Staphylococcus phage phiSa2wa_st121mssa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; Staphylococcus virus st121mssa

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PF33|A0A2I6PF33_9CAUD Major tail protein OS=Staphylococcus phage phiSa2wa_st121mssa OX=2060948 GN=mtp PE=4 SV=1
MM1 pKa = 7.63GGLTMATQRR10 pKa = 11.84QVEE13 pKa = 4.5YY14 pKa = 11.4VMSLQEE20 pKa = 3.92QLEE23 pKa = 4.35LEE25 pKa = 4.45DD26 pKa = 3.95CEE28 pKa = 5.56KK29 pKa = 10.67YY30 pKa = 9.42TDD32 pKa = 3.83EE33 pKa = 4.01QVKK36 pKa = 10.4AMSHH40 pKa = 6.03KK41 pKa = 10.06EE42 pKa = 3.72VSNVIEE48 pKa = 4.41NYY50 pKa = 9.42KK51 pKa = 10.77ASISNEE57 pKa = 3.8EE58 pKa = 4.63LYY60 pKa = 10.85DD61 pKa = 3.48EE62 pKa = 4.93CMSFGLPNCC71 pKa = 4.51

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PEZ9|A0A2I6PEZ9_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa2wa_st121mssa OX=2060948 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84NFLMFLAIIIFLSLFILLTSFFLIIRR28 pKa = 11.84NFHH31 pKa = 6.64IIVKK35 pKa = 9.24FFTKK39 pKa = 8.97KK40 pKa = 8.72TYY42 pKa = 11.09LMLTIRR48 pKa = 11.84KK49 pKa = 8.53FRR51 pKa = 11.84IILGLRR57 pKa = 11.84KK58 pKa = 9.6AVTTT62 pKa = 4.25

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14467

29

2074

222.6

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.737 ± 0.581

0.491 ± 0.09

6.159 ± 0.263

7.534 ± 0.426

3.954 ± 0.228

6.083 ± 0.516

1.818 ± 0.114

7.182 ± 0.325

9.864 ± 0.485

8.26 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.124

6.511 ± 0.275

2.703 ± 0.205

3.608 ± 0.131

3.968 ± 0.223

6.553 ± 0.295

5.675 ± 0.237

5.668 ± 0.214

1.258 ± 0.147

4.313 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski