Pelagibacter phage HTVC022P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NU94|A0A4Y1NU94_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC022P OX=2283016 GN=P022_gp26 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 8.02TGDD11 pKa = 3.14NSTTSYY17 pKa = 10.85SIPFSYY23 pKa = 10.6RR24 pKa = 11.84STADD28 pKa = 3.21LTVTLAGSVTTAFSLNSAGTTLTFNSAPAQDD59 pKa = 3.49AAIEE63 pKa = 4.02IRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84TSQTTKK73 pKa = 10.65LVDD76 pKa = 3.6YY77 pKa = 10.81ASGSVLTEE85 pKa = 3.68NDD87 pKa = 3.94LDD89 pKa = 4.06TDD91 pKa = 3.63SDD93 pKa = 3.8QAFFMSQEE101 pKa = 4.56AIDD104 pKa = 4.32DD105 pKa = 4.22AGDD108 pKa = 3.66VIKK111 pKa = 11.1VSNTDD116 pKa = 4.06FQWDD120 pKa = 3.73AQNKK124 pKa = 9.11RR125 pKa = 11.84LTNVADD131 pKa = 3.69PTAAQHH137 pKa = 6.77AATKK141 pKa = 10.58NYY143 pKa = 10.6LEE145 pKa = 4.28NTWLSATDD153 pKa = 3.51KK154 pKa = 10.82ATLNTVNSNMTAINTVNSNITAIGTANTNSANITTVANNIGSVNTVATDD203 pKa = 3.42ISKK206 pKa = 10.95VIAVANDD213 pKa = 3.2LAEE216 pKa = 4.23AVSEE220 pKa = 4.31IEE222 pKa = 4.66TVADD226 pKa = 4.25DD227 pKa = 4.6LNEE230 pKa = 4.22STSEE234 pKa = 3.73IDD236 pKa = 3.59VVSNNIANVNIVGGISSDD254 pKa = 3.06ITSVAGIASAITAVNNNSTNINAVNTNSSNINTVAGDD291 pKa = 3.62STEE294 pKa = 4.08INAVAGNASNINSVAGITSDD314 pKa = 2.97ITSVAGISTAVTAVNNNSTNINAVNSNSSNINTVAGMQSAINTVNSSATAINAVNANATNINTVAGANTNITNVAGGLTNINTVATNLASVNNFAEE410 pKa = 4.49QYY412 pKa = 9.19RR413 pKa = 11.84ISSSAPTTSLNVGDD427 pKa = 5.41LYY429 pKa = 11.47FDD431 pKa = 3.51TTANEE436 pKa = 3.92LKK438 pKa = 10.5VYY440 pKa = 10.44KK441 pKa = 10.46SSGWAAAGSTVNGTSQRR458 pKa = 11.84YY459 pKa = 6.05TYY461 pKa = 10.25NITSAVSSVTGSDD474 pKa = 3.38ANGNTLAYY482 pKa = 9.86DD483 pKa = 3.68AGYY486 pKa = 11.18ADD488 pKa = 4.22VYY490 pKa = 11.21LNGVRR495 pKa = 11.84LSSSDD500 pKa = 3.02ITITSGTSVVFASALSNGDD519 pKa = 3.25VVDD522 pKa = 4.3IVAYY526 pKa = 8.81GTFNVASINAANIDD540 pKa = 3.67SGTINDD546 pKa = 3.73ARR548 pKa = 11.84LPTTMAGKK556 pKa = 8.26TLTTATVEE564 pKa = 4.34ANSLTARR571 pKa = 11.84GDD573 pKa = 3.45GSSADD578 pKa = 3.61GKK580 pKa = 9.41ITLNCSQNSHH590 pKa = 5.66GVSISSPNHH599 pKa = 6.46ASGQSYY605 pKa = 10.79NLILPTSVGTSGQVLATAGSNTNQLSWVDD634 pKa = 3.47ATEE637 pKa = 4.27TKK639 pKa = 9.17PTVADD644 pKa = 3.78VSQTIAPATATTINITGTNFVAIPIVEE671 pKa = 5.06FIKK674 pKa = 10.15TDD676 pKa = 3.55GSITLANTVSFTNATTLSVNVTLATGNYY704 pKa = 8.01HH705 pKa = 6.22VRR707 pKa = 11.84VEE709 pKa = 4.34NPDD712 pKa = 3.32GNAGRR717 pKa = 11.84STNNILTASTAPTFSTSAGSLGTIAGDD744 pKa = 3.67FSGTVATIAGSSDD757 pKa = 3.34SAVTFSEE764 pKa = 4.58TTSVLSTANCTLSSAGVITTTDD786 pKa = 3.7FGGSSTTATTYY797 pKa = 11.26NFIIRR802 pKa = 11.84ITDD805 pKa = 3.47AEE807 pKa = 4.45GQTADD812 pKa = 3.83RR813 pKa = 11.84SFSLTSSFGATGGGQFNN830 pKa = 4.04

Molecular weight: 84.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NUD1|A0A4Y1NUD1_9CAUD Lysozyme murein hydrolase OS=Pelagibacter phage HTVC022P OX=2283016 GN=P022_gp54 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.32FKK6 pKa = 11.11DD7 pKa = 3.37FVVRR11 pKa = 11.84EE12 pKa = 4.09KK13 pKa = 10.49PKK15 pKa = 10.68KK16 pKa = 9.69RR17 pKa = 11.84VRR19 pKa = 11.84THH21 pKa = 7.01KK22 pKa = 10.52KK23 pKa = 9.88RR24 pKa = 11.84LNKK27 pKa = 9.92NEE29 pKa = 3.3KK30 pKa = 9.69RR31 pKa = 11.84DD32 pKa = 3.66YY33 pKa = 11.04KK34 pKa = 10.71KK35 pKa = 10.79YY36 pKa = 10.14NRR38 pKa = 11.84QGRR41 pKa = 11.84PQQ43 pKa = 3.09

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13037

40

1261

241.4

26.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.77 ± 0.397

0.79 ± 0.122

6.037 ± 0.231

6.236 ± 0.447

3.881 ± 0.21

6.689 ± 0.358

1.711 ± 0.163

6.359 ± 0.2

8.23 ± 0.607

7.74 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.272

6.152 ± 0.436

2.938 ± 0.179

3.682 ± 0.273

3.981 ± 0.296

7.348 ± 0.53

7.371 ± 0.653

5.738 ± 0.294

1.158 ± 0.142

3.475 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski