Pseudomonas phage AF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4NZ84|K4NZ84_9CAUD Uncharacterized protein OS=Pseudomonas phage AF OX=1235689 GN=AF_063 PE=4 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.36PDD5 pKa = 3.22WKK7 pKa = 10.62DD8 pKa = 3.2APEE11 pKa = 4.24GFEE14 pKa = 5.26YY15 pKa = 10.76LAQDD19 pKa = 5.57KK20 pKa = 11.05DD21 pKa = 4.28GEE23 pKa = 4.53WYY25 pKa = 8.35WWISEE30 pKa = 4.21PFADD34 pKa = 4.06TNLGYY39 pKa = 10.43WMSDD43 pKa = 3.14FKK45 pKa = 11.94NNDD48 pKa = 3.0CRR50 pKa = 11.84DD51 pKa = 3.59AGLVEE56 pKa = 4.84PEE58 pKa = 5.11PNPDD62 pKa = 2.89WQEE65 pKa = 3.9TLEE68 pKa = 4.09RR69 pKa = 11.84RR70 pKa = 11.84PP71 pKa = 3.45

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4NZ61|K4NZ61_9CAUD Putative recombination protein NinB OS=Pseudomonas phage AF OX=1235689 GN=AF_037 PE=4 SV=1
MM1 pKa = 7.56IKK3 pKa = 10.02QRR5 pKa = 11.84VYY7 pKa = 11.09SFRR10 pKa = 11.84DD11 pKa = 2.95LMQRR15 pKa = 11.84LNHH18 pKa = 6.85PDD20 pKa = 2.87WAVAAIGKK28 pKa = 9.54DD29 pKa = 3.46FFFVPRR35 pKa = 11.84SYY37 pKa = 11.49LPGRR41 pKa = 11.84KK42 pKa = 8.35II43 pKa = 3.6

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13753

43

2213

211.6

23.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.328 ± 0.409

1.091 ± 0.211

6.064 ± 0.308

6.297 ± 0.397

3.287 ± 0.174

7.504 ± 0.293

1.6 ± 0.157

5.068 ± 0.174

4.908 ± 0.331

7.86 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.207

3.803 ± 0.24

4.515 ± 0.21

5.119 ± 0.423

6.13 ± 0.308

6.006 ± 0.307

5.89 ± 0.537

6.246 ± 0.275

1.672 ± 0.19

2.828 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski