Bradyrhizobium erythrophlei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6G2X9|A0A1N6G2X9_9BRAD Carbon-monoxide dehydrogenase medium subunit OS=Bradyrhizobium erythrophlei OX=1437360 GN=SAMN05443247_01429 PE=4 SV=1
MM1 pKa = 6.89STIPAFTFGGITRR14 pKa = 11.84PALILDD20 pKa = 3.7PSGNIILDD28 pKa = 3.51PAAQAFLDD36 pKa = 4.13TYY38 pKa = 8.75GTKK41 pKa = 10.45ALYY44 pKa = 10.61AGVPPGTHH52 pKa = 6.69FPPIPDD58 pKa = 4.32LLTTPVDD65 pKa = 3.88TNGTANSVAEE75 pKa = 4.3GAAVNTLVGITASSHH90 pKa = 5.49SLLPFSITYY99 pKa = 10.27SLSADD104 pKa = 3.43SSSGGFKK111 pKa = 9.66IDD113 pKa = 3.28PTTGVVSVANSAKK126 pKa = 9.68IDD128 pKa = 3.82FEE130 pKa = 4.5SSPGHH135 pKa = 6.85AYY137 pKa = 8.76TVTVQATDD145 pKa = 4.6GIFTTSQNFTIGVSDD160 pKa = 3.95VAPSTPVDD168 pKa = 3.49GNAATNTIAEE178 pKa = 4.44GAAVGTAVGVTAHH191 pKa = 6.0STDD194 pKa = 3.34INGGTVTYY202 pKa = 10.6SLTGDD207 pKa = 3.4TSGGGFTVNAATGVVTVADD226 pKa = 3.81STKK229 pKa = 9.92IDD231 pKa = 3.79YY232 pKa = 7.64EE233 pKa = 4.35TAPGHH238 pKa = 6.26AYY240 pKa = 10.39SVTVQASDD248 pKa = 3.46GTLTSATQTFTIAVSDD264 pKa = 4.15VPLPTPADD272 pKa = 3.69TNPAANQVAEE282 pKa = 4.45GAAAGTLVGLTATANDD298 pKa = 4.21PNGPTTTYY306 pKa = 11.34SLIGDD311 pKa = 4.04TSGGGFTIDD320 pKa = 3.51PATGVVTVADD330 pKa = 3.69PAKK333 pKa = 10.28IDD335 pKa = 3.59FEE337 pKa = 4.64SAAGHH342 pKa = 6.6AYY344 pKa = 10.33SVTVKK349 pKa = 10.79ADD351 pKa = 3.7DD352 pKa = 4.45GLASTTQTFSIAVTDD367 pKa = 3.99VAPSAPTDD375 pKa = 3.45SDD377 pKa = 3.62ASANTIAEE385 pKa = 4.28GAANGSTVGITAHH398 pKa = 5.68STDD401 pKa = 3.41VNGPAVTYY409 pKa = 10.67SLVGDD414 pKa = 3.99TSGGGFTINAATGVITVADD433 pKa = 3.82STKK436 pKa = 9.89IDD438 pKa = 3.83YY439 pKa = 7.68EE440 pKa = 4.29TSPGHH445 pKa = 6.9AYY447 pKa = 8.8TVTAQASDD455 pKa = 3.54GTLASSQTFTIGVTDD470 pKa = 4.06VPPSNPVDD478 pKa = 3.84SNAAANAVAEE488 pKa = 4.54GAAAGATVGITASSTDD504 pKa = 3.35VNGPAVTYY512 pKa = 10.68SLTGDD517 pKa = 3.4TSGGGFTINSATGVVTVADD536 pKa = 3.88PTKK539 pKa = 10.0IDD541 pKa = 3.74YY542 pKa = 9.46EE543 pKa = 4.44SSPGHH548 pKa = 6.67AYY550 pKa = 10.64SITVQSSDD558 pKa = 3.08GTLTSSQVFTIGVNDD573 pKa = 3.8VAPSTPVDD581 pKa = 3.52SDD583 pKa = 3.19ATANAVAEE591 pKa = 4.49GAAAGTNVGITAHH604 pKa = 6.25SVDD607 pKa = 3.78VNGPAVTYY615 pKa = 10.67SLVGDD620 pKa = 3.99TSGGGFTINSATGVVTVADD639 pKa = 3.56PTKK642 pKa = 10.37IDD644 pKa = 3.84FEE646 pKa = 4.89SSGGSYY652 pKa = 9.14TVVAQSTDD660 pKa = 3.12GTLTNSQAFVIAVTDD675 pKa = 3.77VAPSTPVDD683 pKa = 3.74SNAAANTIAEE693 pKa = 4.36GAAAGSTVGITASSTDD709 pKa = 3.35VNGPAVTYY717 pKa = 10.67SLVGDD722 pKa = 3.99TSGGGFTINATTGVVTVTDD741 pKa = 3.77PTKK744 pKa = 10.13IDD746 pKa = 3.54YY747 pKa = 10.42EE748 pKa = 4.44SAPGHH753 pKa = 6.79AYY755 pKa = 8.84TVTSQASDD763 pKa = 3.4GTLTSSQAFTIAVTDD778 pKa = 3.6VAPSVPVDD786 pKa = 3.31SDD788 pKa = 3.42AAANAVTEE796 pKa = 4.16GAPAGSTVGVTASSTDD812 pKa = 3.38VNGPPVTYY820 pKa = 10.49SLTGDD825 pKa = 3.4TSGGGFTINATTGVVTVADD844 pKa = 4.16PSKK847 pKa = 10.46IDD849 pKa = 3.47YY850 pKa = 10.22EE851 pKa = 4.48SAPGHH856 pKa = 6.4AYY858 pKa = 10.34SVTVTSSDD866 pKa = 2.82GTMANSQVFTIAVNDD881 pKa = 4.12AAPSTPVDD889 pKa = 3.27SDD891 pKa = 3.44AAANTVVEE899 pKa = 4.72GAAAGTTVGVTASSTDD915 pKa = 3.33VNGPAVTYY923 pKa = 10.68SLTGDD928 pKa = 3.4TSGGGFTINAATGVVTVADD947 pKa = 3.56PTKK950 pKa = 10.4IDD952 pKa = 4.04FEE954 pKa = 4.72STAPGHH960 pKa = 6.27TYY962 pKa = 9.93TVTATASDD970 pKa = 3.79GTLASSQIFTIAVTDD985 pKa = 4.47APPSTPVDD993 pKa = 3.16SDD995 pKa = 3.69GAINQVSQNAPVGTYY1010 pKa = 9.26TGLTASSTDD1019 pKa = 3.33VNGPAVTYY1027 pKa = 10.67SLVGDD1032 pKa = 3.97TSGGGFAIDD1041 pKa = 3.44ASTGKK1046 pKa = 8.5VTVADD1051 pKa = 3.61STKK1054 pKa = 9.96IPFNAGSPSFDD1065 pKa = 3.15VTVQSSDD1072 pKa = 3.39GTDD1075 pKa = 2.98TSQQTFTINVIQNTPPVANDD1095 pKa = 3.78DD1096 pKa = 4.05SLSATEE1102 pKa = 5.61AGGLNNSIPGSNPSGNVILGTGSAGAVQDD1131 pKa = 4.3TDD1133 pKa = 3.73AQDD1136 pKa = 3.5PSTALTVVAVHH1147 pKa = 6.6TGPEE1151 pKa = 4.29GGSTGTGTVGSPIVGLHH1168 pKa = 5.31GTLTLDD1174 pKa = 3.06SHH1176 pKa = 6.64GAYY1179 pKa = 9.26TYY1181 pKa = 9.67TVNQTDD1187 pKa = 3.69PTVQALHH1194 pKa = 6.12TAAEE1198 pKa = 4.5TITDD1202 pKa = 3.56QFNYY1206 pKa = 9.51TIQDD1210 pKa = 3.32TGGLQDD1216 pKa = 3.34TATITFTIHH1225 pKa = 6.7GADD1228 pKa = 5.0DD1229 pKa = 4.41LPQAIADD1236 pKa = 3.96TGTMTEE1242 pKa = 4.78DD1243 pKa = 4.38DD1244 pKa = 4.91APTLFNVLANDD1255 pKa = 4.09TLDD1258 pKa = 4.45PDD1260 pKa = 3.68HH1261 pKa = 6.8TALNTIAVGPGSITVTGPAGEE1282 pKa = 4.5TFANTDD1288 pKa = 2.74ATATVVSNEE1297 pKa = 4.09VQVSLNNAHH1306 pKa = 6.32FQQLAVGEE1314 pKa = 4.55HH1315 pKa = 5.31ATITVPYY1322 pKa = 9.14TLTGDD1327 pKa = 3.43TGEE1330 pKa = 4.22TSTANLVVTVNGVNDD1345 pKa = 3.47VPVAVDD1351 pKa = 3.86DD1352 pKa = 4.35SGSMTEE1358 pKa = 4.51DD1359 pKa = 3.27QSGHH1363 pKa = 6.24AFTVLSNDD1371 pKa = 3.74TLDD1374 pKa = 4.77PDD1376 pKa = 3.82HH1377 pKa = 6.9GAPNNVTAGAVTNLSAPAGEE1397 pKa = 5.37GITTADD1403 pKa = 2.8IGVSVNASNQVVVNLGANFQHH1424 pKa = 6.09MQDD1427 pKa = 3.67GQTATFDD1434 pKa = 3.48VAYY1437 pKa = 8.76TLHH1440 pKa = 7.18GDD1442 pKa = 3.7QPGDD1446 pKa = 3.12TSTAEE1451 pKa = 3.77LHH1453 pKa = 5.15VTVTGVNDD1461 pKa = 3.53APVAGNFTFNGVNSATGNTDD1481 pKa = 5.28LIVNDD1486 pKa = 4.46PATTAITEE1494 pKa = 4.24TTPNKK1499 pKa = 9.61TISASLLSGASDD1511 pKa = 3.43VDD1513 pKa = 3.92GPGPLTVVAGTIATAHH1529 pKa = 6.31GGSVTMQSDD1538 pKa = 3.47GDD1540 pKa = 4.34FIYY1543 pKa = 10.36KK1544 pKa = 10.23PPVGYY1549 pKa = 8.84TGSDD1553 pKa = 3.13SFNYY1557 pKa = 9.32QVSDD1561 pKa = 3.6QNTGASGVGLGTGTVTINVAAPKK1584 pKa = 9.48VWYY1587 pKa = 10.41VNADD1591 pKa = 3.18AATDD1595 pKa = 3.83GDD1597 pKa = 4.39GSSEE1601 pKa = 4.09HH1602 pKa = 6.91PFNNLTHH1609 pKa = 6.67FNGAGGVDD1617 pKa = 3.68GAGDD1621 pKa = 4.07TIVLEE1626 pKa = 4.48TATAHH1631 pKa = 4.53YY1632 pKa = 9.47TGGLTLEE1639 pKa = 4.48NNEE1642 pKa = 3.97QLISQSAGVTINGTTLFAASGANAVVDD1669 pKa = 3.87GGVVLGSGNTIEE1681 pKa = 5.24GVDD1684 pKa = 4.19FGTTSGFAVSGSSVGTLHH1702 pKa = 7.37LNDD1705 pKa = 3.32THH1707 pKa = 6.65TGVINNSSGGGISIGGASNVLNVDD1731 pKa = 4.61FNSITTGGGTNGIALTNSSGTFHH1754 pKa = 7.02AHH1756 pKa = 6.79GGTISNASGADD1767 pKa = 3.29VALNGGTVTFTDD1779 pKa = 4.46DD1780 pKa = 3.69GAISDD1785 pKa = 3.86STGTVVSVANMTGNTQAFTGAIGTTGSTDD1814 pKa = 3.1GAIALSSNTGATINFSGGMALSTGASDD1841 pKa = 4.31AFSATGGGTVNVTGTNHH1858 pKa = 6.39LTTTTGTALNISNTTIGSSNVTFHH1882 pKa = 7.67DD1883 pKa = 3.37ISSNGGSSNGIILVNTGSSGGLHH1906 pKa = 4.62VTGDD1910 pKa = 3.56GSTAASGGTIANKK1923 pKa = 9.4TGADD1927 pKa = 3.6GSSTQGTGVFLNNTHH1942 pKa = 6.63DD1943 pKa = 3.7VEE1945 pKa = 4.24LAYY1948 pKa = 9.74MQLNDD1953 pKa = 4.08FSNFAIRR1960 pKa = 11.84GNNVTGFTLDD1970 pKa = 3.28HH1971 pKa = 6.42SVINGDD1977 pKa = 3.4NGTSNSLSDD1986 pKa = 4.16PLASFANVGEE1996 pKa = 4.08DD1997 pKa = 4.02AIRR2000 pKa = 11.84FTNLLGSGSITNSTISGGYY2019 pKa = 7.68TNTIMVEE2026 pKa = 4.08NNTGILNRR2034 pKa = 11.84LTIDD2038 pKa = 2.88HH2039 pKa = 6.08STIGGRR2045 pKa = 11.84TDD2047 pKa = 3.3NGTGMNDD2054 pKa = 4.35GINFQADD2061 pKa = 3.45TGSTAMNLTVTNSALTTARR2080 pKa = 11.84ANLIAVTNQTGTQTDD2095 pKa = 3.56VKK2097 pKa = 10.59FDD2099 pKa = 4.04NNTLTNNHH2107 pKa = 7.2PLTVTGGTAGDD2118 pKa = 3.79FAGLGGMTFDD2128 pKa = 4.5ISNNTFDD2135 pKa = 3.37MHH2137 pKa = 6.8AANSGTGIKK2146 pKa = 10.67SNVIEE2151 pKa = 4.22VNKK2154 pKa = 10.48GSGSGSSTFDD2164 pKa = 3.06GTISGNTIGVAATAHH2179 pKa = 5.95SGSGIGANDD2188 pKa = 3.33IDD2190 pKa = 5.81INSQDD2195 pKa = 3.26NGIFNILVQNNILTHH2210 pKa = 6.51YY2211 pKa = 7.77DD2212 pKa = 3.03TAGIQISQISGSSTVNATVIGNTTSNGDD2240 pKa = 3.54SNAFAGLYY2248 pKa = 9.28VVAGSGLATDD2258 pKa = 3.86NGVVNLKK2265 pKa = 10.65VGGTGTEE2272 pKa = 3.9RR2273 pKa = 11.84NDD2275 pKa = 3.9FSNGDD2280 pKa = 3.82PANGSDD2286 pKa = 4.17VFLQQSSPSSTSVFNLTGSPATNNATLAAITASIQANNLNPGTTTVGTSGTINLVSTTPTLPLLAAPGGVQASSPTTGEE2365 pKa = 3.59MHH2367 pKa = 6.66LTQSEE2372 pKa = 4.33LDD2374 pKa = 3.9SVVAAAIAQWAAAGASASQLAALHH2398 pKa = 6.26AATFSVADD2406 pKa = 4.11LSGSIIGDD2414 pKa = 3.49EE2415 pKa = 4.3SSPAHH2420 pKa = 5.69ITIDD2424 pKa = 3.17TDD2426 pKa = 3.62AAGHH2430 pKa = 6.07GWFIDD2435 pKa = 3.63PTPSDD2440 pKa = 3.4NSEE2443 pKa = 3.77FTHH2446 pKa = 6.13AQNAAGTDD2454 pKa = 3.83LLTDD2458 pKa = 3.89PTNAAAGHH2466 pKa = 7.0LDD2468 pKa = 3.44LLTTVSHH2475 pKa = 6.2EE2476 pKa = 4.16MGHH2479 pKa = 5.95VLGLPDD2485 pKa = 3.85STSPSAVNDD2494 pKa = 3.38LMYY2497 pKa = 11.04VSLVDD2502 pKa = 3.55GEE2504 pKa = 4.58RR2505 pKa = 11.84RR2506 pKa = 11.84LPDD2509 pKa = 3.77AADD2512 pKa = 3.33VAQANGSTFSFQAVGTTAPVVTAAPVLDD2540 pKa = 4.74AGHH2543 pKa = 6.87GGGTLVGTAGADD2555 pKa = 3.02NFVFANVDD2563 pKa = 3.35VHH2565 pKa = 6.86GSAPPPITHH2574 pKa = 6.18VANYY2578 pKa = 10.42SFAQGDD2584 pKa = 3.8TFDD2587 pKa = 4.95FSVLTSQLHH2596 pKa = 5.53GSGVSDD2602 pKa = 3.65ALVVRR2607 pKa = 11.84AIEE2610 pKa = 4.17GAGGSFATLQVNTADD2625 pKa = 3.43SSMGSKK2631 pKa = 10.3AGSNWVSVAQIDD2643 pKa = 4.14GAHH2646 pKa = 6.76AGDD2649 pKa = 4.15ALNVLVDD2656 pKa = 3.27THH2658 pKa = 6.34SAVHH2662 pKa = 6.68ASQIHH2667 pKa = 5.74VDD2669 pKa = 3.86LLVV2672 pKa = 3.37

Molecular weight: 263.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6ENP2|A0A1N6ENP2_9BRAD Uncharacterized conserved protein contains HEPN domain OS=Bradyrhizobium erythrophlei OX=1437360 GN=SAMN05443247_00159 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.25VRR4 pKa = 11.84NSLKK8 pKa = 10.15SLRR11 pKa = 11.84GRR13 pKa = 11.84HH14 pKa = 5.06RR15 pKa = 11.84NNRR18 pKa = 11.84LVRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.2GRR25 pKa = 11.84VYY27 pKa = 10.74VINKK31 pKa = 4.67VQRR34 pKa = 11.84RR35 pKa = 11.84FKK37 pKa = 10.83ARR39 pKa = 11.84QGG41 pKa = 3.34

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10605

0

10605

2999090

26

3139

282.8

30.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.093 ± 0.032

0.945 ± 0.008

5.415 ± 0.019

5.273 ± 0.022

3.826 ± 0.015

8.155 ± 0.026

2.113 ± 0.012

5.425 ± 0.017

3.751 ± 0.023

9.814 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.012

2.854 ± 0.018

5.32 ± 0.019

3.232 ± 0.015

7.144 ± 0.033

5.773 ± 0.02

5.378 ± 0.025

7.344 ± 0.02

1.403 ± 0.01

2.31 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski