Microbacterium phage Sansa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Elerivirus; unclassified Elerivirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KZX9|A0A345KZX9_9CAUD Uncharacterized protein OS=Microbacterium phage Sansa OX=2250298 GN=8 PE=4 SV=1
MM1 pKa = 7.75AAIDD5 pKa = 4.69DD6 pKa = 4.49LNALLANLPGFSLISEE22 pKa = 4.36SMKK25 pKa = 10.65LAALEE30 pKa = 4.16GARR33 pKa = 11.84VPDD36 pKa = 4.76SFGIWPGEE44 pKa = 3.7QGYY47 pKa = 10.44EE48 pKa = 3.98STYY51 pKa = 11.22DD52 pKa = 3.3VYY54 pKa = 10.96FAAISLIGFLKK65 pKa = 10.5AQPFITNTNSEE76 pKa = 4.37GTGVTIQAPDD86 pKa = 3.14WSALLIYY93 pKa = 10.3YY94 pKa = 9.97SSQSTIVQAAGNGVLTKK111 pKa = 10.64VNIPDD116 pKa = 3.7TPHH119 pKa = 4.95VQKK122 pKa = 11.12VPMNYY127 pKa = 10.4GGDD130 pKa = 3.4QYY132 pKa = 12.1GDD134 pKa = 4.4LDD136 pKa = 3.77TDD138 pKa = 3.61MGG140 pKa = 4.06

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KZZ0|A0A345KZZ0_9CAUD Uncharacterized protein OS=Microbacterium phage Sansa OX=2250298 GN=19 PE=4 SV=1
MM1 pKa = 7.42AARR4 pKa = 11.84RR5 pKa = 11.84ATFRR9 pKa = 11.84NPSSYY14 pKa = 10.77KK15 pKa = 8.07QTLTIKK21 pKa = 10.72VKK23 pKa = 9.55VTTASGNVKK32 pKa = 8.76VTDD35 pKa = 5.61LILQAGTTGTGWLPNVTEE53 pKa = 4.56MPWTTGVVSS62 pKa = 3.9

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12597

45

808

203.2

22.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.629 ± 0.365

0.643 ± 0.083

6.136 ± 0.281

6.668 ± 0.436

3.032 ± 0.181

8.256 ± 0.436

1.802 ± 0.169

5.096 ± 0.347

5.001 ± 0.387

8.288 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.166

3.342 ± 0.178

4.7 ± 0.229

3.437 ± 0.162

5.962 ± 0.387

6.248 ± 0.316

6.771 ± 0.358

7.391 ± 0.265

1.921 ± 0.226

3.048 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski