Streptomyces phage Rowa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rowavirus; Streptomyces virus Rowa

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BLV8|A0A2H5BLV8_9CAUD Uncharacterized protein OS=Streptomyces phage Rowa OX=2059883 GN=SEA_ROWA_28 PE=4 SV=1
MM1 pKa = 7.82PKK3 pKa = 9.97LDD5 pKa = 4.34EE6 pKa = 4.91ADD8 pKa = 3.79PYY10 pKa = 10.75NWARR14 pKa = 11.84ALAGADD20 pKa = 3.71DD21 pKa = 4.0EE22 pKa = 4.89TVRR25 pKa = 11.84LPLWTFPPEE34 pKa = 3.66AVPYY38 pKa = 9.84VMHH41 pKa = 6.62EE42 pKa = 3.86RR43 pKa = 11.84RR44 pKa = 11.84VRR46 pKa = 11.84FQWDD50 pKa = 3.62DD51 pKa = 3.61EE52 pKa = 4.87PIGDD56 pKa = 4.28PEE58 pKa = 4.3YY59 pKa = 11.4NN60 pKa = 3.18

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BM35|A0A2H5BM35_9CAUD HNH endonuclease OS=Streptomyces phage Rowa OX=2059883 GN=SEA_ROWA_68 PE=4 SV=1
MM1 pKa = 7.94PSRR4 pKa = 11.84PRR6 pKa = 11.84KK7 pKa = 8.28PCTIPGCPEE16 pKa = 4.01LTPGGGRR23 pKa = 11.84CAEE26 pKa = 4.76HH27 pKa = 7.47KK28 pKa = 10.7AQDD31 pKa = 3.05WQLRR35 pKa = 11.84KK36 pKa = 10.25DD37 pKa = 3.38RR38 pKa = 11.84GAFNYY43 pKa = 10.08GSSKK47 pKa = 6.72WQRR50 pKa = 11.84VRR52 pKa = 11.84RR53 pKa = 11.84AYY55 pKa = 10.7LYY57 pKa = 10.54ANPWCVLCSKK67 pKa = 9.83PATVADD73 pKa = 3.56HH74 pKa = 6.87HH75 pKa = 6.32PLSRR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.98LIARR85 pKa = 11.84GVDD88 pKa = 3.37PDD90 pKa = 4.15TPTHH94 pKa = 6.45LRR96 pKa = 11.84PLCTPCHH103 pKa = 5.39NRR105 pKa = 11.84EE106 pKa = 4.17TARR109 pKa = 11.84HH110 pKa = 5.11QPGGWHH116 pKa = 6.76ANN118 pKa = 3.46

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12804

39

1033

206.5

22.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.684 ± 0.433

1.086 ± 0.16

6.334 ± 0.224

6.248 ± 0.428

2.944 ± 0.201

8.841 ± 0.418

1.64 ± 0.158

3.71 ± 0.202

4.749 ± 0.386

7.88 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.151

3.046 ± 0.187

5.319 ± 0.272

2.788 ± 0.144

6.967 ± 0.401

5.108 ± 0.223

6.803 ± 0.359

7.818 ± 0.326

2.023 ± 0.152

2.819 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski