Beihai weivirus-like virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLD3|A0A1L3KLD3_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 20 OX=1922749 PE=4 SV=1
MM1 pKa = 7.62AEE3 pKa = 3.41AAEE6 pKa = 4.19RR7 pKa = 11.84VNAQQAAFRR16 pKa = 11.84DD17 pKa = 4.23GTTITSTVVEE27 pKa = 4.43APGAASTSGQAEE39 pKa = 4.13GAEE42 pKa = 4.26VGTRR46 pKa = 11.84VARR49 pKa = 11.84PRR51 pKa = 11.84FPSMSEE57 pKa = 3.75EE58 pKa = 4.04EE59 pKa = 4.42VFLFSNDD66 pKa = 3.08PRR68 pKa = 11.84NLEE71 pKa = 3.93AANTLRR77 pKa = 11.84NKK79 pKa = 10.3GVGVHH84 pKa = 5.48QPSEE88 pKa = 4.07KK89 pKa = 9.78EE90 pKa = 3.55AAVRR94 pKa = 11.84DD95 pKa = 3.85EE96 pKa = 5.37LVDD99 pKa = 3.67VLCDD103 pKa = 3.26MVFTKK108 pKa = 10.59RR109 pKa = 11.84EE110 pKa = 4.02SEE112 pKa = 4.16KK113 pKa = 11.39AMMEE117 pKa = 4.17YY118 pKa = 10.75EE119 pKa = 4.5SLTKK123 pKa = 9.8TALPRR128 pKa = 11.84KK129 pKa = 9.72LSEE132 pKa = 4.06EE133 pKa = 4.39QKK135 pKa = 10.93LQWQQDD141 pKa = 3.3ALAAAEE147 pKa = 4.44GDD149 pKa = 3.53GLSYY153 pKa = 11.34SKK155 pKa = 10.91FIDD158 pKa = 3.5AFVKK162 pKa = 10.89AEE164 pKa = 3.97VSAKK168 pKa = 10.0PKK170 pKa = 9.53PRR172 pKa = 11.84PIANHH177 pKa = 5.93KK178 pKa = 9.6EE179 pKa = 3.51IRR181 pKa = 11.84LCALAKK187 pKa = 10.08CAWTYY192 pKa = 8.9EE193 pKa = 4.17HH194 pKa = 7.06VLFRR198 pKa = 11.84RR199 pKa = 11.84MNKK202 pKa = 9.1MSIKK206 pKa = 10.31HH207 pKa = 4.63RR208 pKa = 11.84TKK210 pKa = 10.7SEE212 pKa = 3.91ALSDD216 pKa = 3.71VANALSSMKK225 pKa = 10.39NGRR228 pKa = 11.84WCEE231 pKa = 3.69NDD233 pKa = 2.77LTAFEE238 pKa = 4.84FGISAKK244 pKa = 10.58LKK246 pKa = 9.75ACEE249 pKa = 3.9CAILRR254 pKa = 11.84HH255 pKa = 5.45VASQIGIEE263 pKa = 4.41DD264 pKa = 3.59VGSLLFEE271 pKa = 3.78RR272 pKa = 11.84VVNDD276 pKa = 3.47RR277 pKa = 11.84TKK279 pKa = 8.78TCVWSMRR286 pKa = 11.84YY287 pKa = 7.83TDD289 pKa = 3.38EE290 pKa = 4.31TGEE293 pKa = 3.99KK294 pKa = 9.02RR295 pKa = 11.84TFRR298 pKa = 11.84LILPTVMRR306 pKa = 11.84EE307 pKa = 3.8SGDD310 pKa = 3.56RR311 pKa = 11.84LTSSGNFLQNLIAWCSFLLAPGTVRR336 pKa = 11.84KK337 pKa = 9.9SVEE340 pKa = 3.74SLLRR344 pKa = 11.84TRR346 pKa = 11.84GEE348 pKa = 3.9HH349 pKa = 5.77MFYY352 pKa = 10.88CSARR356 pKa = 11.84DD357 pKa = 3.6GKK359 pKa = 10.95KK360 pKa = 10.31YY361 pKa = 9.81LAKK364 pKa = 10.51LVFEE368 pKa = 5.28GDD370 pKa = 3.58DD371 pKa = 3.31TLGRR375 pKa = 11.84LDD377 pKa = 3.75EE378 pKa = 4.74PVWEE382 pKa = 4.82PKK384 pKa = 10.16RR385 pKa = 11.84EE386 pKa = 3.97GATEE390 pKa = 4.01SLVDD394 pKa = 3.62DD395 pKa = 4.96FFYY398 pKa = 10.79RR399 pKa = 11.84WGWKK403 pKa = 9.42PKK405 pKa = 10.27LSWKK409 pKa = 8.81ATSGYY414 pKa = 10.86DD415 pKa = 3.29YY416 pKa = 11.33ARR418 pKa = 11.84VVGYY422 pKa = 10.35DD423 pKa = 3.2ILIKK427 pKa = 10.89DD428 pKa = 4.11GVAVRR433 pKa = 11.84DD434 pKa = 3.54GDD436 pKa = 4.03KK437 pKa = 10.97LVACPEE443 pKa = 3.76MRR445 pKa = 11.84RR446 pKa = 11.84LLNTKK451 pKa = 8.13QWTTTNVTPEE461 pKa = 3.93EE462 pKa = 4.3LKK464 pKa = 8.58TCNRR468 pKa = 11.84IFAATLAADD477 pKa = 4.67FTRR480 pKa = 11.84VEE482 pKa = 4.01PMYY485 pKa = 11.06SFLRR489 pKa = 11.84SMYY492 pKa = 10.49DD493 pKa = 3.23SNPGGKK499 pKa = 7.54TVTDD503 pKa = 3.56EE504 pKa = 4.02KK505 pKa = 11.0VRR507 pKa = 11.84EE508 pKa = 4.33HH509 pKa = 6.5FLMMTGEE516 pKa = 4.28LPAHH520 pKa = 6.56GSSLLSDD527 pKa = 3.36IAFPEE532 pKa = 3.92FDD534 pKa = 3.25GAGNDD539 pKa = 4.03VEE541 pKa = 4.58WKK543 pKa = 9.67ALARR547 pKa = 11.84VSCGDD552 pKa = 3.49FTDD555 pKa = 5.5LEE557 pKa = 4.46WATACAQPLHH567 pKa = 6.83DD568 pKa = 4.48RR569 pKa = 11.84HH570 pKa = 6.16GADD573 pKa = 4.65LATGMPASWVGTVAA587 pKa = 4.68

Molecular weight: 65.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLD3|A0A1L3KLD3_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 20 OX=1922749 PE=4 SV=1
MM1 pKa = 7.55AGKK4 pKa = 8.56GTRR7 pKa = 11.84KK8 pKa = 9.98NGNNARR14 pKa = 11.84RR15 pKa = 11.84APRR18 pKa = 11.84RR19 pKa = 11.84QGGRR23 pKa = 11.84AANRR27 pKa = 11.84STATRR32 pKa = 11.84VLATGVGASVPKK44 pKa = 10.36PFGSRR49 pKa = 11.84PGQGLACWDD58 pKa = 3.8AKK60 pKa = 10.85HH61 pKa = 6.5PSHH64 pKa = 7.04LALPRR69 pKa = 11.84AVGPYY74 pKa = 5.72TTVRR78 pKa = 11.84ATRR81 pKa = 11.84RR82 pKa = 11.84VQLNTHH88 pKa = 6.44CNVIGTFQEE97 pKa = 4.01GHH99 pKa = 6.54IAPSQAGDD107 pKa = 3.03WSEE110 pKa = 4.14VVLVADD116 pKa = 4.09VTATNPINGHH126 pKa = 6.07LNTRR130 pKa = 11.84SVTVDD135 pKa = 3.22LSGLGDD141 pKa = 3.88AATLVPSALSVQVMCPTALQTASGIIYY168 pKa = 9.82AGVMNTQAALGGRR181 pKa = 11.84VDD183 pKa = 5.98SWDD186 pKa = 3.26DD187 pKa = 3.03WCNKK191 pKa = 8.38FVQFQSPRR199 pKa = 11.84LLAASKK205 pKa = 10.23LALRR209 pKa = 11.84GIQINSYY216 pKa = 8.39PLNMTEE222 pKa = 4.06VSKK225 pKa = 8.46FTPLRR230 pKa = 11.84KK231 pKa = 9.37EE232 pKa = 3.51ADD234 pKa = 3.55LQFEE238 pKa = 4.53YY239 pKa = 10.66TDD241 pKa = 3.36QRR243 pKa = 11.84VEE245 pKa = 3.98PTGWAPIVIYY255 pKa = 10.64NPEE258 pKa = 4.03GTTLEE263 pKa = 4.02LLITVEE269 pKa = 3.69YY270 pKa = 10.11RR271 pKa = 11.84VRR273 pKa = 11.84FDD275 pKa = 4.28LDD277 pKa = 3.4HH278 pKa = 7.02PASASHH284 pKa = 5.36THH286 pKa = 6.25HH287 pKa = 6.96PVASDD292 pKa = 3.69STWDD296 pKa = 3.3RR297 pKa = 11.84MVRR300 pKa = 11.84SATALGNGVMDD311 pKa = 4.02IADD314 pKa = 3.62VVANTGMAVGRR325 pKa = 11.84AAMVGRR331 pKa = 11.84ALTNGAARR339 pKa = 11.84ALPALTMM346 pKa = 4.44

Molecular weight: 36.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

933

346

587

466.5

51.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.468 ± 0.869

1.822 ± 0.376

5.466 ± 0.476

6.109 ± 1.983

3.43 ± 0.795

7.181 ± 0.842

2.036 ± 0.319

2.787 ± 0.222

4.716 ± 1.359

8.467 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.105

3.751 ± 0.657

4.502 ± 0.723

2.68 ± 0.609

7.181 ± 0.188

6.538 ± 0.265

7.503 ± 0.66

7.61 ± 0.763

2.036 ± 0.171

1.929 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski