Bradyrhizobium elkanii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Bradyrhizobium

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1NIU4|A0A0D1NIU4_BRAEL NUDIX hydrolase OS=Bradyrhizobium elkanii OX=29448 GN=QU41_27470 PE=4 SV=1
MM1 pKa = 6.98TVSSATSSTAGTTSSSSSSPSSADD25 pKa = 3.14TVTTTGTTNSTSIDD39 pKa = 3.2WTQLIQAEE47 pKa = 4.68VNAKK51 pKa = 9.63LASATAITTKK61 pKa = 9.21ITANQAKK68 pKa = 9.79ISAYY72 pKa = 9.68TNLQTEE78 pKa = 4.99LSTLASGLTSLSTSIVNSLATNAFATRR105 pKa = 11.84AATISSTGDD114 pKa = 3.17VSASSALNMSVNSGSATGNHH134 pKa = 5.97TLQITQLATAQKK146 pKa = 10.7VIGSSQSSTSTALGLSGTFSLGLAGGTSSAITITSGMSMQDD187 pKa = 2.6IVDD190 pKa = 4.71TINAQTSTTNVQASIVQVSSGSYY213 pKa = 10.38EE214 pKa = 3.93MVLTGTQDD222 pKa = 3.17AANITYY228 pKa = 10.46SSTSGDD234 pKa = 3.96DD235 pKa = 3.36ILNKK239 pKa = 10.49LGVTGTSGAFTNVLQTAQSAEE260 pKa = 3.96FTLDD264 pKa = 4.04GISLTRR270 pKa = 11.84NTNDD274 pKa = 2.85ISDD277 pKa = 4.0VLSGVTFDD285 pKa = 5.33LLQPTPSGTSLNISIQTDD303 pKa = 3.58TSQITSALQSFVTNYY318 pKa = 10.38NAFRR322 pKa = 11.84DD323 pKa = 3.83QVIAQSAQNSDD334 pKa = 3.28GTASSSAVLFGDD346 pKa = 3.27STMRR350 pKa = 11.84DD351 pKa = 2.56IMTQLQQVLSGSVGSMTMADD371 pKa = 3.87LGLSFNQNNEE381 pKa = 3.99LQLDD385 pKa = 3.94TGTLSTVLTQNLAGVTSLLSAQTTTSSSQLSVVNTGTSPQSFTLDD430 pKa = 3.46LTVDD434 pKa = 3.34SSGTLTGASVGGDD447 pKa = 2.94SSGFSVVGNTIIGNAGTIYY466 pKa = 10.87AGMAFNYY473 pKa = 8.74TGSTSQSINVTSTSGLASQIYY494 pKa = 10.09QIAQTNSANSGSLQTLISNLQSTDD518 pKa = 4.37DD519 pKa = 4.59DD520 pKa = 3.66MQTQVNSIQSDD531 pKa = 3.21AATYY535 pKa = 7.53QTQLQTQYY543 pKa = 11.68ANYY546 pKa = 9.29QAAIEE551 pKa = 4.29TANNTLGYY559 pKa = 10.53LKK561 pKa = 10.72ALLNSASSNN570 pKa = 3.46

Molecular weight: 57.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1P2G1|A0A0D1P2G1_BRAEL Taurine catabolism dioxygenase TauD OS=Bradyrhizobium elkanii OX=29448 GN=QU41_13320 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6820

0

6820

2117123

26

4043

310.4

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.604 ± 0.037

0.851 ± 0.011

5.595 ± 0.021

5.085 ± 0.029

3.795 ± 0.019

8.45 ± 0.032

2.001 ± 0.014

5.361 ± 0.021

3.567 ± 0.028

9.91 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.011

2.782 ± 0.018

5.236 ± 0.024

3.206 ± 0.015

6.921 ± 0.033

5.551 ± 0.022

5.449 ± 0.027

7.526 ± 0.022

1.351 ± 0.012

2.307 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski