Bacillus sp. 7884-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A268JQM5|A0A268JQM5_9BACI RNA polymerase sigma factor OS=Bacillus sp. 7884-1 OX=2021693 GN=CHI06_22250 PE=3 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.92WFDD7 pKa = 4.01RR8 pKa = 11.84VCGEE12 pKa = 4.26LQDD15 pKa = 4.14HH16 pKa = 6.79LEE18 pKa = 4.62SICEE22 pKa = 4.08EE23 pKa = 4.21YY24 pKa = 11.03DD25 pKa = 3.17QVGQMSVEE33 pKa = 3.99RR34 pKa = 11.84AAKK37 pKa = 9.53HH38 pKa = 4.75PRR40 pKa = 11.84IEE42 pKa = 4.29FFVEE46 pKa = 4.09TEE48 pKa = 4.04DD49 pKa = 4.41VNTEE53 pKa = 4.1EE54 pKa = 4.44IDD56 pKa = 3.35RR57 pKa = 11.84DD58 pKa = 4.06YY59 pKa = 11.47FCTLFFDD66 pKa = 4.26PQNEE70 pKa = 3.98EE71 pKa = 4.38FYY73 pKa = 10.64IDD75 pKa = 3.79TFDD78 pKa = 3.5VDD80 pKa = 3.7SGHH83 pKa = 5.15TAKK86 pKa = 10.89VILSDD91 pKa = 3.4IEE93 pKa = 5.13DD94 pKa = 4.29IIDD97 pKa = 3.58EE98 pKa = 4.42VHH100 pKa = 7.14ASLHH104 pKa = 7.26DD105 pKa = 3.73YY106 pKa = 10.92MNDD109 pKa = 3.29DD110 pKa = 4.43DD111 pKa = 6.19HH112 pKa = 6.46YY113 pKa = 9.45TDD115 pKa = 4.69DD116 pKa = 4.47GVYY119 pKa = 8.81LTSDD123 pKa = 2.87EE124 pKa = 4.77MYY126 pKa = 11.07VDD128 pKa = 6.09DD129 pKa = 4.67EE130 pKa = 4.87DD131 pKa = 5.24CYY133 pKa = 11.81EE134 pKa = 4.05MADD137 pKa = 3.8DD138 pKa = 6.2DD139 pKa = 4.18GVEE142 pKa = 4.26YY143 pKa = 11.39YY144 pKa = 10.26EE145 pKa = 4.71AEE147 pKa = 4.22QVNADD152 pKa = 4.69DD153 pKa = 4.18IFEE156 pKa = 4.86EE157 pKa = 5.76IDD159 pKa = 3.78VEE161 pKa = 4.48WNTPEE166 pKa = 3.69VTAFKK171 pKa = 10.83HH172 pKa = 5.1EE173 pKa = 4.6DD174 pKa = 3.76EE175 pKa = 5.29VEE177 pKa = 3.89VTYY180 pKa = 10.95QFGVVPEE187 pKa = 4.39TGDD190 pKa = 3.5GVLKK194 pKa = 10.46RR195 pKa = 11.84INRR198 pKa = 11.84IWTTEE203 pKa = 3.79DD204 pKa = 3.34EE205 pKa = 4.69LIKK208 pKa = 11.03DD209 pKa = 3.48EE210 pKa = 4.42SHH212 pKa = 7.38FIFSKK217 pKa = 10.93EE218 pKa = 3.83EE219 pKa = 3.63ASTIIAMIASHH230 pKa = 6.76MDD232 pKa = 3.12QLSEE236 pKa = 4.74FNFDD240 pKa = 5.1DD241 pKa = 3.93MPP243 pKa = 6.42

Molecular weight: 28.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A268KAU3|A0A268KAU3_9BACI HU family DNA-binding protein OS=Bacillus sp. 7884-1 OX=2021693 GN=CHI06_04770 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5596

0

5596

1653665

27

2744

295.5

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.765 ± 0.036

0.741 ± 0.011

4.933 ± 0.028

7.338 ± 0.041

4.731 ± 0.028

6.991 ± 0.035

2.047 ± 0.017

8.036 ± 0.036

7.043 ± 0.028

9.758 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.016

4.724 ± 0.023

3.698 ± 0.019

3.563 ± 0.019

3.805 ± 0.024

6.102 ± 0.022

5.428 ± 0.032

6.921 ± 0.029

1.096 ± 0.014

3.577 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski