Humibacter sp. WJ7-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Humibacter; unclassified Humibacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3604 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8M5N6|A0A5B8M5N6_9MICO DUF1211 domain-containing protein OS=Humibacter sp. WJ7-1 OX=2599293 GN=FPZ11_12085 PE=3 SV=1
MM1 pKa = 7.07SHH3 pKa = 6.62HH4 pKa = 6.91RR5 pKa = 11.84LITLSALALTAVATTFLLGTAPASASPVTASSTFPATIMTGDD47 pKa = 3.72WIDD50 pKa = 3.63GQLEE54 pKa = 3.91FDD56 pKa = 4.26VPADD60 pKa = 3.62ATPDD64 pKa = 3.58EE65 pKa = 5.21LPTSVSFSVASAADD79 pKa = 3.78PKK81 pKa = 11.14ASAVQFSLEE90 pKa = 4.43DD91 pKa = 3.48MSSDD95 pKa = 3.19LTNCVVDD102 pKa = 3.75PSGASGSCDD111 pKa = 2.75WTDD114 pKa = 3.17AAPGQSEE121 pKa = 4.71TIGLEE126 pKa = 3.82EE127 pKa = 4.0TAVDD131 pKa = 3.59SSIGVQHH138 pKa = 6.55LTLSEE143 pKa = 4.01QTPGGTVAVGAQDD156 pKa = 3.23INVIDD161 pKa = 4.12SVVSGSATVSSHH173 pKa = 4.34TVAIGDD179 pKa = 4.2TVTVTATFTIDD190 pKa = 3.15PGTPDD195 pKa = 4.46DD196 pKa = 4.2YY197 pKa = 11.63LPSFVQFMTATDD209 pKa = 3.77EE210 pKa = 4.43ASVASSLTYY219 pKa = 10.76KK220 pKa = 10.73LLSFSGASSCQVSPDD235 pKa = 3.45TKK237 pKa = 11.08GIGCQLDD244 pKa = 3.71DD245 pKa = 4.81PQPGDD250 pKa = 3.72TITVTGEE257 pKa = 3.51ATATLTQLGDD267 pKa = 3.56SGPNPLGVHH276 pKa = 5.93HH277 pKa = 7.26VYY279 pKa = 10.59LGYY282 pKa = 10.72GGPGLPAIPQEE293 pKa = 4.94QSDD296 pKa = 3.56QDD298 pKa = 3.67ITVVPAASPGAGSGTGGSSASPSSGAVTAGPSSATDD334 pKa = 3.51PASGEE339 pKa = 4.03LAATGTDD346 pKa = 2.44AWTSVAGGGILLLCGAVVLALRR368 pKa = 11.84SRR370 pKa = 11.84RR371 pKa = 11.84SRR373 pKa = 11.84AA374 pKa = 3.09

Molecular weight: 37.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8M8Z8|A0A5B8M8Z8_9MICO DNA helicase OS=Humibacter sp. WJ7-1 OX=2599293 GN=FPZ11_05875 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3604

0

3604

1195877

27

6917

331.8

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.306 ± 0.06

0.55 ± 0.01

6.227 ± 0.037

5.135 ± 0.045

3.126 ± 0.028

8.984 ± 0.044

2.096 ± 0.019

4.436 ± 0.032

2.112 ± 0.033

9.876 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.781 ± 0.018

2.12 ± 0.03

5.233 ± 0.029

2.887 ± 0.024

7.064 ± 0.063

6.076 ± 0.045

6.331 ± 0.066

9.034 ± 0.04

1.544 ± 0.021

2.083 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski