Streptococcus phage phiZJ20091101-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I614|A0A1X9I614_9CAUD Uncharacterized protein OS=Streptococcus phage phiZJ20091101-4 OX=1860193 PE=4 SV=1
MM1 pKa = 7.5TIKK4 pKa = 10.52EE5 pKa = 4.59LIKK8 pKa = 9.91EE9 pKa = 4.02LQMYY13 pKa = 10.62DD14 pKa = 3.3EE15 pKa = 5.17DD16 pKa = 5.01KK17 pKa = 11.27EE18 pKa = 4.59VVLTIANVYY27 pKa = 9.12PVLHH31 pKa = 6.45EE32 pKa = 4.29FVDD35 pKa = 4.26LEE37 pKa = 4.38TGLVRR42 pKa = 11.84LSSDD46 pKa = 3.45CQIGFEE52 pKa = 4.78FNLLSDD58 pKa = 3.7NRR60 pKa = 11.84LEE62 pKa = 5.37IEE64 pKa = 4.58GVWW67 pKa = 3.21

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I737|A0A1X9I737_9CAUD Uncharacterized protein OS=Streptococcus phage phiZJ20091101-4 OX=1860193 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.21KK7 pKa = 9.56KK8 pKa = 10.35YY9 pKa = 9.93RR10 pKa = 11.84LIQALEE16 pKa = 3.77QWLLAISTTIDD27 pKa = 3.39IIVNGQNRR35 pKa = 11.84LYY37 pKa = 11.14NRR39 pKa = 11.84VAEE42 pKa = 4.27LEE44 pKa = 4.16NIIEE48 pKa = 4.31RR49 pKa = 11.84NAQATNSRR57 pKa = 11.84FDD59 pKa = 3.65YY60 pKa = 10.95LEE62 pKa = 4.15KK63 pKa = 10.68KK64 pKa = 9.57VANKK68 pKa = 10.12LSKK71 pKa = 10.66KK72 pKa = 9.69SWFSRR77 pKa = 11.84KK78 pKa = 8.79

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12764

42

1756

199.4

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.513 ± 0.35

0.658 ± 0.121

6.416 ± 0.269

7.396 ± 0.509

4.121 ± 0.288

6.033 ± 0.483

1.567 ± 0.179

7.075 ± 0.208

8.03 ± 0.361

8.516 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.227

5.171 ± 0.32

2.844 ± 0.27

4.238 ± 0.222

4.857 ± 0.343

5.641 ± 0.325

6.189 ± 0.692

6.369 ± 0.249

1.316 ± 0.132

4.019 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski