Actinopolyspora mzabensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinopolysporales; Actinopolysporaceae; Actinopolyspora

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4438 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9BHW2|A0A1G9BHW2_9ACTN Uncharacterized protein OS=Actinopolyspora mzabensis OX=995066 GN=SAMN04487820_107165 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84KK3 pKa = 9.27SVLSNCCSAVLAAALLPMLAPAASAVTDD31 pKa = 3.46RR32 pKa = 11.84ADD34 pKa = 3.36PVEE37 pKa = 4.29YY38 pKa = 10.54VALGDD43 pKa = 4.14SYY45 pKa = 11.99ASGVGIGNYY54 pKa = 8.14TPEE57 pKa = 5.27SGDD60 pKa = 3.84CLRR63 pKa = 11.84SPQAYY68 pKa = 7.67PRR70 pKa = 11.84LVADD74 pKa = 4.85RR75 pKa = 11.84GAVDD79 pKa = 3.6SLDD82 pKa = 3.6FAACAGAEE90 pKa = 4.26TPDD93 pKa = 3.57VLNEE97 pKa = 3.92QVTALSSSTDD107 pKa = 3.33VVTVSIGGNDD117 pKa = 3.23AGFVDD122 pKa = 6.53VITTCNTGGDD132 pKa = 3.9QTCDD136 pKa = 3.45DD137 pKa = 4.48AVTEE141 pKa = 4.27AEE143 pKa = 4.5NYY145 pKa = 7.52ATQQLPAEE153 pKa = 4.2LAEE156 pKa = 4.49TYY158 pKa = 10.65DD159 pKa = 4.52AIGAAAPQARR169 pKa = 11.84VAVVGYY175 pKa = 9.27PRR177 pKa = 11.84LFEE180 pKa = 5.33LGSCLSLLSEE190 pKa = 4.58FEE192 pKa = 4.02RR193 pKa = 11.84GRR195 pKa = 11.84LNDD198 pKa = 3.89AADD201 pKa = 3.78TLDD204 pKa = 4.12SVIAEE209 pKa = 4.01QAAAAGFSFLDD220 pKa = 3.39VRR222 pKa = 11.84DD223 pKa = 4.28DD224 pKa = 4.05FSGHH228 pKa = 5.46GVCGAQPWINGLSYY242 pKa = 9.8PAEE245 pKa = 4.12EE246 pKa = 4.76SFHH249 pKa = 6.94PDD251 pKa = 2.82AAGHH255 pKa = 5.31EE256 pKa = 4.44QGFLPDD262 pKa = 3.25VSGFLATQSPLASS275 pKa = 3.45

Molecular weight: 28.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9AJX2|A0A1G9AJX2_9ACTN Uncharacterized protein OS=Actinopolyspora mzabensis OX=995066 GN=SAMN04487820_10693 PE=4 SV=1
MM1 pKa = 7.47SKK3 pKa = 10.58GKK5 pKa = 8.48RR6 pKa = 11.84TYY8 pKa = 10.26QPNNRR13 pKa = 11.84KK14 pKa = 9.12RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 6.07GFRR22 pKa = 11.84QRR24 pKa = 11.84MHH26 pKa = 5.44TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84KK43 pKa = 9.15RR44 pKa = 11.84LTAA47 pKa = 4.25

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4438

0

4438

1404463

39

4364

316.5

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.694 ± 0.051

0.827 ± 0.011

5.775 ± 0.031

6.871 ± 0.047

2.782 ± 0.023

9.246 ± 0.04

2.371 ± 0.02

3.261 ± 0.028

1.769 ± 0.022

10.332 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.835 ± 0.015

2.17 ± 0.019

5.665 ± 0.03

2.838 ± 0.024

8.437 ± 0.039

6.161 ± 0.033

6.067 ± 0.026

8.453 ± 0.036

1.476 ± 0.016

1.97 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski