methanotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N1YB35|A0A6N1YB35_9BACT Methyltransf_25 domain-containing protein OS=methanotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev) OX=343235 GN=BPUTEOMOX_2018 PE=4 SV=1
MM1 pKa = 8.17ADD3 pKa = 4.3PIIFSDD9 pKa = 3.55SAAAKK14 pKa = 9.93VGALIAEE21 pKa = 4.58EE22 pKa = 4.96GNDD25 pKa = 3.67SLKK28 pKa = 10.81LRR30 pKa = 11.84VYY32 pKa = 10.52ISGGGCSGFQYY43 pKa = 10.93GFTFDD48 pKa = 4.82EE49 pKa = 4.76EE50 pKa = 4.53VADD53 pKa = 6.25DD54 pKa = 3.9DD55 pKa = 4.46TQVEE59 pKa = 4.4NGGVTVLVDD68 pKa = 3.62AMSIQYY74 pKa = 10.98LNGAEE79 pKa = 4.0IDD81 pKa = 3.99YY82 pKa = 11.28KK83 pKa = 11.08EE84 pKa = 4.83DD85 pKa = 3.16LSGAQFVIRR94 pKa = 11.84NPNASTTCGCGSSFSVV110 pKa = 3.54

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N1YDJ2|A0A6N1YDJ2_9BACT General secretion pathway protein I OS=methanotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev) OX=343235 GN=BPUTEOMOX_2121 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.24IKK11 pKa = 10.37RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84HH40 pKa = 4.25TLALL44 pKa = 3.89

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3063

0

3063

911241

37

1941

297.5

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.558 ± 0.045

1.022 ± 0.018

5.266 ± 0.029

6.113 ± 0.04

4.198 ± 0.032

6.407 ± 0.043

2.286 ± 0.021

7.204 ± 0.041

6.024 ± 0.038

10.7 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.019

4.399 ± 0.032

3.817 ± 0.026

4.839 ± 0.04

4.362 ± 0.033

6.334 ± 0.031

5.246 ± 0.033

6.428 ± 0.041

1.177 ± 0.017

3.126 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski