Rotavirus A (isolate RVA/Dog/United States/Cu-1/1982/G3P5A[3]) (RV-A)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus G3

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6SAK2|B6SAK2_ROTCU Non-structural protein 1 OS=Rotavirus A (isolate RVA/Dog/United States/Cu-1/1982/G3P5A[3]) OX=101357 GN=NSP1 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.2GIEE5 pKa = 4.22YY6 pKa = 7.59TTILTFLISFIFLNYY21 pKa = 8.69MLKK24 pKa = 10.41SLTRR28 pKa = 11.84MMDD31 pKa = 3.5FIIYY35 pKa = 9.69RR36 pKa = 11.84FLFIIVILSPLLKK49 pKa = 10.1AQNYY53 pKa = 8.36GINLPITGSMDD64 pKa = 3.06TAYY67 pKa = 10.65ANSTQEE73 pKa = 4.12EE74 pKa = 4.88TFLTSTLCLYY84 pKa = 10.75YY85 pKa = 8.86PTEE88 pKa = 4.27AATEE92 pKa = 4.11INDD95 pKa = 3.85NSWKK99 pKa = 9.38DD100 pKa = 3.5TLSQLFLTKK109 pKa = 10.05GWPTGSVYY117 pKa = 10.44FKK119 pKa = 10.0EE120 pKa = 4.33YY121 pKa = 10.32TDD123 pKa = 3.31IASFSVDD130 pKa = 3.05PQLYY134 pKa = 9.02CDD136 pKa = 3.89YY137 pKa = 10.88NIVLMKK143 pKa = 10.34YY144 pKa = 10.13DD145 pKa = 3.97AALQLDD151 pKa = 4.06MSEE154 pKa = 4.35LADD157 pKa = 5.13LILNEE162 pKa = 4.24WLCNPMDD169 pKa = 3.29ITLYY173 pKa = 10.67YY174 pKa = 9.25YY175 pKa = 10.55QQTDD179 pKa = 3.57EE180 pKa = 4.17ANKK183 pKa = 8.08WISMGSSCTIKK194 pKa = 10.46VCPLNTQTLGIGCLTTDD211 pKa = 2.69VSTFEE216 pKa = 4.34EE217 pKa = 4.77VATTEE222 pKa = 4.2KK223 pKa = 10.98LVITDD228 pKa = 3.3VVDD231 pKa = 4.31GVNHH235 pKa = 6.68KK236 pKa = 10.86LDD238 pKa = 3.72VTTTTCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 4.01NVAVIQVGGSDD267 pKa = 3.65ILDD270 pKa = 3.33ITADD274 pKa = 3.74PTTAPQTEE282 pKa = 3.66RR283 pKa = 11.84MMRR286 pKa = 11.84INWKK290 pKa = 9.54KK291 pKa = 7.26WWQVFYY297 pKa = 10.66TVVDD301 pKa = 3.52YY302 pKa = 11.53VNQIIQAMSKK312 pKa = 10.21RR313 pKa = 11.84SRR315 pKa = 11.84SLNSAAFYY323 pKa = 11.0YY324 pKa = 10.27RR325 pKa = 11.84VV326 pKa = 3.19

Molecular weight: 37.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6SAK4|B6SAK4_ROTCU Non-structural protein 3 OS=Rotavirus A (isolate RVA/Dog/United States/Cu-1/1982/G3P5A[3]) OX=101357 GN=NSP3 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 11.97KK18 pKa = 10.64FIPFNSLAVKK28 pKa = 10.54CMLTAKK34 pKa = 9.9VDD36 pKa = 3.91KK37 pKa = 10.63KK38 pKa = 11.12DD39 pKa = 3.04QDD41 pKa = 3.14KK42 pKa = 10.51FYY44 pKa = 11.42NSIIYY49 pKa = 9.99GIAPPPQFRR58 pKa = 11.84KK59 pKa = 10.13RR60 pKa = 11.84YY61 pKa = 5.95NTSDD65 pKa = 2.95NSRR68 pKa = 11.84GMNYY72 pKa = 6.85EE73 pKa = 3.33TSMFNKK79 pKa = 9.41VALLICEE86 pKa = 4.4ALNSIKK92 pKa = 9.47VTQSDD97 pKa = 3.6IANVLARR104 pKa = 11.84VVSVRR109 pKa = 11.84HH110 pKa = 5.85LEE112 pKa = 3.93NLVLRR117 pKa = 11.84RR118 pKa = 11.84EE119 pKa = 4.01NHH121 pKa = 5.46QDD123 pKa = 3.23VLFHH127 pKa = 7.13SKK129 pKa = 10.35EE130 pKa = 3.99LLLKK134 pKa = 10.55SVLIAIGHH142 pKa = 5.16SKK144 pKa = 10.37EE145 pKa = 3.97IEE147 pKa = 4.08TTATAEE153 pKa = 3.87GGEE156 pKa = 4.08IVFQNAAFTMWKK168 pKa = 8.48LTYY171 pKa = 10.07LEE173 pKa = 4.72HH174 pKa = 8.22KK175 pKa = 10.27LMPILDD181 pKa = 3.78QNFIEE186 pKa = 4.65YY187 pKa = 10.13KK188 pKa = 9.01ITVNEE193 pKa = 4.45DD194 pKa = 3.01KK195 pKa = 10.56PISEE199 pKa = 4.3SHH201 pKa = 5.4VKK203 pKa = 10.15EE204 pKa = 4.69LIAEE208 pKa = 4.23LRR210 pKa = 11.84WQYY213 pKa = 11.8NKK215 pKa = 10.29FAIITHH221 pKa = 6.19GKK223 pKa = 7.29GHH225 pKa = 5.74YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.16ADD239 pKa = 3.18RR240 pKa = 11.84VYY242 pKa = 10.96ATFKK246 pKa = 10.88SNSKK250 pKa = 10.42NGVNMEE256 pKa = 4.44FNLLDD261 pKa = 5.04QRR263 pKa = 11.84IIWQNWYY270 pKa = 10.72AFTSSMKK277 pKa = 10.28QGNTLDD283 pKa = 3.59VCKK286 pKa = 10.66KK287 pKa = 10.71LLFQKK292 pKa = 9.76MKK294 pKa = 10.47QEE296 pKa = 4.09KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.2MDD310 pKa = 3.45EE311 pKa = 4.05VSQVGII317 pKa = 4.04

Molecular weight: 36.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5797

175

1088

527.0

60.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.279 ± 0.331

1.121 ± 0.282

5.882 ± 0.312

5.486 ± 0.294

4.416 ± 0.293

3.502 ± 0.351

1.501 ± 0.235

7.763 ± 0.369

6.452 ± 0.68

9.332 ± 0.465

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.302

6.848 ± 0.358

3.278 ± 0.3

4.468 ± 0.474

4.606 ± 0.4

8.177 ± 0.91

6.4 ± 0.546

6.227 ± 0.186

1.139 ± 0.146

5.072 ± 0.507
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski