Xanthomonas phage XcP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Carpasinavirus; Xanthomonas virus XcP1

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7L8S9|A0A3S7L8S9_9CAUD Minor head protein OS=Xanthomonas phage XcP1 OX=2785027 GN=XcP1_006 PE=4 SV=1
MM1 pKa = 7.6IVEE4 pKa = 4.31FAFDD8 pKa = 3.52NGHH11 pKa = 5.59TEE13 pKa = 3.85QYY15 pKa = 8.63TVKK18 pKa = 9.12TLEE21 pKa = 4.06EE22 pKa = 4.65AYY24 pKa = 9.9SHH26 pKa = 7.51CIATWNKK33 pKa = 9.84SCSSDD38 pKa = 3.3DD39 pKa = 3.42MAEE42 pKa = 3.91FRR44 pKa = 11.84VGCAEE49 pKa = 4.17DD50 pKa = 4.01LNNAGVCHH58 pKa = 7.2RR59 pKa = 11.84IILDD63 pKa = 3.37

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7L8M1|A0A3S7L8M1_9CAUD DNA helicase OS=Xanthomonas phage XcP1 OX=2785027 GN=XcP1_052 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.99KK5 pKa = 10.35RR6 pKa = 11.84IRR8 pKa = 11.84SSAHH12 pKa = 5.43LVCLVIVIVLAVSAGIMYY30 pKa = 10.21GSSFAPRR37 pKa = 11.84QPGASGSWW45 pKa = 3.08

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

18642

45

942

230.1

25.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.31 ± 0.328

0.987 ± 0.116

5.917 ± 0.244

6.346 ± 0.334

3.911 ± 0.171

7.655 ± 0.349

1.641 ± 0.155

6.019 ± 0.219

5.933 ± 0.327

7.311 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.123

4.377 ± 0.232

4.259 ± 0.175

3.921 ± 0.163

5.348 ± 0.233

7.172 ± 0.33

5.402 ± 0.233

7.022 ± 0.209

1.245 ± 0.091

2.564 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski