Mycobacterium phage Chadwick

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2CN50|A0A0K2CN50_9CAUD Uncharacterized protein OS=Mycobacterium phage Chadwick OX=1698366 GN=SEA_CHADWICK_86 PE=4 SV=1
MM1 pKa = 6.63TAAVQAEE8 pKa = 4.18PFLIRR13 pKa = 11.84EE14 pKa = 4.39GSSLRR19 pKa = 11.84TQDD22 pKa = 3.27EE23 pKa = 4.06LDD25 pKa = 3.81YY26 pKa = 11.42IFVQDD31 pKa = 4.14VEE33 pKa = 5.79QIAQWSHH40 pKa = 6.2PDD42 pKa = 3.4DD43 pKa = 3.85YY44 pKa = 11.66EE45 pKa = 4.0RR46 pKa = 11.84ASDD49 pKa = 3.96YY50 pKa = 11.36LCEE53 pKa = 5.03AEE55 pKa = 4.63LAIEE59 pKa = 5.04EE60 pKa = 4.04VDD62 pKa = 3.17DD63 pKa = 4.01WSRR66 pKa = 11.84EE67 pKa = 4.04DD68 pKa = 3.78LQHH71 pKa = 6.5IAASAEE77 pKa = 3.8GLLNDD82 pKa = 3.32MGFYY86 pKa = 10.85VEE88 pKa = 4.66WNDD91 pKa = 3.71GYY93 pKa = 11.5VIYY96 pKa = 10.19RR97 pKa = 11.84DD98 pKa = 3.79PDD100 pKa = 3.49GDD102 pKa = 4.06GPFPNGAPWKK112 pKa = 9.74VV113 pKa = 2.97

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CN08|A0A0K2CN08_9CAUD Uncharacterized protein OS=Mycobacterium phage Chadwick OX=1698366 GN=SEA_CHADWICK_36 PE=4 SV=1
MM1 pKa = 7.56SAPGWYY7 pKa = 8.48PDD9 pKa = 3.77PAGSGGQRR17 pKa = 11.84YY18 pKa = 8.1WDD20 pKa = 3.72GQRR23 pKa = 11.84WAPQAVHH30 pKa = 6.38AQQVVTGPNHH40 pKa = 5.68VLHH43 pKa = 7.22LILTILTFWFFGGWIWVWLVVALSNKK69 pKa = 9.31KK70 pKa = 10.03RR71 pKa = 11.84IQVVHH76 pKa = 6.56RR77 pKa = 4.27

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

15255

26

937

173.4

19.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.817 ± 0.321

0.898 ± 0.13

6.935 ± 0.197

7.368 ± 0.365

3.343 ± 0.172

8.253 ± 0.432

1.953 ± 0.148

5.356 ± 0.18

4.739 ± 0.253

8.253 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.113

3.441 ± 0.167

5.139 ± 0.29

3.376 ± 0.172

6.142 ± 0.293

5.755 ± 0.211

5.808 ± 0.195

7.198 ± 0.209

2.026 ± 0.146

2.963 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski