Infectious pancreatic necrosis virus (strain Sp) (IPNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Aquabirnavirus; Infectious pancreatic necrosis virus

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q703G8|Q703G8_IPNVS Isoform of P22174 Protein VP1 OS=Infectious pancreatic necrosis virus (strain Sp) OX=11005 GN=VP1 PE=4 SV=1
MM1 pKa = 6.89NTNKK5 pKa = 8.97ATATYY10 pKa = 9.13LKK12 pKa = 10.5SIMLPEE18 pKa = 4.74TGPASIPDD26 pKa = 5.16DD27 pKa = 3.12ITEE30 pKa = 3.99RR31 pKa = 11.84HH32 pKa = 5.89ILKK35 pKa = 10.07QEE37 pKa = 3.84TSSYY41 pKa = 9.6NLEE44 pKa = 4.05VSEE47 pKa = 4.58SGSGVLVCFPGAPGSRR63 pKa = 11.84IGAHH67 pKa = 5.45YY68 pKa = 9.97RR69 pKa = 11.84WNANQTGLEE78 pKa = 3.87FDD80 pKa = 3.19QWLEE84 pKa = 3.86TSQDD88 pKa = 3.07LKK90 pKa = 11.21KK91 pKa = 10.75AFNYY95 pKa = 9.81GRR97 pKa = 11.84LISRR101 pKa = 11.84KK102 pKa = 9.31YY103 pKa = 10.36DD104 pKa = 3.38IQSSTLPAGLYY115 pKa = 10.34ALNGTLNAATFEE127 pKa = 4.46GSLSEE132 pKa = 4.62VEE134 pKa = 4.23SLTYY138 pKa = 10.53NSLMSLTTNPQDD150 pKa = 3.31KK151 pKa = 10.9VNNQLVTKK159 pKa = 10.21GVTVLNLPTGFDD171 pKa = 3.17KK172 pKa = 10.97PYY174 pKa = 10.8VRR176 pKa = 11.84LEE178 pKa = 4.22DD179 pKa = 3.65EE180 pKa = 4.74TPQGLQSMNGAKK192 pKa = 8.9MRR194 pKa = 11.84CTAAIAPRR202 pKa = 11.84RR203 pKa = 11.84YY204 pKa = 10.24EE205 pKa = 3.63IDD207 pKa = 3.84LPSQRR212 pKa = 11.84LPPVPATGTLTTLYY226 pKa = 9.76EE227 pKa = 4.36GNADD231 pKa = 3.14IVNSTTVTGDD241 pKa = 3.01INFSLAEE248 pKa = 3.99QPADD252 pKa = 3.32EE253 pKa = 4.7TKK255 pKa = 10.68FDD257 pKa = 3.87FQLDD261 pKa = 3.72FMGLDD266 pKa = 3.12NDD268 pKa = 4.11VPVVTVVSSVLATNDD283 pKa = 2.83NYY285 pKa = 11.11RR286 pKa = 11.84GVSAKK291 pKa = 7.47MTQSIPTEE299 pKa = 4.45NITKK303 pKa = 10.03PITRR307 pKa = 11.84VKK309 pKa = 10.53LSYY312 pKa = 10.45KK313 pKa = 10.06INQQTAIGNVATLGTMGPASVSFSSGNGNVPGVLRR348 pKa = 11.84PITLVAYY355 pKa = 9.88EE356 pKa = 4.56KK357 pKa = 7.77MTPLSILTVAGVSNYY372 pKa = 9.4EE373 pKa = 4.44LIPNPEE379 pKa = 4.27LLKK382 pKa = 11.42NMVTRR387 pKa = 11.84YY388 pKa = 9.74GKK390 pKa = 10.45YY391 pKa = 10.22DD392 pKa = 3.71PEE394 pKa = 4.29GLNYY398 pKa = 10.95AKK400 pKa = 10.06MILSHH405 pKa = 7.46RR406 pKa = 11.84EE407 pKa = 3.56EE408 pKa = 4.78LDD410 pKa = 2.89IRR412 pKa = 11.84TVWRR416 pKa = 11.84TEE418 pKa = 3.79EE419 pKa = 3.98YY420 pKa = 10.51KK421 pKa = 10.88EE422 pKa = 3.75RR423 pKa = 11.84TRR425 pKa = 11.84VFNEE429 pKa = 3.48ITDD432 pKa = 4.37FSSDD436 pKa = 3.78LPTSKK441 pKa = 10.5AWGWRR446 pKa = 11.84DD447 pKa = 2.97IVRR450 pKa = 11.84GIRR453 pKa = 11.84KK454 pKa = 8.54VAAPVLSTLFPMAAPLIGMADD475 pKa = 3.49QFIGDD480 pKa = 3.9LTKK483 pKa = 10.75TNAAGGRR490 pKa = 11.84YY491 pKa = 9.37HH492 pKa = 8.3SMAAGGRR499 pKa = 11.84YY500 pKa = 9.4KK501 pKa = 10.73DD502 pKa = 4.16VLEE505 pKa = 4.56SWASGGPDD513 pKa = 3.01GKK515 pKa = 10.61FSRR518 pKa = 11.84ALKK521 pKa = 10.49NRR523 pKa = 11.84LEE525 pKa = 4.14SANYY529 pKa = 10.01EE530 pKa = 4.32EE531 pKa = 5.78VEE533 pKa = 4.58LPPPSKK539 pKa = 10.69GVIVPVVHH547 pKa = 5.83TVKK550 pKa = 10.46SAPGEE555 pKa = 4.17AFGSLAIIIPGEE567 pKa = 4.1YY568 pKa = 9.88PEE570 pKa = 5.82LLDD573 pKa = 5.53ANQQVLSHH581 pKa = 6.79FANDD585 pKa = 3.56TGSVWGIGEE594 pKa = 5.12DD595 pKa = 4.07IPFEE599 pKa = 4.38GDD601 pKa = 3.29NMCYY605 pKa = 9.05TALPLKK611 pKa = 9.86EE612 pKa = 3.89IKK614 pKa = 10.42RR615 pKa = 11.84NGNIVVEE622 pKa = 4.86KK623 pKa = 10.34IFAGPIMGPSAQLGLSLLVNDD644 pKa = 5.1IEE646 pKa = 5.31DD647 pKa = 3.96GVPRR651 pKa = 11.84MVFTGEE657 pKa = 3.79IADD660 pKa = 4.34DD661 pKa = 4.22EE662 pKa = 4.48EE663 pKa = 4.84TIIPICGVDD672 pKa = 3.33IKK674 pKa = 11.13AIAAHH679 pKa = 5.51EE680 pKa = 4.08QGLPLIGNQPGVDD693 pKa = 3.08EE694 pKa = 4.43EE695 pKa = 4.5VRR697 pKa = 11.84NTSLAAHH704 pKa = 7.67LIQTGTLPVQRR715 pKa = 11.84AKK717 pKa = 11.05GSNKK721 pKa = 9.2RR722 pKa = 11.84IKK724 pKa = 10.75YY725 pKa = 9.78LGEE728 pKa = 3.98LMASNASGMDD738 pKa = 3.43EE739 pKa = 3.92EE740 pKa = 5.05LQRR743 pKa = 11.84LLNATMARR751 pKa = 11.84AKK753 pKa = 9.75EE754 pKa = 4.04VQDD757 pKa = 3.54AEE759 pKa = 4.25IYY761 pKa = 10.62KK762 pKa = 10.24LLKK765 pKa = 10.5LMAWTRR771 pKa = 11.84KK772 pKa = 9.59NDD774 pKa = 3.94LTDD777 pKa = 5.05HH778 pKa = 5.96MYY780 pKa = 10.29EE781 pKa = 3.96WSKK784 pKa = 11.34EE785 pKa = 4.01DD786 pKa = 3.59PDD788 pKa = 3.72ALKK791 pKa = 10.49FGKK794 pKa = 10.38LISTPPKK801 pKa = 10.27HH802 pKa = 6.15PEE804 pKa = 3.87KK805 pKa = 10.57PKK807 pKa = 11.16GPDD810 pKa = 2.87QHH812 pKa = 6.6HH813 pKa = 6.05AQEE816 pKa = 4.23ARR818 pKa = 11.84ATRR821 pKa = 11.84ISLDD825 pKa = 3.32AVRR828 pKa = 11.84AGADD832 pKa = 3.26FATPEE837 pKa = 3.85WVALNNYY844 pKa = 8.8RR845 pKa = 11.84GPSPGQFKK853 pKa = 11.0YY854 pKa = 11.19YY855 pKa = 10.24LITGRR860 pKa = 11.84EE861 pKa = 4.09PEE863 pKa = 4.93PGDD866 pKa = 3.71EE867 pKa = 4.18YY868 pKa = 11.25EE869 pKa = 5.85DD870 pKa = 4.6YY871 pKa = 10.8IKK873 pKa = 10.78QPIVKK878 pKa = 8.42PTDD881 pKa = 3.21MNKK884 pKa = 9.11IRR886 pKa = 11.84RR887 pKa = 11.84LANSVYY893 pKa = 10.06GLPHH897 pKa = 6.09QEE899 pKa = 3.77PAPEE903 pKa = 3.88EE904 pKa = 4.51FYY906 pKa = 11.33DD907 pKa = 3.79AVAAVFAQNGGRR919 pKa = 11.84GPDD922 pKa = 3.35QDD924 pKa = 3.31QMQDD928 pKa = 3.13LRR930 pKa = 11.84EE931 pKa = 4.26LARR934 pKa = 11.84QMKK937 pKa = 9.58RR938 pKa = 11.84RR939 pKa = 11.84PRR941 pKa = 11.84NADD944 pKa = 2.72APRR947 pKa = 11.84RR948 pKa = 11.84TRR950 pKa = 11.84APAEE954 pKa = 3.76PAPPGRR960 pKa = 11.84SRR962 pKa = 11.84FTPSGDD968 pKa = 3.33NAEE971 pKa = 4.26VV972 pKa = 3.07

Molecular weight:
106.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|Q703G9|POLS_IPNVS Structural polyprotein OS=Infectious pancreatic necrosis virus (strain Sp) OX=11005 PE=1 SV=1
MM1 pKa = 7.67SDD3 pKa = 4.08IFNSPQNKK11 pKa = 9.77ASILNALMKK20 pKa = 9.7STQGDD25 pKa = 3.83VEE27 pKa = 4.64DD28 pKa = 3.71VLIPKK33 pKa = 10.06RR34 pKa = 11.84FRR36 pKa = 11.84PAKK39 pKa = 10.35DD40 pKa = 3.61PLDD43 pKa = 4.22SPQAAAAFLKK53 pKa = 8.53EE54 pKa = 3.81HH55 pKa = 7.62KK56 pKa = 10.37YY57 pKa = 10.63RR58 pKa = 11.84ILRR61 pKa = 11.84PRR63 pKa = 11.84AIPTMVEE70 pKa = 3.76IEE72 pKa = 3.98TDD74 pKa = 3.16AALPRR79 pKa = 11.84LAAMVDD85 pKa = 3.51DD86 pKa = 5.06GKK88 pKa = 11.05LKK90 pKa = 10.86EE91 pKa = 4.23MVNVPEE97 pKa = 4.55GTTAFYY103 pKa = 10.05PKK105 pKa = 9.82YY106 pKa = 10.15YY107 pKa = 9.64PFHH110 pKa = 7.17RR111 pKa = 11.84PDD113 pKa = 4.02HH114 pKa = 7.24DD115 pKa = 4.14DD116 pKa = 3.18VGTFGAPDD124 pKa = 3.15ITLLKK129 pKa = 10.52QLTFFLLEE137 pKa = 3.99NDD139 pKa = 4.02FPTGPEE145 pKa = 3.55TLRR148 pKa = 11.84QVRR151 pKa = 11.84EE152 pKa = 4.48AIATLQYY159 pKa = 11.35GSGSYY164 pKa = 10.01SGQLNRR170 pKa = 11.84LLAMKK175 pKa = 10.44GVATGRR181 pKa = 11.84NPNKK185 pKa = 9.57TPLAVGYY192 pKa = 8.5TNEE195 pKa = 3.64QMARR199 pKa = 11.84LMEE202 pKa = 4.05QTLPINPPKK211 pKa = 10.96NEE213 pKa = 4.26DD214 pKa = 3.58PDD216 pKa = 4.95LRR218 pKa = 11.84WAPSWLIQYY227 pKa = 8.7TGDD230 pKa = 3.31ASTDD234 pKa = 3.1KK235 pKa = 11.06SYY237 pKa = 11.24LPHH240 pKa = 5.43VTAKK244 pKa = 10.48SSAGLPYY251 pKa = 9.88IGKK254 pKa = 8.15TKK256 pKa = 10.95GDD258 pKa = 3.64TTAEE262 pKa = 4.03ALVLADD268 pKa = 4.42SFIRR272 pKa = 11.84DD273 pKa = 3.54LGKK276 pKa = 10.31AATSADD282 pKa = 3.33PGAAAKK288 pKa = 10.14KK289 pKa = 9.51VLSDD293 pKa = 3.77FWYY296 pKa = 10.37LSCGLLFPKK305 pKa = 10.51GEE307 pKa = 4.59RR308 pKa = 11.84YY309 pKa = 7.04TQKK312 pKa = 11.01DD313 pKa = 2.99WDD315 pKa = 4.02LKK317 pKa = 9.48TRR319 pKa = 11.84NIWSAPYY326 pKa = 7.69PTHH329 pKa = 7.06LLLSMVSSPVMDD341 pKa = 3.73EE342 pKa = 4.1SKK344 pKa = 11.34LNITNTQTPSLYY356 pKa = 10.6GFSPFHH362 pKa = 6.53GGINRR367 pKa = 11.84IMTIIRR373 pKa = 11.84EE374 pKa = 4.27HH375 pKa = 7.06LDD377 pKa = 3.36QEE379 pKa = 4.18QDD381 pKa = 3.18LVMIYY386 pKa = 10.95ADD388 pKa = 4.6NIYY391 pKa = 10.23ILQDD395 pKa = 3.45NTWYY399 pKa = 10.96SIDD402 pKa = 4.01LEE404 pKa = 4.4KK405 pKa = 11.54GEE407 pKa = 4.74ANCTPQHH414 pKa = 5.47MQAMMYY420 pKa = 10.23YY421 pKa = 9.91RR422 pKa = 11.84LTRR425 pKa = 11.84EE426 pKa = 3.81WTNEE430 pKa = 3.98DD431 pKa = 2.94GSPRR435 pKa = 11.84YY436 pKa = 9.89NPTWATFAMYY446 pKa = 9.92VGPSMVVDD454 pKa = 4.21STCLLMNLQLKK465 pKa = 6.82TTGQGSGNAFTFLNNHH481 pKa = 6.13LMSTIVVAEE490 pKa = 3.65WHH492 pKa = 6.25KK493 pKa = 11.05AGRR496 pKa = 11.84PNPMSKK502 pKa = 10.45EE503 pKa = 3.95FMDD506 pKa = 5.28LEE508 pKa = 4.64AKK510 pKa = 9.88TGINFKK516 pKa = 10.2IEE518 pKa = 4.2RR519 pKa = 11.84EE520 pKa = 4.18LKK522 pKa = 9.94DD523 pKa = 3.35LRR525 pKa = 11.84SIIMEE530 pKa = 4.43AVDD533 pKa = 3.58TAPLDD538 pKa = 4.01GYY540 pKa = 11.04LADD543 pKa = 5.56GSDD546 pKa = 3.95LPPRR550 pKa = 11.84VPGKK554 pKa = 10.37AVEE557 pKa = 4.12LDD559 pKa = 3.63LLGWSAVYY567 pKa = 10.23SRR569 pKa = 11.84QLEE572 pKa = 4.22MFVPVLEE579 pKa = 4.05NEE581 pKa = 4.02RR582 pKa = 11.84LIASVAYY589 pKa = 9.32PKK591 pKa = 10.89GLEE594 pKa = 4.09NKK596 pKa = 10.05SLARR600 pKa = 11.84KK601 pKa = 8.88PGAEE605 pKa = 3.3IAYY608 pKa = 9.8QIVRR612 pKa = 11.84YY613 pKa = 8.57EE614 pKa = 4.38AIRR617 pKa = 11.84LIGGWNNPLIEE628 pKa = 4.13TAAKK632 pKa = 10.34HH633 pKa = 5.12MSLDD637 pKa = 3.04KK638 pKa = 10.75RR639 pKa = 11.84KK640 pKa = 8.93RR641 pKa = 11.84LEE643 pKa = 3.9VKK645 pKa = 10.72GIDD648 pKa = 3.35VTGFLDD654 pKa = 3.56DD655 pKa = 3.97WNTMSEE661 pKa = 4.18FGGDD665 pKa = 3.55LEE667 pKa = 5.43GISLTAPLTNQTLLDD682 pKa = 3.63INTPEE687 pKa = 4.05TEE689 pKa = 4.02FDD691 pKa = 3.79VKK693 pKa = 10.77DD694 pKa = 3.84RR695 pKa = 11.84PPTPRR700 pKa = 11.84SPGKK704 pKa = 8.25TLAEE708 pKa = 3.92VTAAITSGTYY718 pKa = 9.95KK719 pKa = 10.54DD720 pKa = 3.78PKK722 pKa = 8.57SAVWRR727 pKa = 11.84LLDD730 pKa = 3.27QRR732 pKa = 11.84TKK734 pKa = 11.18LRR736 pKa = 11.84VSTLRR741 pKa = 11.84DD742 pKa = 3.04HH743 pKa = 7.15AHH745 pKa = 6.38ALKK748 pKa = 9.97PAASTSDD755 pKa = 3.35FWGDD759 pKa = 3.15ATEE762 pKa = 4.83EE763 pKa = 4.01LAEE766 pKa = 3.99QQQLLMKK773 pKa = 10.59ANNLLKK779 pKa = 10.81SSLTEE784 pKa = 3.57ARR786 pKa = 11.84EE787 pKa = 3.98ALEE790 pKa = 4.35TVQSDD795 pKa = 3.89KK796 pKa = 10.91IISGKK801 pKa = 8.33TSPEE805 pKa = 4.1KK806 pKa = 11.11NPGTAANPVVAYY818 pKa = 10.43GEE820 pKa = 4.16FSEE823 pKa = 5.61KK824 pKa = 10.33IPLTPTQKK832 pKa = 10.69KK833 pKa = 7.32NAKK836 pKa = 9.43RR837 pKa = 11.84RR838 pKa = 11.84EE839 pKa = 3.99KK840 pKa = 10.41QRR842 pKa = 11.84RR843 pKa = 11.84NN844 pKa = 3.14

Molecular weight:
94.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

2660

844

972

886.7

98.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.571 ± 0.119

0.376 ± 0.015

6.053 ± 0.22

6.316 ± 0.204

2.857 ± 0.078

6.617 ± 0.47

1.466 ± 0.055

5.0 ± 0.195

6.053 ± 0.396

9.812 ± 0.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.139

4.85 ± 0.221

7.03 ± 0.118

3.534 ± 0.037

5.113 ± 0.123

6.203 ± 0.032

7.368 ± 0.249

5.15 ± 0.392

1.429 ± 0.172

3.308 ± 0.05

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski