Phytophthora megakarya

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A225WHI1|A0A225WHI1_9STRA Exocyst subunit Exo70 family protein OS=Phytophthora megakarya OX=4795 GN=PHMEG_0009139 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 5.42GSTTYY7 pKa = 10.37CYY9 pKa = 10.67YY10 pKa = 10.22ISEE13 pKa = 4.98KK14 pKa = 10.55YY15 pKa = 10.25SQEE18 pKa = 3.91QPVEE22 pKa = 4.17DD23 pKa = 4.79DD24 pKa = 3.97VLVMDD29 pKa = 5.24QGTKK33 pKa = 10.33CPITIRR39 pKa = 11.84VDD41 pKa = 3.02IADD44 pKa = 4.06EE45 pKa = 4.31LEE47 pKa = 4.24KK48 pKa = 11.09NALIPLRR55 pKa = 11.84YY56 pKa = 7.58TATLDD61 pKa = 3.63TSTADD66 pKa = 3.61ANFEE70 pKa = 4.09FPDD73 pKa = 5.0PIVKK77 pKa = 9.97VPVPDD82 pKa = 3.63YY83 pKa = 11.63LMSKK87 pKa = 8.83TNASTEE93 pKa = 4.28SSSSSDD99 pKa = 2.91TSFDD103 pKa = 3.39VAVANVVMCDD113 pKa = 3.01WRR115 pKa = 11.84SCNLFTTAGEE125 pKa = 4.18STTYY129 pKa = 10.47YY130 pKa = 10.61SSSNNPNDD138 pKa = 3.31FSDD141 pKa = 3.57NVAVFGSEE149 pKa = 3.76EE150 pKa = 4.0LVIPKK155 pKa = 9.91DD156 pKa = 3.66GKK158 pKa = 8.09YY159 pKa = 8.58TGYY162 pKa = 10.17VHH164 pKa = 7.06LVVNIGGNSRR174 pKa = 11.84ADD176 pKa = 3.23FVTFFPVQIGDD187 pKa = 3.76VAGTTPSSITTDD199 pKa = 3.1GTTTYY204 pKa = 10.06CWTSSDD210 pKa = 3.42VSVFDD215 pKa = 4.5PSVSGDD221 pKa = 3.22LTIRR225 pKa = 11.84TGEE228 pKa = 4.12YY229 pKa = 10.38CPGAMSMSLSSTDD242 pKa = 3.34VYY244 pKa = 11.46VGDD247 pKa = 5.03SIDD250 pKa = 3.76IAWMLDD256 pKa = 3.37MTDD259 pKa = 3.71SSTDD263 pKa = 3.32DD264 pKa = 3.24STLIAEE270 pKa = 4.67VSTTDD275 pKa = 4.45AIKK278 pKa = 10.58DD279 pKa = 3.7PRR281 pKa = 11.84NDD283 pKa = 2.98IYY285 pKa = 11.51SVVPVSVFSGCQRR298 pKa = 11.84NLAGANCSTYY308 pKa = 10.58TGDD311 pKa = 3.6EE312 pKa = 4.18SSTFAIAEE320 pKa = 4.01YY321 pKa = 10.87DD322 pKa = 3.74DD323 pKa = 4.28MNFTSDD329 pKa = 3.96AISYY333 pKa = 6.83STNYY337 pKa = 9.03TFEE340 pKa = 4.32NSGQYY345 pKa = 10.51TMFGRR350 pKa = 11.84VAMVTVDD357 pKa = 3.5GEE359 pKa = 4.48RR360 pKa = 11.84IDD362 pKa = 3.55MAIYY366 pKa = 10.34SSVTVSVDD374 pKa = 3.08GGSSGSNLFLYY385 pKa = 10.24IGIGIGAAILLGGLLFCYY403 pKa = 8.84MKK405 pKa = 10.51KK406 pKa = 10.15RR407 pKa = 11.84RR408 pKa = 11.84NNVNTKK414 pKa = 10.02DD415 pKa = 3.32IPFRR419 pKa = 11.84QPVGVMDD426 pKa = 4.65MDD428 pKa = 4.26RR429 pKa = 11.84SSDD432 pKa = 3.44FTMASSQFLTHH443 pKa = 6.24KK444 pKa = 9.71TPAQLHH450 pKa = 5.7GLDD453 pKa = 3.39MSGDD457 pKa = 3.58YY458 pKa = 10.47SANNTAPSYY467 pKa = 10.53LAINAGDD474 pKa = 3.45TSIFDD479 pKa = 3.74KK480 pKa = 10.87TISAEE485 pKa = 3.98PGQMAEE491 pKa = 4.29SVEE494 pKa = 4.29SSEE497 pKa = 4.89SFSLNPFEE505 pKa = 4.5RR506 pKa = 11.84ASFAGLSDD514 pKa = 4.24DD515 pKa = 4.13VASSRR520 pKa = 11.84PSDD523 pKa = 3.23MTKK526 pKa = 10.42FSFQSGYY533 pKa = 11.02DD534 pKa = 3.98DD535 pKa = 4.17GSEE538 pKa = 4.22WEE540 pKa = 5.08FDD542 pKa = 3.56TQNIGGDD549 pKa = 3.41RR550 pKa = 11.84GSDD553 pKa = 3.36FSDD556 pKa = 4.04FASNPGTSLPTTGTYY571 pKa = 10.72EE572 pKa = 4.14MGRR575 pKa = 11.84GMPILEE581 pKa = 4.4EE582 pKa = 4.6DD583 pKa = 4.75DD584 pKa = 5.27RR585 pKa = 11.84DD586 pKa = 3.61TDD588 pKa = 3.73TYY590 pKa = 11.41NPQHH594 pKa = 6.5QSTTTVDD601 pKa = 3.44PRR603 pKa = 11.84STTSSGWTVQQ613 pKa = 3.03

Molecular weight: 66.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A225UGQ9|A0A225UGQ9_9STRA Reverse transcriptase OS=Phytophthora megakarya OX=4795 GN=PHMEG_00038855 PE=4 SV=1
MM1 pKa = 7.61LISRR5 pKa = 11.84LHH7 pKa = 5.39GAPRR11 pKa = 11.84LLLLLRR17 pKa = 11.84SLKK20 pKa = 10.17VRR22 pKa = 11.84LPRR25 pKa = 11.84LPKK28 pKa = 9.83RR29 pKa = 11.84ARR31 pKa = 11.84STLSALLQLLLRR43 pKa = 11.84QQ44 pKa = 3.83

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34747

0

34747

10966191

9

7597

315.6

35.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.082 ± 0.013

1.667 ± 0.007

5.894 ± 0.011

6.482 ± 0.013

3.719 ± 0.008

5.844 ± 0.014

2.469 ± 0.006

4.466 ± 0.01

5.359 ± 0.015

9.196 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.007

3.753 ± 0.008

4.734 ± 0.011

4.078 ± 0.01

6.606 ± 0.013

7.73 ± 0.017

6.044 ± 0.012

7.063 ± 0.012

1.427 ± 0.005

2.788 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski