Raspberry leaf blotch emaravirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8K9Y7|F8K9Y7_9VIRU Replicase OS=Raspberry leaf blotch emaravirus OX=1980431 GN=RDRP PE=4 SV=1
MM1 pKa = 7.99SEE3 pKa = 3.48IGITIEE9 pKa = 6.14DD10 pKa = 3.52ILEE13 pKa = 4.18NLWIEE18 pKa = 4.16LFNAISEE25 pKa = 4.36YY26 pKa = 11.27AKK28 pKa = 10.72VGEE31 pKa = 4.14YY32 pKa = 10.81DD33 pKa = 4.37DD34 pKa = 6.23LINLKK39 pKa = 10.73DD40 pKa = 3.66EE41 pKa = 4.67LLDD44 pKa = 4.01GGEE47 pKa = 4.53AEE49 pKa = 4.78SFSKK53 pKa = 10.73PGLKK57 pKa = 10.49VIFKK61 pKa = 9.88FNKK64 pKa = 9.49LAFICTQIVDD74 pKa = 4.06YY75 pKa = 11.57DD76 pKa = 3.88NILYY80 pKa = 10.42RR81 pKa = 11.84KK82 pKa = 9.25FKK84 pKa = 10.75NHH86 pKa = 6.3FDD88 pKa = 3.27KK89 pKa = 11.36FGIKK93 pKa = 8.88MLNKK97 pKa = 10.25DD98 pKa = 3.06YY99 pKa = 10.15TLRR102 pKa = 11.84YY103 pKa = 9.29RR104 pKa = 11.84NPIGLARR111 pKa = 11.84EE112 pKa = 4.16IFNHH116 pKa = 5.56VNFKK120 pKa = 10.05MDD122 pKa = 3.93LSDD125 pKa = 3.45VEE127 pKa = 4.3YY128 pKa = 10.4WNGFFITITGEE139 pKa = 3.95LNKK142 pKa = 9.69TKK144 pKa = 10.57SIMEE148 pKa = 3.76GDD150 pKa = 3.71YY151 pKa = 10.59IVRR154 pKa = 11.84CEE156 pKa = 3.89NQLKK160 pKa = 10.17IIHH163 pKa = 6.64KK164 pKa = 9.71LDD166 pKa = 4.42GIIRR170 pKa = 11.84TIQKK174 pKa = 9.94KK175 pKa = 9.73SKK177 pKa = 9.55EE178 pKa = 4.15YY179 pKa = 10.67DD180 pKa = 2.94SSTIKK185 pKa = 10.85NYY187 pKa = 9.84IDD189 pKa = 3.06FTMYY193 pKa = 11.0LDD195 pKa = 3.97DD196 pKa = 4.28CSYY199 pKa = 11.28VVSSSQVRR207 pKa = 11.84RR208 pKa = 11.84NDD210 pKa = 3.09EE211 pKa = 4.16DD212 pKa = 3.76CAEE215 pKa = 4.16TVIDD219 pKa = 4.3EE220 pKa = 4.62NQDD223 pKa = 3.31KK224 pKa = 11.33LNIAEE229 pKa = 4.3SDD231 pKa = 3.48ATT233 pKa = 3.76

Molecular weight: 27.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8K9Y9|F8K9Y9_9VIRU Nucleocapsid OS=Raspberry leaf blotch emaravirus OX=1980431 GN=NC PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.48IMVQLILLCILLQFIASEE20 pKa = 4.39SSEE23 pKa = 4.0SRR25 pKa = 11.84PDD27 pKa = 3.51SEE29 pKa = 4.95YY30 pKa = 11.34KK31 pKa = 10.41EE32 pKa = 4.22ADD34 pKa = 3.79EE35 pKa = 5.6NIHH38 pKa = 6.8DD39 pKa = 4.44CSLKK43 pKa = 10.38VVRR46 pKa = 11.84LDD48 pKa = 5.04GIQLACRR55 pKa = 11.84GKK57 pKa = 9.24ILCWFSTNAVAYY69 pKa = 10.81NDD71 pKa = 4.21TCWKK75 pKa = 8.65LTEE78 pKa = 4.32TSTHH82 pKa = 5.35FHH84 pKa = 6.87CCNKK88 pKa = 10.15YY89 pKa = 9.44EE90 pKa = 3.95AVIYY94 pKa = 10.09KK95 pKa = 10.24ISDD98 pKa = 3.18EE99 pKa = 4.35SRR101 pKa = 11.84NICYY105 pKa = 10.3DD106 pKa = 3.44VFSHH110 pKa = 5.78SWCVFLSNLYY120 pKa = 10.49HH121 pKa = 7.11FILAILIWIIMALARR136 pKa = 11.84VPFLYY141 pKa = 10.07IFKK144 pKa = 10.34IIDD147 pKa = 4.57LISGKK152 pKa = 8.99ILKK155 pKa = 9.75KK156 pKa = 10.41KK157 pKa = 10.0KK158 pKa = 10.24SCVKK162 pKa = 10.86CEE164 pKa = 3.65DD165 pKa = 3.41KK166 pKa = 11.31YY167 pKa = 11.65AFFHH171 pKa = 6.82IDD173 pKa = 3.26CSSSTRR179 pKa = 11.84KK180 pKa = 9.6RR181 pKa = 11.84VDD183 pKa = 3.17YY184 pKa = 9.05NTVYY188 pKa = 8.61YY189 pKa = 9.55TIVILVIFLVPVFASGEE206 pKa = 4.05NLKK209 pKa = 11.32DD210 pKa = 3.41NDD212 pKa = 4.53SKK214 pKa = 11.65FNTYY218 pKa = 10.32DD219 pKa = 2.96HH220 pKa = 6.74SGYY223 pKa = 9.38TEE225 pKa = 3.97FVVKK229 pKa = 10.56DD230 pKa = 3.54VEE232 pKa = 4.38HH233 pKa = 6.58EE234 pKa = 4.21VNEE237 pKa = 4.88FYY239 pKa = 10.85TKK241 pKa = 10.13TNHH244 pKa = 5.9IKK246 pKa = 8.7ITIEE250 pKa = 3.55KK251 pKa = 10.08SYY253 pKa = 10.8ISYY256 pKa = 10.25VLSYY260 pKa = 11.02SHH262 pKa = 7.65DD263 pKa = 3.79VLKK266 pKa = 9.78KK267 pKa = 7.76TNDD270 pKa = 3.33VITSLEE276 pKa = 4.03YY277 pKa = 10.97SCDD280 pKa = 3.85SEE282 pKa = 4.5EE283 pKa = 3.72KK284 pKa = 10.33CYY286 pKa = 10.75RR287 pKa = 11.84SLKK290 pKa = 9.63GDD292 pKa = 2.94NRR294 pKa = 11.84TFRR297 pKa = 11.84SLKK300 pKa = 10.0KK301 pKa = 10.01NHH303 pKa = 7.3DD304 pKa = 3.76GLSCIFSNAIVCMACSFHH322 pKa = 7.4YY323 pKa = 10.5EE324 pKa = 4.32LFAEE328 pKa = 4.98VYY330 pKa = 9.28TVTEE334 pKa = 4.29HH335 pKa = 7.18KK336 pKa = 10.36PIILMSVEE344 pKa = 3.94INNSEE349 pKa = 4.41KK350 pKa = 11.22YY351 pKa = 10.72NITLSSYY358 pKa = 11.2NDD360 pKa = 3.46YY361 pKa = 11.16VDD363 pKa = 3.35EE364 pKa = 4.29NFYY367 pKa = 10.63IRR369 pKa = 11.84SLNAVDD375 pKa = 4.21FTDD378 pKa = 6.09DD379 pKa = 2.96IVLIKK384 pKa = 10.89GLVAYY389 pKa = 9.31KK390 pKa = 10.7GKK392 pKa = 9.93ICTVPSLDD400 pKa = 4.65CFGSHH405 pKa = 6.01IKK407 pKa = 10.21KK408 pKa = 10.32DD409 pKa = 3.77NKK411 pKa = 9.49ILAMYY416 pKa = 10.21NPIITDD422 pKa = 3.32NNHH425 pKa = 6.05YY426 pKa = 7.91DD427 pKa = 3.48TTVNLVKK434 pKa = 10.25CTEE437 pKa = 3.98NEE439 pKa = 3.96NLDD442 pKa = 4.05LLRR445 pKa = 11.84LSKK448 pKa = 10.57IGLIVDD454 pKa = 3.66KK455 pKa = 11.1DD456 pKa = 3.62RR457 pKa = 11.84VIEE460 pKa = 4.31DD461 pKa = 3.17RR462 pKa = 11.84SFGYY466 pKa = 10.25FSVGVRR472 pKa = 11.84NKK474 pKa = 9.67MLLDD478 pKa = 3.59NKK480 pKa = 10.04ICEE483 pKa = 3.86KK484 pKa = 10.73DD485 pKa = 3.42AIVRR489 pKa = 11.84SIEE492 pKa = 4.17ANGCYY497 pKa = 9.59NCKK500 pKa = 10.25FGFNVKK506 pKa = 9.73INYY509 pKa = 9.27KK510 pKa = 10.32LYY512 pKa = 10.16SEE514 pKa = 4.69CGKK517 pKa = 9.94IEE519 pKa = 4.56CKK521 pKa = 9.8TPIYY525 pKa = 10.39KK526 pKa = 10.35SYY528 pKa = 10.04TYY530 pKa = 11.03VSNGTTANIKK540 pKa = 10.32LYY542 pKa = 10.88SDD544 pKa = 5.11DD545 pKa = 4.25YY546 pKa = 10.22LTKK549 pKa = 9.29ITCNGKK555 pKa = 9.15EE556 pKa = 3.99VQIKK560 pKa = 10.48LDD562 pKa = 3.51SLNIDD567 pKa = 3.4SYY569 pKa = 9.92YY570 pKa = 10.89HH571 pKa = 6.0NNAYY575 pKa = 6.57TTNNLPIKK583 pKa = 10.54SRR585 pKa = 11.84IKK587 pKa = 10.76NVLNLALYY595 pKa = 10.38DD596 pKa = 3.95SFKK599 pKa = 10.5MAVLTIILILFSYY612 pKa = 9.44TILSILIKK620 pKa = 10.38LRR622 pKa = 11.84RR623 pKa = 11.84LNKK626 pKa = 9.91RR627 pKa = 11.84STHH630 pKa = 5.6KK631 pKa = 11.11VLDD634 pKa = 3.65NTLYY638 pKa = 10.97AVDD641 pKa = 4.04MSHH644 pKa = 7.1LRR646 pKa = 11.84NLGDD650 pKa = 3.28

Molecular weight: 75.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4693

186

2295

586.6

68.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.409 ± 0.52

1.811 ± 0.388

7.053 ± 0.43

6.265 ± 0.312

4.581 ± 0.231

2.813 ± 0.356

1.875 ± 0.198

9.205 ± 0.544

9.163 ± 0.219

9.44 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.441

8.395 ± 0.373

2.365 ± 0.358

2.152 ± 0.295

3.644 ± 0.278

8.161 ± 0.359

4.944 ± 0.207

5.455 ± 0.654

0.533 ± 0.113

6.052 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski