Streptomyces phage Wofford

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Karimacvirus; Streptomyces virus Wollford

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345M9S3|A0A345M9S3_9CAUD Portal protein OS=Streptomyces phage Wofford OX=2283267 GN=48 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.54QLQVLADD10 pKa = 3.64APFSYY15 pKa = 9.81WKK17 pKa = 10.48LDD19 pKa = 3.49GNGPVYY25 pKa = 10.33EE26 pKa = 4.92DD27 pKa = 3.18SAGSFRR33 pKa = 11.84QADD36 pKa = 3.94LTATAIPHH44 pKa = 6.43PALVTGSGNALVLQNSNRR62 pKa = 11.84LQMDD66 pKa = 3.38DD67 pKa = 3.45PVFNKK72 pKa = 10.35GYY74 pKa = 8.74EE75 pKa = 3.82ARR77 pKa = 11.84QWSLEE82 pKa = 3.77AWIKK86 pKa = 9.92PLSINGEE93 pKa = 3.91ISIMSHH99 pKa = 5.46SGNYY103 pKa = 9.83DD104 pKa = 3.14GLTITPTTINFRR116 pKa = 11.84TKK118 pKa = 10.81YY119 pKa = 8.41LTAPASEE126 pKa = 4.02INYY129 pKa = 9.55IYY131 pKa = 11.0NSTKK135 pKa = 9.48AFHH138 pKa = 6.13VVGVHH143 pKa = 5.12TNGKK147 pKa = 9.3NSLYY151 pKa = 10.82VDD153 pKa = 3.91GNLVAEE159 pKa = 4.45IDD161 pKa = 3.55ITDD164 pKa = 3.63EE165 pKa = 4.6QEE167 pKa = 3.12IDD169 pKa = 3.27AYY171 pKa = 11.5NFLTSDD177 pKa = 4.61LIAGEE182 pKa = 4.45SVSTSKK188 pKa = 10.64IAIDD192 pKa = 3.79SPAVYY197 pKa = 10.43AFALPADD204 pKa = 4.58TIKK207 pKa = 10.66KK208 pKa = 9.95HH209 pKa = 5.41YY210 pKa = 10.15DD211 pKa = 3.21CGVDD215 pKa = 3.67VEE217 pKa = 4.81SSDD220 pKa = 5.22AIAGFNDD227 pKa = 3.13ASHH230 pKa = 6.9WNFSDD235 pKa = 3.32EE236 pKa = 4.24SRR238 pKa = 11.84NIAVTKK244 pKa = 10.48LWDD247 pKa = 3.96TEE249 pKa = 4.38EE250 pKa = 4.06EE251 pKa = 4.36WPDD254 pKa = 3.53GVIDD258 pKa = 3.94NVVIANDD265 pKa = 4.6FIVPSYY271 pKa = 10.25EE272 pKa = 3.75QTEE275 pKa = 4.41TEE277 pKa = 4.25EE278 pKa = 4.33VVDD281 pKa = 4.12GLIVPIYY288 pKa = 9.53TNTSIPGTWIGSVPLGMTLAGISDD312 pKa = 4.67AIIRR316 pKa = 11.84YY317 pKa = 9.19RR318 pKa = 11.84GEE320 pKa = 3.83GSFTIEE326 pKa = 4.23CSTDD330 pKa = 4.26NGTTWQDD337 pKa = 2.47VDD339 pKa = 3.73NAPSFDD345 pKa = 4.33LDD347 pKa = 3.63EE348 pKa = 4.69TDD350 pKa = 3.86SIGIKK355 pKa = 10.4VSFTGGIVDD364 pKa = 3.84DD365 pKa = 4.15QSYY368 pKa = 10.62VDD370 pKa = 3.69YY371 pKa = 10.62VQVIGYY377 pKa = 7.66EE378 pKa = 4.12DD379 pKa = 3.97KK380 pKa = 11.15NINGTRR386 pKa = 11.84SDD388 pKa = 3.29RR389 pKa = 11.84AAVLNGTGISSIKK402 pKa = 8.94TYY404 pKa = 10.84QPIEE408 pKa = 4.0YY409 pKa = 10.25ADD411 pKa = 4.18DD412 pKa = 3.91NGLLILSGDD421 pKa = 3.17ISVGIDD427 pKa = 3.09SSYY430 pKa = 11.5DD431 pKa = 3.48GEE433 pKa = 4.47EE434 pKa = 4.11EE435 pKa = 5.35PGDD438 pKa = 3.97LDD440 pKa = 4.89INGIDD445 pKa = 3.52MWVKK449 pKa = 9.74PVAGNIISAGANSISRR465 pKa = 11.84VGNTITFSGYY475 pKa = 10.75SDD477 pKa = 3.37VTVNGVSVASGATVFVNDD495 pKa = 2.75SWYY498 pKa = 10.58HH499 pKa = 5.26IGGVFSTPGNYY510 pKa = 9.83PITIGTANARR520 pKa = 11.84IGPASAIYY528 pKa = 10.7GDD530 pKa = 3.73ISLAGLQQIYY540 pKa = 10.65ASYY543 pKa = 10.79LGLPGLTVDD552 pKa = 4.52DD553 pKa = 4.73EE554 pKa = 4.84SVIGITTPADD564 pKa = 3.35EE565 pKa = 4.84ALLYY569 pKa = 10.1AHH571 pKa = 6.72VWGITPAGG579 pKa = 3.48

Molecular weight: 62.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MA51|A0A345MA51_9CAUD Uncharacterized protein OS=Streptomyces phage Wofford OX=2283267 GN=236 PE=4 SV=1
MM1 pKa = 7.57SVVLDD6 pKa = 4.06YY7 pKa = 11.73NSVEE11 pKa = 4.11AFVTEE16 pKa = 4.08QRR18 pKa = 11.84KK19 pKa = 9.36LKK21 pKa = 10.82NDD23 pKa = 3.45VRR25 pKa = 11.84WDD27 pKa = 2.86GWTLVFFRR35 pKa = 11.84KK36 pKa = 9.47SRR38 pKa = 11.84NFSGWSKK45 pKa = 10.99PNGAYY50 pKa = 10.41RR51 pKa = 11.84NNNWGFEE58 pKa = 3.91TRR60 pKa = 11.84IAVGNDD66 pKa = 3.3GKK68 pKa = 10.3WRR70 pKa = 11.84VPSRR74 pKa = 11.84NVRR77 pKa = 11.84NSS79 pKa = 2.7

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

33912

30

2084

160.0

17.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.212 ± 0.358

1.094 ± 0.131

6.585 ± 0.179

6.927 ± 0.321

3.786 ± 0.134

7.531 ± 0.26

1.743 ± 0.128

5.659 ± 0.165

6.487 ± 0.295

7.098 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.13

4.751 ± 0.141

3.651 ± 0.15

3.279 ± 0.179

5.547 ± 0.188

6.045 ± 0.253

5.939 ± 0.312

7.042 ± 0.203

1.881 ± 0.106

3.766 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski