Acidocella sp. MX-AZ02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidocella; unclassified Acidocella

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3506 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K5YLL3|K5YLL3_9PROT ImpA domain-containing protein OS=Acidocella sp. MX-AZ02 OX=1214225 GN=MXAZACID_08866 PE=4 SV=1
MM1 pKa = 7.57SGLTNAVNAALSGLQLFEE19 pKa = 5.71AGISTVSNNLANANTAGYY37 pKa = 7.74TVEE40 pKa = 4.36NVNAQTAQAAAGQPGAGVLPAQITRR65 pKa = 11.84AASGFAASQLRR76 pKa = 11.84TANSASAAASAQSTALTDD94 pKa = 3.48ISNALTNNGNVQTAINQFFSDD115 pKa = 3.82IGSLAASPSSSALRR129 pKa = 11.84QTVLSDD135 pKa = 2.95AGNVVGTFQTAATSIASVVSGQQAGLSSNVTAANQILQQLSTLNKK180 pKa = 10.06SLQTAPNSPSLLDD193 pKa = 3.67QRR195 pKa = 11.84DD196 pKa = 3.54SALNALSQYY205 pKa = 10.63LPVNIIPQSDD215 pKa = 3.71GGVVVATGGTVLVDD229 pKa = 3.21QSGVQALALAEE240 pKa = 4.1NANGQTTLTAGNNQMKK256 pKa = 8.92LTLGEE261 pKa = 4.21SDD263 pKa = 3.65GSLGATLANIVAGGQATQSLSALAAVFSAQVNTAQAQGLTPTGTSGTPIFSVPAPSVAPSGGNSGSAVITASLSNEE339 pKa = 3.61AALPANGGPFTLTYY353 pKa = 10.49NSTSGWSAVDD363 pKa = 3.32QANGQSYY370 pKa = 10.03IVGGTPPAFAGLALSISGTANNGDD394 pKa = 3.85SFTVNPAPGAANAIAVAATTPNDD417 pKa = 3.03IAAADD422 pKa = 4.5PYY424 pKa = 11.09VATNGTLQTDD434 pKa = 4.1GSVINKK440 pKa = 9.09NAGTINVGTDD450 pKa = 3.12SVTSSPASGSAVVPASYY467 pKa = 10.61YY468 pKa = 10.21GQNLLLTFTSASAYY482 pKa = 10.01NVSTLASPGTIIASGTLGSNGGNVAVAYY510 pKa = 9.2PSGLASGQYY519 pKa = 9.42WNLPLTGAPVAGDD532 pKa = 3.83TLSLSPGGSASGSNAQRR549 pKa = 11.84LANLWTATGTTSSGTLEE566 pKa = 3.84QSFVGLSTDD575 pKa = 3.81LGANAAAAQALATSTGSQVTAATTNLATIAGVNSDD610 pKa = 3.53QQAVLMTNYY619 pKa = 9.17EE620 pKa = 3.91QAYY623 pKa = 8.66QAAAKK628 pKa = 10.01AISAANTMFDD638 pKa = 3.68SLLNAII644 pKa = 4.92

Molecular weight: 62.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K5ZEQ9|K5ZEQ9_9PROT TonB-dependent receptor OS=Acidocella sp. MX-AZ02 OX=1214225 GN=MXAZACID_17201 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84MEE4 pKa = 4.86ARR6 pKa = 11.84MHH8 pKa = 6.77DD9 pKa = 4.72LVTLGPWLRR18 pKa = 11.84RR19 pKa = 11.84FLSEE23 pKa = 4.97HH24 pKa = 6.12IVTEE28 pKa = 4.18RR29 pKa = 11.84NLARR33 pKa = 11.84NTRR36 pKa = 11.84SSYY39 pKa = 11.14RR40 pKa = 11.84DD41 pKa = 3.5TFSLLLPFISRR52 pKa = 11.84KK53 pKa = 8.65LRR55 pKa = 11.84KK56 pKa = 9.05PVDD59 pKa = 3.31RR60 pKa = 11.84LAVRR64 pKa = 11.84DD65 pKa = 4.24LSTQHH70 pKa = 6.18VLQFLAHH77 pKa = 6.31LEE79 pKa = 3.94EE80 pKa = 5.61DD81 pKa = 3.9RR82 pKa = 11.84GCSARR87 pKa = 11.84TRR89 pKa = 11.84NQRR92 pKa = 11.84LAAIRR97 pKa = 11.84AFARR101 pKa = 11.84FIGSRR106 pKa = 11.84DD107 pKa = 3.33PAHH110 pKa = 7.05IEE112 pKa = 3.65WCGHH116 pKa = 4.01IRR118 pKa = 11.84AIASKK123 pKa = 10.76KK124 pKa = 10.8SMQQPVGWLARR135 pKa = 11.84PEE137 pKa = 4.09MEE139 pKa = 5.49AMLAVPDD146 pKa = 3.82RR147 pKa = 11.84KK148 pKa = 8.52TPRR151 pKa = 11.84GRR153 pKa = 11.84DD154 pKa = 3.33EE155 pKa = 4.0YY156 pKa = 11.8ALLLFLYY163 pKa = 8.54NTGARR168 pKa = 11.84VSEE171 pKa = 3.95ATQLKK176 pKa = 9.99VRR178 pKa = 11.84DD179 pKa = 3.78LQLEE183 pKa = 4.21HH184 pKa = 7.04GNRR187 pKa = 11.84GHH189 pKa = 7.58DD190 pKa = 3.59LVTLHH195 pKa = 6.75GKK197 pKa = 9.45GGKK200 pKa = 7.38TRR202 pKa = 11.84QCPLWPEE209 pKa = 4.43TKK211 pKa = 10.29AVLAQRR217 pKa = 11.84VLGRR221 pKa = 11.84AAEE224 pKa = 4.04NAVFVSRR231 pKa = 11.84LGTPFTRR238 pKa = 11.84FGVYY242 pKa = 10.12RR243 pKa = 11.84LIEE246 pKa = 4.05RR247 pKa = 11.84CAARR251 pKa = 11.84VPALAGRR258 pKa = 11.84TITPHH263 pKa = 6.44VIRR266 pKa = 11.84HH267 pKa = 4.71TTACHH272 pKa = 5.6LVLAGVDD279 pKa = 3.5INTVRR284 pKa = 11.84AWLGHH289 pKa = 4.67VSISTTNIYY298 pKa = 11.05AEE300 pKa = 3.93IDD302 pKa = 3.25ITLKK306 pKa = 10.45AQAVALCEE314 pKa = 4.01VGQPQLGRR322 pKa = 11.84SWKK325 pKa = 9.71NDD327 pKa = 3.16KK328 pKa = 11.22DD329 pKa = 3.59LMAFLKK335 pKa = 10.89SLL337 pKa = 3.63

Molecular weight: 38.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3506

0

3506

1058590

25

1979

301.9

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.943 ± 0.06

0.9 ± 0.013

4.836 ± 0.031

5.244 ± 0.044

3.658 ± 0.026

8.871 ± 0.05

2.111 ± 0.019

5.028 ± 0.031

3.103 ± 0.033

11.161 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.019

2.652 ± 0.033

5.503 ± 0.04

3.678 ± 0.03

6.432 ± 0.046

5.1 ± 0.04

5.058 ± 0.037

6.772 ± 0.033

1.326 ± 0.018

2.199 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski