Lysobacter aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A508AM07|A0A508AM07_9GAMM Type I secretion system permease/ATPase OS=Lysobacter aestuarii OX=1706195 GN=FKV25_06390 PE=4 SV=1
MM1 pKa = 7.55SSHH4 pKa = 5.66TKK6 pKa = 9.74IVASKK11 pKa = 9.48FATRR15 pKa = 11.84GMPSVLLMAMFAAMGMARR33 pKa = 11.84AEE35 pKa = 4.16AGEE38 pKa = 4.1VSGSITLTSDD48 pKa = 3.21YY49 pKa = 10.35LFRR52 pKa = 11.84GITQTNEE59 pKa = 3.26EE60 pKa = 4.41PALQGGIEE68 pKa = 4.05YY69 pKa = 10.4AADD72 pKa = 3.14SGFYY76 pKa = 10.25AGAWGSSISWLSDD89 pKa = 2.95SDD91 pKa = 4.2PDD93 pKa = 3.37ISSQVEE99 pKa = 3.17IDD101 pKa = 3.69GYY103 pKa = 10.8LGYY106 pKa = 10.6RR107 pKa = 11.84GDD109 pKa = 4.18FGDD112 pKa = 3.89SGVGYY117 pKa = 10.44DD118 pKa = 4.45VGAIYY123 pKa = 10.48YY124 pKa = 7.44WYY126 pKa = 9.76PGDD129 pKa = 3.81YY130 pKa = 10.47PSGFNSADD138 pKa = 3.24TTEE141 pKa = 4.35VYY143 pKa = 10.53FGVGWSFLSAKK154 pKa = 10.03YY155 pKa = 10.42SYY157 pKa = 11.43AVTDD161 pKa = 4.04LFGIPDD167 pKa = 3.84SDD169 pKa = 3.75GSSALDD175 pKa = 4.47LGASWEE181 pKa = 4.25VAPGWSLDD189 pKa = 3.63AAVGKK194 pKa = 9.86QWVEE198 pKa = 3.81NVDD201 pKa = 3.73GGDD204 pKa = 3.4YY205 pKa = 10.68AYY207 pKa = 9.76WKK209 pKa = 10.39VGAGKK214 pKa = 10.54SFDD217 pKa = 3.63NGFDD221 pKa = 3.0IAVAYY226 pKa = 10.39NDD228 pKa = 4.03NDD230 pKa = 4.82LIGPDD235 pKa = 3.89EE236 pKa = 4.25TWTFAVTKK244 pKa = 10.51SFF246 pKa = 3.8

Molecular weight: 26.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A508A9E4|A0A508A9E4_9GAMM ATP-binding protein OS=Lysobacter aestuarii OX=1706195 GN=FKV25_06580 PE=4 SV=1
MM1 pKa = 7.52PARR4 pKa = 11.84RR5 pKa = 11.84PRR7 pKa = 11.84ARR9 pKa = 11.84PRR11 pKa = 11.84SRR13 pKa = 11.84GRR15 pKa = 11.84KK16 pKa = 8.33GRR18 pKa = 11.84ARR20 pKa = 11.84PPSPPLPRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84SSRR34 pKa = 11.84PGHH37 pKa = 5.22QPFLRR42 pKa = 11.84SPTRR46 pKa = 11.84HH47 pKa = 4.46TRR49 pKa = 11.84HH50 pKa = 5.63RR51 pKa = 11.84RR52 pKa = 11.84MPRR55 pKa = 11.84RR56 pKa = 11.84PLRR59 pKa = 11.84RR60 pKa = 11.84KK61 pKa = 9.83APSRR65 pKa = 11.84SIAVAGPTPIARR77 pKa = 3.96

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3124

0

3124

1031292

30

2907

330.1

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.268 ± 0.057

0.839 ± 0.013

6.257 ± 0.038

5.605 ± 0.044

3.31 ± 0.03

8.951 ± 0.04

2.301 ± 0.024

3.891 ± 0.029

2.367 ± 0.035

10.583 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.021

2.281 ± 0.028

5.586 ± 0.035

3.257 ± 0.026

8.061 ± 0.052

5.066 ± 0.029

4.74 ± 0.045

7.595 ± 0.042

1.555 ± 0.023

2.25 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski