Mobuck virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5NDA7|U5NDA7_9REOV VP3 OS=Mobuck virus OX=1408137 GN=VP3 PE=3 SV=1
MM1 pKa = 7.5EE2 pKa = 6.2GIFSRR7 pKa = 11.84AFCFLEE13 pKa = 3.8CLGANRR19 pKa = 11.84DD20 pKa = 3.26PRR22 pKa = 11.84ARR24 pKa = 11.84RR25 pKa = 11.84TYY27 pKa = 10.52APSDD31 pKa = 3.57GFSLFVTRR39 pKa = 11.84FNATTEE45 pKa = 4.07RR46 pKa = 11.84PITQTPSTVEE56 pKa = 3.5EE57 pKa = 4.31HH58 pKa = 6.49RR59 pKa = 11.84ACFYY63 pKa = 11.34AGLDD67 pKa = 3.67LVIAALGINFNYY79 pKa = 10.32RR80 pKa = 11.84LPNYY84 pKa = 7.84TPNVQVLSILARR96 pKa = 11.84EE97 pKa = 4.13DD98 pKa = 3.51LPYY101 pKa = 8.79TTEE104 pKa = 3.69AFTRR108 pKa = 11.84SSRR111 pKa = 11.84ILNEE115 pKa = 3.73TTAGQSTRR123 pKa = 11.84RR124 pKa = 11.84FTQPWPDD131 pKa = 3.13VSFMYY136 pKa = 10.79LAGTPFRR143 pKa = 11.84TDD145 pKa = 3.36DD146 pKa = 4.51NEE148 pKa = 4.18PQPMAVGYY156 pKa = 11.08NDD158 pKa = 3.65MEE160 pKa = 4.2VTLNVNEE167 pKa = 5.32DD168 pKa = 3.33IEE170 pKa = 4.33ITDD173 pKa = 3.13IVMPNYY179 pKa = 9.51PDD181 pKa = 3.56VVNACITWYY190 pKa = 9.38TLSHH194 pKa = 5.65YY195 pKa = 10.31RR196 pKa = 11.84GAAGALIEE204 pKa = 4.15GSRR207 pKa = 11.84LCTLMIGDD215 pKa = 4.55EE216 pKa = 4.14EE217 pKa = 4.36VDD219 pKa = 3.39AGVEE223 pKa = 4.26LIVPSGTAIRR233 pKa = 11.84VANNSGVNTGIIHH246 pKa = 6.51ITLKK250 pKa = 9.84WFTRR254 pKa = 11.84AQPGEE259 pKa = 4.1HH260 pKa = 6.91LYY262 pKa = 9.68DD263 pKa = 3.62TMLMDD268 pKa = 5.45IDD270 pKa = 3.89ACYY273 pKa = 10.66SFHH276 pKa = 8.85SEE278 pKa = 3.14DD279 pKa = 3.51WYY281 pKa = 10.47EE282 pKa = 3.89LRR284 pKa = 11.84SFLLRR289 pKa = 11.84SVGLPTHH296 pKa = 7.15APPLEE301 pKa = 4.77PIRR304 pKa = 11.84VPNRR308 pKa = 11.84VLALALIARR317 pKa = 11.84LQNVYY322 pKa = 9.7VAHH325 pKa = 6.38SPIDD329 pKa = 3.11IRR331 pKa = 11.84AIQDD335 pKa = 3.17VNGQGGLPNAALEE348 pKa = 4.07ALRR351 pKa = 11.84RR352 pKa = 11.84VV353 pKa = 3.65

Molecular weight: 39.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5NGR3|U5NGR3_9REOV Non-structural protein NS3 OS=Mobuck virus OX=1408137 GN=Nsp3 PE=3 SV=1
MM1 pKa = 7.33HH2 pKa = 7.65AAIQPVKK9 pKa = 10.36RR10 pKa = 11.84EE11 pKa = 3.97NPIPLLTVVPASAPAYY27 pKa = 8.11EE28 pKa = 4.42KK29 pKa = 10.98VFHH32 pKa = 7.08EE33 pKa = 4.25EE34 pKa = 3.96TKK36 pKa = 10.83QKK38 pKa = 8.79EE39 pKa = 4.3TLLNLTVPDD48 pKa = 4.13ALTARR53 pKa = 11.84TTALDD58 pKa = 3.63VLSNALGAGSGTDD71 pKa = 3.0EE72 pKa = 3.66ITRR75 pKa = 11.84RR76 pKa = 11.84EE77 pKa = 3.76RR78 pKa = 11.84SAYY81 pKa = 9.36GAAAQALNEE90 pKa = 4.4DD91 pKa = 3.88EE92 pKa = 4.31STRR95 pKa = 11.84RR96 pKa = 11.84LKK98 pKa = 11.04VYY100 pKa = 9.07MNRR103 pKa = 11.84QIIPRR108 pKa = 11.84LEE110 pKa = 3.82QKK112 pKa = 10.64YY113 pKa = 7.88RR114 pKa = 11.84TSTIKK119 pKa = 10.34YY120 pKa = 8.26RR121 pKa = 11.84LWVCVEE127 pKa = 3.61IFFAFLSIMIFGVMGVTEE145 pKa = 4.18WHH147 pKa = 6.53EE148 pKa = 5.44SIDD151 pKa = 3.79DD152 pKa = 3.65WMKK155 pKa = 9.68KK156 pKa = 7.87QSGIKK161 pKa = 10.17VGVSTVSMICTMILLYY177 pKa = 10.58ASRR180 pKa = 11.84SAGGLKK186 pKa = 9.77EE187 pKa = 3.64LRR189 pKa = 11.84KK190 pKa = 9.42RR191 pKa = 11.84LKK193 pKa = 10.32RR194 pKa = 11.84EE195 pKa = 3.74LIKK198 pKa = 10.8RR199 pKa = 11.84QTYY202 pKa = 10.38NDD204 pKa = 2.99ISGTMGGTEE213 pKa = 3.99THH215 pKa = 6.34GGEE218 pKa = 4.08SDD220 pKa = 3.36SRR222 pKa = 11.84LSNRR226 pKa = 11.84EE227 pKa = 3.01NSAWINAPGVSSPASGWQLMRR248 pKa = 11.84VDD250 pKa = 4.03GG251 pKa = 4.37

Molecular weight: 27.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6255

251

1328

625.5

71.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.347 ± 0.564

1.503 ± 0.184

5.675 ± 0.43

6.938 ± 0.48

3.949 ± 0.365

5.851 ± 0.237

2.126 ± 0.187

6.843 ± 0.275

6.027 ± 0.743

9.081 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.469 ± 0.132

4.317 ± 0.314

3.837 ± 0.446

3.357 ± 0.286

6.763 ± 0.241

6.491 ± 0.388

6.347 ± 0.357

6.507 ± 0.273

1.055 ± 0.19

3.517 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski