Roseburia inulinivorans CAG:15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5HHW5|R5HHW5_9FIRM Signal transduction histidine kinase LytS OS=Roseburia inulinivorans CAG:15 OX=1263105 GN=BN501_01321 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.14KK3 pKa = 10.19KK4 pKa = 10.13VVSVILAMTMVASMAMGCGSGSDD27 pKa = 3.45TTTSDD32 pKa = 2.85NSTTTTTNDD41 pKa = 3.01AAASTEE47 pKa = 3.97ASDD50 pKa = 5.21AADD53 pKa = 3.61ATADD57 pKa = 3.63AANGDD62 pKa = 4.02LADD65 pKa = 4.42KK66 pKa = 10.79KK67 pKa = 11.16VGVCIYY73 pKa = 10.52QFADD77 pKa = 2.77NFMTLFRR84 pKa = 11.84GEE86 pKa = 4.23LEE88 pKa = 4.23NYY90 pKa = 9.72LVEE93 pKa = 4.63QGFSKK98 pKa = 11.16DD99 pKa = 3.2NITIVDD105 pKa = 4.16GANDD109 pKa = 3.29QATQTNQIQNFITQGVDD126 pKa = 3.06VLIINPVNSSSAEE139 pKa = 4.16TITDD143 pKa = 3.53MVVEE147 pKa = 4.27AGIPLVYY154 pKa = 10.23INRR157 pKa = 11.84EE158 pKa = 3.56PDD160 pKa = 2.96ASEE163 pKa = 3.85EE164 pKa = 4.49QRR166 pKa = 11.84WADD169 pKa = 3.68NNWDD173 pKa = 3.61VTYY176 pKa = 10.37VGCDD180 pKa = 2.96ARR182 pKa = 11.84QSGTYY187 pKa = 8.18QGEE190 pKa = 4.29MIADD194 pKa = 4.54LGLDD198 pKa = 3.47TVDD201 pKa = 3.35MNGNGKK207 pKa = 8.9IDD209 pKa = 3.93YY210 pKa = 10.73IMIEE214 pKa = 4.19GDD216 pKa = 3.83PEE218 pKa = 4.26NVDD221 pKa = 2.89AQYY224 pKa = 9.38RR225 pKa = 11.84TEE227 pKa = 3.99YY228 pKa = 10.35SIKK231 pKa = 10.34ALEE234 pKa = 4.37DD235 pKa = 3.12AGLEE239 pKa = 4.37VNCLDD244 pKa = 4.58DD245 pKa = 5.14QVGMWDD251 pKa = 3.26QATAQQLVANSLSQNGNDD269 pKa = 3.57IEE271 pKa = 5.43VVFCNNDD278 pKa = 2.86AMALGALQAIEE289 pKa = 4.22SAGRR293 pKa = 11.84TVGEE297 pKa = 4.54DD298 pKa = 2.93IYY300 pKa = 11.44LVGVDD305 pKa = 4.29ALSEE309 pKa = 4.0ALEE312 pKa = 4.43DD313 pKa = 3.83VLAGTMTGTVFNDD326 pKa = 3.53HH327 pKa = 7.14FSQSHH332 pKa = 5.07SAADD336 pKa = 3.36AAINYY341 pKa = 9.67LSGAGNEE348 pKa = 4.46HH349 pKa = 7.15YY350 pKa = 10.38IGCDD354 pKa = 3.34YY355 pKa = 11.75VKK357 pKa = 9.82VTKK360 pKa = 10.81DD361 pKa = 3.05NAQEE365 pKa = 3.74ILDD368 pKa = 3.88MVKK371 pKa = 10.74

Molecular weight: 39.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5I3G0|R5I3G0_9FIRM Uncharacterized protein OS=Roseburia inulinivorans CAG:15 OX=1263105 GN=BN501_00873 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2653

0

2653

844942

29

1532

318.5

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.461 ± 0.049

1.443 ± 0.018

5.761 ± 0.04

7.864 ± 0.055

4.206 ± 0.035

6.728 ± 0.04

1.769 ± 0.025

7.571 ± 0.043

6.996 ± 0.044

8.731 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.265 ± 0.023

4.647 ± 0.036

3.019 ± 0.023

3.315 ± 0.029

3.927 ± 0.036

5.594 ± 0.037

5.524 ± 0.042

6.985 ± 0.039

0.86 ± 0.014

4.33 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski