Mycobacterium phage B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5XV90|A0A2Z5XV90_9CAUD Putative esterase/lipase OS=Mycobacterium phage B1 OX=2077133 PE=4 SV=1
MM1 pKa = 7.52MLTQEE6 pKa = 4.41DD7 pKa = 4.94FEE9 pKa = 6.42AEE11 pKa = 4.22LVQDD15 pKa = 4.12SYY17 pKa = 11.9YY18 pKa = 11.25DD19 pKa = 3.48GMNMFEE25 pKa = 4.27QAAQAGLEE33 pKa = 3.96GRR35 pKa = 11.84NYY37 pKa = 10.83LL38 pKa = 4.3

Molecular weight: 4.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5XV84|A0A2Z5XV84_9CAUD Uncharacterized protein OS=Mycobacterium phage B1 OX=2077133 PE=4 SV=1
MM1 pKa = 6.94IVARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VALGVASAGTVAVGGLAFALSFTALRR33 pKa = 11.84DD34 pKa = 3.56LSASNGVAQAWMVPLVVDD52 pKa = 3.79GGIIVATAATVALRR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 5.22QWYY71 pKa = 9.42AWTLLILSSLVSVAGNVAHH90 pKa = 6.57AQAHH94 pKa = 5.49GAIAMVIAAIPPLWLLAATHH114 pKa = 5.73LTVMLSRR121 pKa = 11.84SDD123 pKa = 3.85KK124 pKa = 10.87EE125 pKa = 4.24PVPVAAEE132 pKa = 3.9PLHH135 pKa = 6.32IANAAA140 pKa = 3.29

Molecular weight: 14.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13722

38

1014

188.0

20.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.678 ± 0.406

0.743 ± 0.11

6.391 ± 0.239

6.843 ± 0.376

3.345 ± 0.212

8.177 ± 0.53

2.092 ± 0.202

4.722 ± 0.158

4.307 ± 0.204

8.279 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.463 ± 0.127

3.571 ± 0.227

5.764 ± 0.396

3.848 ± 0.342

6.69 ± 0.325

5.109 ± 0.244

5.728 ± 0.222

7.39 ± 0.236

1.946 ± 0.139

2.915 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski