African cassava mosaic Burkina Faso virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8WR14|H8WR14_9GEMI Isoform of H8WR17 Protein V2 OS=African cassava mosaic Burkina Faso virus OX=2560285 GN=AV2 PE=3 SV=1
MM1 pKa = 7.55GSLISMCLFSSKK13 pKa = 10.86EE14 pKa = 3.71NTTAKK19 pKa = 10.49INDD22 pKa = 3.29SSTWYY27 pKa = 8.14PQPGQHH33 pKa = 6.88ISIQTFKK40 pKa = 10.68EE41 pKa = 3.94LNQAPMSSPTSTRR54 pKa = 11.84TEE56 pKa = 3.96TPLNGEE62 pKa = 4.09NSRR65 pKa = 11.84STEE68 pKa = 3.95EE69 pKa = 4.07VLGEE73 pKa = 4.01AARR76 pKa = 11.84TLTTLQQRR84 pKa = 11.84PP85 pKa = 3.55

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8WR35|H8WR35_9GEMI Isoform of H8WR18 Capsid protein OS=African cassava mosaic Burkina Faso virus OX=2560285 GN=AV1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.93IIISTPGSKK16 pKa = 9.66VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 10.29RR28 pKa = 11.84NRR30 pKa = 11.84ATAPTVHH37 pKa = 4.8VTNRR41 pKa = 11.84KK42 pKa = 7.72RR43 pKa = 11.84AWMNRR48 pKa = 11.84PMYY51 pKa = 9.16RR52 pKa = 11.84KK53 pKa = 8.85PMMYY57 pKa = 10.13RR58 pKa = 11.84MYY60 pKa = 10.28RR61 pKa = 11.84SPDD64 pKa = 2.95IPRR67 pKa = 11.84GCEE70 pKa = 4.19GPCKK74 pKa = 10.21VQSFEE79 pKa = 3.87QRR81 pKa = 11.84DD82 pKa = 3.95DD83 pKa = 3.77VKK85 pKa = 11.15HH86 pKa = 6.56LGICKK91 pKa = 10.03VISDD95 pKa = 3.77VTRR98 pKa = 11.84GPGLTHH104 pKa = 6.87RR105 pKa = 11.84VGKK108 pKa = 9.86RR109 pKa = 11.84FCIKK113 pKa = 10.26SIYY116 pKa = 9.81ILGKK120 pKa = 9.09IWMDD124 pKa = 3.64EE125 pKa = 4.09NIKK128 pKa = 10.08KK129 pKa = 9.99QNHH132 pKa = 4.98TNNVMFYY139 pKa = 10.26LLRR142 pKa = 11.84DD143 pKa = 3.45RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 11.37GNTPQDD153 pKa = 3.26FGQIFNMFDD162 pKa = 3.66NEE164 pKa = 4.15PSTATIKK171 pKa = 10.92NDD173 pKa = 2.93LRR175 pKa = 11.84DD176 pKa = 3.48RR177 pKa = 11.84FQVLRR182 pKa = 11.84KK183 pKa = 8.78FHH185 pKa = 6.12ATVIGGPSGMKK196 pKa = 9.5EE197 pKa = 3.54QALVKK202 pKa = 10.21RR203 pKa = 11.84FYY205 pKa = 10.9RR206 pKa = 11.84LNHH209 pKa = 5.75HH210 pKa = 5.51VTYY213 pKa = 10.8NHH215 pKa = 6.01QEE217 pKa = 3.51AGKK220 pKa = 10.41YY221 pKa = 8.39EE222 pKa = 4.09NHH224 pKa = 6.52TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.63FYY253 pKa = 11.33DD254 pKa = 3.78SIGNN258 pKa = 3.69

Molecular weight: 30.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

1638

85

359

204.8

23.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.762 ± 0.706

1.77 ± 0.213

5.311 ± 0.7

4.64 ± 0.483

4.396 ± 0.334

5.25 ± 0.3

3.846 ± 0.534

6.166 ± 0.681

5.8 ± 0.728

6.716 ± 0.459

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.349

5.739 ± 0.751

5.739 ± 0.413

4.945 ± 0.34

6.716 ± 0.922

8.73 ± 1.088

6.166 ± 0.712

5.739 ± 1.0

1.16 ± 0.217

4.151 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski