Klebsiella phage CX1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R498|A0A6G8R498_9CAUD Uncharacterized protein OS=Klebsiella phage CX1 OX=2713210 GN=CX1_25 PE=4 SV=1
MM1 pKa = 7.48NIKK4 pKa = 9.93PGSIVEE10 pKa = 4.02LLEE13 pKa = 5.28LGPEE17 pKa = 4.44PIGPEE22 pKa = 3.66LSSYY26 pKa = 9.24FVPGSQHH33 pKa = 6.5RR34 pKa = 11.84VISYY38 pKa = 10.58CEE40 pKa = 3.92EE41 pKa = 3.7TWEE44 pKa = 4.73VEE46 pKa = 5.53LIDD49 pKa = 3.81PTNGSEE55 pKa = 4.2EE56 pKa = 4.37PGDD59 pKa = 4.17GITFFPGEE67 pKa = 3.93YY68 pKa = 10.18KK69 pKa = 10.88LIVEE73 pKa = 4.84

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R4X7|A0A6G8R4X7_9CAUD DNA-directed RNA polymerase OS=Klebsiella phage CX1 OX=2713210 GN=CX1_37 PE=3 SV=1
MM1 pKa = 8.21RR2 pKa = 11.84IITWAKK8 pKa = 7.54EE9 pKa = 3.66QYY11 pKa = 11.04AVFLLLRR18 pKa = 11.84AQRR21 pKa = 11.84LQKK24 pKa = 10.4RR25 pKa = 11.84ANDD28 pKa = 3.44WHH30 pKa = 6.22WAANSHH36 pKa = 5.18AHH38 pKa = 6.09RR39 pKa = 11.84VSLLGAEE46 pKa = 4.67ISAHH50 pKa = 6.46RR51 pKa = 11.84YY52 pKa = 8.83HH53 pKa = 7.31LTRR56 pKa = 11.84QCAKK60 pKa = 9.58SRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84AYY66 pKa = 10.62ALGAEE71 pKa = 4.45ATATEE76 pKa = 4.72TKK78 pKa = 10.56AHH80 pKa = 5.65TFISKK85 pKa = 10.33HH86 pKa = 5.06KK87 pKa = 10.7LKK89 pKa = 11.07GFDD92 pKa = 3.17

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13911

38

1232

235.8

26.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.632 ± 0.535

1.136 ± 0.207

6.132 ± 0.223

5.895 ± 0.308

3.055 ± 0.133

7.534 ± 0.258

1.783 ± 0.195

4.328 ± 0.161

4.809 ± 0.287

8.741 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.209

4.047 ± 0.189

3.731 ± 0.272

4.687 ± 0.398

5.945 ± 0.266

6.34 ± 0.319

5.981 ± 0.332

7.088 ± 0.185

1.459 ± 0.117

3.752 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski