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.795 ± 0.072

0.738 ± 0.019

5.354 ± 0.054

5.762 ± 0.06

3.802 ± 0.052

7.752 ± 0.064

1.61 ± 0.03

7.245 ± 0.057

6.174 ± 0.061

10.029 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.031

3.888 ± 0.043

4.128 ± 0.044

3.112 ± 0.04

4.772 ± 0.051

7.022 ± 0.057

5.534 ± 0.049

7.566 ± 0.054

1.144 ± 0.026

2.464 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski