Dorea sp. 5-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; unclassified Dorea

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9N8C1|R9N8C1_9FIRM T2SSF domain-containing protein OS=Dorea sp. 5-2 OX=1235798 GN=C817_03003 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.08RR3 pKa = 11.84RR4 pKa = 11.84AIAALLTAAMSVSLFTGCHH23 pKa = 6.56DD24 pKa = 4.31SSQEE28 pKa = 3.74TAAVDD33 pKa = 4.46VEE35 pKa = 4.31NTDD38 pKa = 3.46YY39 pKa = 11.47DD40 pKa = 3.66PEE42 pKa = 4.11EE43 pKa = 3.98AVKK46 pKa = 10.74DD47 pKa = 4.12FEE49 pKa = 4.44FGEE52 pKa = 4.19LSEE55 pKa = 4.33EE56 pKa = 4.13EE57 pKa = 3.98KK58 pKa = 11.08NYY60 pKa = 9.03TIEE63 pKa = 4.02MGYY66 pKa = 9.93FNCDD70 pKa = 2.83HH71 pKa = 6.36MVGSIIGDD79 pKa = 3.25KK80 pKa = 10.95AGIYY84 pKa = 7.91EE85 pKa = 4.11ALGLKK90 pKa = 10.52VNVTKK95 pKa = 10.83SSEE98 pKa = 4.16TLKK101 pKa = 11.1ALTSGAMDD109 pKa = 3.35VGYY112 pKa = 8.94TGVTGAVRR120 pKa = 11.84AVNQGAPFFIAAANHH135 pKa = 6.1MGGSMYY141 pKa = 10.75LVASNEE147 pKa = 3.89IKK149 pKa = 9.72TAEE152 pKa = 4.07DD153 pKa = 3.51LKK155 pKa = 11.37GKK157 pKa = 7.27TLSITATPDD166 pKa = 2.92IDD168 pKa = 3.78PTILTWEE175 pKa = 4.16QEE177 pKa = 3.99IGMSSNPEE185 pKa = 3.83DD186 pKa = 4.07YY187 pKa = 10.74EE188 pKa = 4.17IVDD191 pKa = 4.01MGQQDD196 pKa = 3.71AMFALKK202 pKa = 10.57AEE204 pKa = 4.34QIDD207 pKa = 4.16AFSCCDD213 pKa = 3.64PYY215 pKa = 11.86ASIAEE220 pKa = 4.13FEE222 pKa = 4.56GFGHH226 pKa = 6.96IMAIDD231 pKa = 3.85WAAPDD236 pKa = 3.5ISSDD240 pKa = 4.42SEE242 pKa = 4.02YY243 pKa = 11.2DD244 pKa = 3.81DD245 pKa = 4.53WGLCCVYY252 pKa = 11.27AMNTDD257 pKa = 4.48FSKK260 pKa = 11.01NCPEE264 pKa = 3.98LARR267 pKa = 11.84RR268 pKa = 11.84LIFAHH273 pKa = 6.28SLAIKK278 pKa = 10.26YY279 pKa = 8.91MYY281 pKa = 8.51EE282 pKa = 3.98HH283 pKa = 7.8PYY285 pKa = 8.78NAAMMFADD293 pKa = 5.07GFDD296 pKa = 3.48VDD298 pKa = 4.54PYY300 pKa = 10.18VALRR304 pKa = 11.84TIYY307 pKa = 9.87MKK309 pKa = 10.15TVSEE313 pKa = 4.19GRR315 pKa = 11.84TLTWKK320 pKa = 10.75FSDD323 pKa = 3.86PNVDD327 pKa = 4.16SFLEE331 pKa = 4.55YY332 pKa = 9.14YY333 pKa = 8.36TQYY336 pKa = 11.01SQIPEE341 pKa = 4.11EE342 pKa = 4.9EE343 pKa = 3.89IPLINDD349 pKa = 3.32RR350 pKa = 11.84NTLISSEE357 pKa = 4.1VLEE360 pKa = 4.29AAEE363 pKa = 4.48IEE365 pKa = 4.64DD366 pKa = 3.74FQTYY370 pKa = 9.99IKK372 pKa = 10.86DD373 pKa = 3.67VVDD376 pKa = 4.89PIMPLGTTFEE386 pKa = 4.15DD387 pKa = 3.62WYY389 pKa = 10.02EE390 pKa = 3.73ISKK393 pKa = 10.69EE394 pKa = 3.47IDD396 pKa = 4.02GISDD400 pKa = 3.31EE401 pKa = 4.51DD402 pKa = 3.86AVDD405 pKa = 3.59ISDD408 pKa = 3.62TATPYY413 pKa = 11.09LNEE416 pKa = 4.23NLDD419 pKa = 3.71EE420 pKa = 4.35RR421 pKa = 11.84TSNN424 pKa = 3.44

Molecular weight: 47.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9MKG5|R9MKG5_9FIRM Uncharacterized protein OS=Dorea sp. 5-2 OX=1235798 GN=C817_05762 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.88VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5824

0

5824

1704686

21

3121

292.7

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.372 ± 0.033

1.569 ± 0.013

5.625 ± 0.027

8.033 ± 0.042

4.035 ± 0.021

7.072 ± 0.032

1.804 ± 0.014

7.199 ± 0.03

6.729 ± 0.028

8.958 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.164 ± 0.017

4.09 ± 0.021

3.25 ± 0.019

3.349 ± 0.018

5.105 ± 0.03

5.711 ± 0.026

5.051 ± 0.023

6.604 ± 0.023

0.977 ± 0.011

4.306 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski