Pisolithus tinctorius Marx 270

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Sclerodermatineae; Pisolithaceae; Pisolithus; Pisolithus tinctorius

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C3PCU5|A0A0C3PCU5_PISTI Uncharacterized protein OS=Pisolithus tinctorius Marx 270 OX=870435 GN=M404DRAFT_25136 PE=4 SV=1
MM1 pKa = 7.21QLHH4 pKa = 7.13CIGFIPHH11 pKa = 7.27EE12 pKa = 4.78DD13 pKa = 3.2PGTFGFLDD21 pKa = 3.71PHH23 pKa = 7.21HH24 pKa = 7.01VICAIHH30 pKa = 7.06LIPAFKK36 pKa = 10.61KK37 pKa = 10.77EE38 pKa = 3.79NDD40 pKa = 3.64EE41 pKa = 3.9DD42 pKa = 3.74WVYY45 pKa = 11.5LFVDD49 pKa = 4.58RR50 pKa = 11.84DD51 pKa = 3.48MLMCFHH57 pKa = 7.14GGSIGHH63 pKa = 7.15KK64 pKa = 7.42STCTATNVFKK74 pKa = 10.8QDD76 pKa = 3.69CDD78 pKa = 4.03DD79 pKa = 5.16LDD81 pKa = 3.92RR82 pKa = 11.84TCDD85 pKa = 4.31FSTTDD90 pKa = 3.34NEE92 pKa = 3.87EE93 pKa = 4.18HH94 pKa = 6.58EE95 pKa = 5.28NITGMSTIVSPDD107 pKa = 3.67DD108 pKa = 4.28EE109 pKa = 4.57IQAEE113 pKa = 4.08DD114 pKa = 4.1DD115 pKa = 3.96EE116 pKa = 5.31EE117 pKa = 4.53SLEE120 pKa = 4.03MEE122 pKa = 4.31EE123 pKa = 4.08YY124 pKa = 10.63GYY126 pKa = 11.17DD127 pKa = 3.38GLEE130 pKa = 3.98QVEE133 pKa = 4.33DD134 pKa = 3.9EE135 pKa = 4.35SRR137 pKa = 11.84SVGGMGDD144 pKa = 4.95DD145 pKa = 3.57IDD147 pKa = 5.91GPDD150 pKa = 3.7LEE152 pKa = 4.7EE153 pKa = 5.55DD154 pKa = 3.73EE155 pKa = 5.35PEE157 pKa = 4.63LGLEE161 pKa = 4.34DD162 pKa = 5.36GEE164 pKa = 5.74DD165 pKa = 3.43EE166 pKa = 5.4VFPQDD171 pKa = 3.3EE172 pKa = 5.02LVWGDD177 pKa = 3.58YY178 pKa = 11.54GEE180 pKa = 4.21LL181 pKa = 3.63

Molecular weight: 20.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C3P400|A0A0C3P400_PISTI Uncharacterized protein (Fragment) OS=Pisolithus tinctorius Marx 270 OX=870435 GN=M404DRAFT_70026 PE=4 SV=1
MM1 pKa = 7.6AWRR4 pKa = 11.84FPRR7 pKa = 11.84VSWRR11 pKa = 11.84FPSMAWRR18 pKa = 11.84FPRR21 pKa = 11.84VSWRR25 pKa = 11.84FPNIAWGFPSIPWGIPRR42 pKa = 11.84VGGRR46 pKa = 11.84IPRR49 pKa = 11.84VSWRR53 pKa = 11.84IHH55 pKa = 6.21SMAQRR60 pKa = 11.84IPRR63 pKa = 11.84VGGRR67 pKa = 11.84FPSIAWGIPKK77 pKa = 10.17VGWRR81 pKa = 11.84FPSIAWRR88 pKa = 11.84IPRR91 pKa = 11.84VSWRR95 pKa = 11.84IPSIPWRR102 pKa = 11.84IPRR105 pKa = 11.84VGGRR109 pKa = 11.84VPGNSSVV116 pKa = 3.07

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22400

9

22409

6502442

49

5097

290.2

32.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.064 ± 0.016

1.768 ± 0.009

5.508 ± 0.013

6.022 ± 0.021

3.622 ± 0.011

6.151 ± 0.016

2.924 ± 0.009

4.819 ± 0.012

4.57 ± 0.017

9.309 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.007

3.45 ± 0.009

6.247 ± 0.021

3.866 ± 0.01

6.22 ± 0.018

8.537 ± 0.025

6.002 ± 0.013

6.499 ± 0.014

1.561 ± 0.007

2.612 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski