Mycobacterium sp. 1274756.6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A3JNC1|A0A1A3JNC1_9MYCO Rieske domain-containing protein OS=Mycobacterium sp. 1274756.6 OX=1834076 GN=A5643_02830 PE=4 SV=1
MM1 pKa = 7.17NPVHH5 pKa = 6.67RR6 pKa = 11.84RR7 pKa = 11.84AGAALTALTITAAGLLGLPPVSPLLDD33 pKa = 3.3LTTFAVHH40 pKa = 5.89LTADD44 pKa = 3.37ADD46 pKa = 4.06ADD48 pKa = 4.21VVAVPPALVMGGSGVPNPSAGWVDD72 pKa = 3.67SASRR76 pKa = 11.84LYY78 pKa = 10.33LQPLGFGGEE87 pKa = 3.99AQSFYY92 pKa = 10.76TPEE95 pKa = 4.4LMGQHH100 pKa = 7.19PDD102 pKa = 2.31WSMAEE107 pKa = 4.25GARR110 pKa = 11.84LLTAGIHH117 pKa = 6.41ANLATASAEE126 pKa = 3.85NPVYY130 pKa = 10.95VFGYY134 pKa = 8.55SQSSALSTLTMNQLAADD151 pKa = 4.41GVDD154 pKa = 2.93QDD156 pKa = 4.57LVHH159 pKa = 6.72FVLVGNSANPNGGMLTSFDD178 pKa = 4.26LPLIRR183 pKa = 11.84DD184 pKa = 3.9ISRR187 pKa = 11.84MFEE190 pKa = 3.86MTLGNPTPNLFPTDD204 pKa = 3.91VFTLEE209 pKa = 3.76YY210 pKa = 10.46DD211 pKa = 4.18GYY213 pKa = 11.47ADD215 pKa = 4.69FPRR218 pKa = 11.84YY219 pKa = 8.78PLNPLSTLNALVGLFTQHH237 pKa = 6.41LKK239 pKa = 10.97YY240 pKa = 10.72LGFGYY245 pKa = 10.58DD246 pKa = 4.83DD247 pKa = 4.21LWSSPGVVNPANVVQLDD264 pKa = 4.07TVGDD268 pKa = 3.68LTDD271 pKa = 3.64YY272 pKa = 11.14YY273 pKa = 10.24IIRR276 pKa = 11.84ADD278 pKa = 4.05TLPLLDD284 pKa = 4.63PLRR287 pKa = 11.84LVPVAGRR294 pKa = 11.84PLADD298 pKa = 4.2LLEE301 pKa = 4.91PDD303 pKa = 4.94LRR305 pKa = 11.84ILVNLGYY312 pKa = 11.05GSITDD317 pKa = 3.83GWNSGPADD325 pKa = 3.56VASTIGLFPTEE336 pKa = 3.98LHH338 pKa = 6.5CVAGCSWLDD347 pKa = 3.5LPEE350 pKa = 5.32ALLRR354 pKa = 11.84GAWQGVSDD362 pKa = 5.11FVDD365 pKa = 4.2DD366 pKa = 5.2LLDD369 pKa = 3.74PTTYY373 pKa = 10.96QLADD377 pKa = 5.11PITSPALTTLVNAAINTGVVDD398 pKa = 4.24SAPSSLWEE406 pKa = 4.76LLSAWLDD413 pKa = 3.76TSATPLDD420 pKa = 4.26FGALAGQLGLGGLLDD435 pKa = 3.71WVTTLLASLTGG446 pKa = 3.58

Molecular weight: 47.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A3JM91|A0A1A3JM91_9MYCO Mycobactin synthetase protein B OS=Mycobacterium sp. 1274756.6 OX=1834076 GN=A5643_04955 PE=3 SV=1
MM1 pKa = 7.56LRR3 pKa = 11.84RR4 pKa = 11.84LSALPIDD11 pKa = 4.45KK12 pKa = 10.52FLLALLATVAVATVLPASGAAADD35 pKa = 3.93VLEE38 pKa = 4.62VATKK42 pKa = 10.51AAIALLFWLYY52 pKa = 8.77GTRR55 pKa = 11.84LSPRR59 pKa = 11.84QAWQGLRR66 pKa = 11.84HH67 pKa = 5.48WQLHH71 pKa = 5.52LLVLAITFVVFPVLGLIARR90 pKa = 11.84SLVPTVLTIDD100 pKa = 4.87LYY102 pKa = 10.83TGLLFLCLVPSTVQSSIAFTSIARR126 pKa = 11.84GNVSAAIVSASVSNIVGVVVTPLLVMLLMHH156 pKa = 6.79TGGVPGMDD164 pKa = 3.23PTAIRR169 pKa = 11.84DD170 pKa = 3.6IVVQLLLPFLAGQLMRR186 pKa = 11.84PWVADD191 pKa = 4.48WIARR195 pKa = 11.84HH196 pKa = 5.58AALTRR201 pKa = 11.84VVDD204 pKa = 3.95RR205 pKa = 11.84GSILLVVYY213 pKa = 7.89TAFSIGMTRR222 pKa = 11.84HH223 pKa = 5.07IWSTVAPWQLVAVTVVSALLLALVMGITVVAGQLADD259 pKa = 4.25FSRR262 pKa = 11.84ADD264 pKa = 4.2RR265 pKa = 11.84IVLLFCGSKK274 pKa = 10.38KK275 pKa = 10.6SLASGLPMALVLFPAATVGITMLPLMLFHH304 pKa = 6.9QIQLIVCAVIAARR317 pKa = 11.84LARR320 pKa = 11.84TADD323 pKa = 3.5AAADD327 pKa = 3.73PAA329 pKa = 4.69

Molecular weight: 34.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3346

0

3346

1103034

30

3722

329.7

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.07 ± 0.073

0.762 ± 0.012

6.399 ± 0.031

5.24 ± 0.041

2.92 ± 0.025

8.921 ± 0.034

2.163 ± 0.018

3.937 ± 0.03

1.926 ± 0.028

10.126 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.889 ± 0.018

2.0 ± 0.021

6.042 ± 0.039

2.945 ± 0.021

7.601 ± 0.042

4.748 ± 0.027

6.092 ± 0.024

8.648 ± 0.039

1.468 ± 0.017

2.103 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski