Thraustotheca clavata

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Thraustotheca

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W0A7G6|A0A1W0A7G6_9STRA Alpha-carbonic anhydrase domain-containing protein (Fragment) OS=Thraustotheca clavata OX=74557 GN=THRCLA_20415 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 4.58EE3 pKa = 5.39NGNQDD8 pKa = 3.93LLIEE12 pKa = 4.26EE13 pKa = 4.99EE14 pKa = 4.29EE15 pKa = 4.56MISADD20 pKa = 3.57LGGGSHH26 pKa = 6.68EE27 pKa = 4.29EE28 pKa = 4.08NKK30 pKa = 10.33LDD32 pKa = 3.58EE33 pKa = 4.64AMGVIQDD40 pKa = 3.78VLIDD44 pKa = 3.76PEE46 pKa = 4.7FVEE49 pKa = 4.77LQNSFCEE56 pKa = 4.05QHH58 pKa = 6.66CEE60 pKa = 4.06YY61 pKa = 10.89FDD63 pKa = 5.26DD64 pKa = 4.28EE65 pKa = 4.99SEE67 pKa = 4.17NKK69 pKa = 9.82LIYY72 pKa = 9.45MDD74 pKa = 4.75LFKK77 pKa = 10.82EE78 pKa = 4.1YY79 pKa = 10.79TNLIEE84 pKa = 5.26NYY86 pKa = 10.42LEE88 pKa = 4.1TRR90 pKa = 11.84LKK92 pKa = 10.86SAIDD96 pKa = 3.95DD97 pKa = 3.95FSMDD101 pKa = 4.41EE102 pKa = 4.41LCSMIQEE109 pKa = 5.09HH110 pKa = 6.61EE111 pKa = 4.37DD112 pKa = 3.58EE113 pKa = 4.42MTGDD117 pKa = 3.91VIDD120 pKa = 4.48VLMSCADD127 pKa = 3.32FDD129 pKa = 4.18EE130 pKa = 5.66FKK132 pKa = 11.41SLMLSFKK139 pKa = 10.5SQQTMLFQIQGDD151 pKa = 4.18SLVCGG156 pKa = 3.91

Molecular weight:
17.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1V9ZYT9|A0A1V9ZYT9_9STRA GTP-binding nuclear protein OS=Thraustotheca clavata OX=74557 GN=THRCLA_04724 PE=3 SV=1
MM1 pKa = 7.37NFFIYY6 pKa = 10.55SLGVDD11 pKa = 3.54TTNQSSKK18 pKa = 11.01VNDD21 pKa = 4.01SIQFSFPKK29 pKa = 10.05CQRR32 pKa = 11.84FQVKK36 pKa = 10.32RR37 pKa = 11.84ITEE40 pKa = 4.42RR41 pKa = 11.84KK42 pKa = 9.14QSSNQINPGIYY53 pKa = 9.52VVRR56 pKa = 11.84NTHH59 pKa = 5.45TGHH62 pKa = 6.35TFFGSTWDD70 pKa = 3.75LEE72 pKa = 4.38NAKK75 pKa = 9.96KK76 pKa = 10.59QNFQDD81 pKa = 3.77LQLGRR86 pKa = 11.84HH87 pKa = 5.92EE88 pKa = 4.02NRR90 pKa = 11.84AMVRR94 pKa = 11.84AVQVYY99 pKa = 8.83GFKK102 pKa = 8.36TTTFQILQTIPTPKK116 pKa = 9.47EE117 pKa = 3.28FHH119 pKa = 6.21FRR121 pKa = 11.84EE122 pKa = 4.37LEE124 pKa = 3.92NTLTQRR130 pKa = 11.84LAFFIAKK137 pKa = 8.77AHH139 pKa = 6.08KK140 pKa = 8.88VHH142 pKa = 6.51ARR144 pKa = 11.84KK145 pKa = 9.7LFNQWQLQYY154 pKa = 10.32FTFAWPMWKK163 pKa = 9.95AHH165 pKa = 4.87VVQTKK170 pKa = 9.56QIEE173 pKa = 4.35YY174 pKa = 9.14EE175 pKa = 3.86AATIEE180 pKa = 4.17IQRR183 pKa = 11.84VWRR186 pKa = 11.84GLKK189 pKa = 10.02GRR191 pKa = 11.84LRR193 pKa = 11.84AIDD196 pKa = 3.6QRR198 pKa = 11.84HH199 pKa = 5.42FCCATLIQWMVRR211 pKa = 11.84GFLAHH216 pKa = 7.04RR217 pKa = 11.84CLLRR221 pKa = 11.84LRR223 pKa = 11.84LNRR226 pKa = 11.84KK227 pKa = 8.87ARR229 pKa = 11.84TIQHH233 pKa = 4.31VAKK236 pKa = 10.51RR237 pKa = 11.84FLQLALYY244 pKa = 9.35KK245 pKa = 9.9RR246 pKa = 11.84WKK248 pKa = 10.52AGALLIQRR256 pKa = 11.84VWRR259 pKa = 11.84GVRR262 pKa = 11.84DD263 pKa = 3.39RR264 pKa = 11.84RR265 pKa = 11.84YY266 pKa = 8.72VNQRR270 pKa = 11.84RR271 pKa = 11.84ILKK274 pKa = 8.61QQNQAAFCLQRR285 pKa = 11.84NLRR288 pKa = 11.84LHH290 pKa = 6.45QIHH293 pKa = 5.94QKK295 pKa = 7.56WLRR298 pKa = 11.84QRR300 pKa = 11.84RR301 pKa = 11.84HH302 pKa = 4.71QLHH305 pKa = 5.87TKK307 pKa = 10.14ASIVIQSNWRR317 pKa = 11.84GFHH320 pKa = 5.75GRR322 pKa = 11.84QIALAKK328 pKa = 10.59NILRR332 pKa = 11.84KK333 pKa = 7.55QQRR336 pKa = 11.84AKK338 pKa = 10.5NAAARR343 pKa = 11.84LIQEE347 pKa = 4.69SYY349 pKa = 11.13HH350 pKa = 6.12EE351 pKa = 4.11YY352 pKa = 9.92QVKK355 pKa = 10.13KK356 pKa = 9.82FQNAAQTIYY365 pKa = 9.6CQKK368 pKa = 10.49RR369 pKa = 11.84MAVRR373 pKa = 11.84LSVYY377 pKa = 7.33WHH379 pKa = 6.12NFIARR384 pKa = 11.84KK385 pKa = 9.33FGWAALQIHH394 pKa = 7.0LEE396 pKa = 3.96HH397 pKa = 7.19KK398 pKa = 9.97MAIKK402 pKa = 9.83LQQWAHH408 pKa = 4.62QRR410 pKa = 11.84LSIKK414 pKa = 10.15RR415 pKa = 11.84IHH417 pKa = 6.95RR418 pKa = 11.84GCLQCKK424 pKa = 9.42RR425 pKa = 11.84IRR427 pKa = 11.84RR428 pKa = 11.84AICIQRR434 pKa = 11.84NVRR437 pKa = 11.84GFLARR442 pKa = 11.84HH443 pKa = 6.13IYY445 pKa = 8.57VPRR448 pKa = 11.84VKK450 pKa = 10.26RR451 pKa = 11.84EE452 pKa = 3.78RR453 pKa = 11.84EE454 pKa = 3.78LKK456 pKa = 9.95IACNYY461 pKa = 7.95IGRR464 pKa = 11.84WYY466 pKa = 10.68RR467 pKa = 11.84SLKK470 pKa = 7.36WHH472 pKa = 6.8RR473 pKa = 11.84LVCFLFRR480 pKa = 11.84TKK482 pKa = 10.55VATQIQALFRR492 pKa = 11.84GYY494 pKa = 9.58VVWKK498 pKa = 10.07HH499 pKa = 4.42YY500 pKa = 8.54QEE502 pKa = 5.9CKK504 pKa = 10.4DD505 pKa = 3.03AWKK508 pKa = 10.37EE509 pKa = 3.68EE510 pKa = 4.12KK511 pKa = 10.37AALTIQKK518 pKa = 9.85QYY520 pKa = 10.35RR521 pKa = 11.84KK522 pKa = 9.42HH523 pKa = 6.13IAMLKK528 pKa = 7.76FQHH531 pKa = 6.07RR532 pKa = 11.84VAVLKK537 pKa = 10.94AGACAEE543 pKa = 4.25CGEE546 pKa = 4.88EE547 pKa = 4.17IATLYY552 pKa = 11.31SMSFEE557 pKa = 4.18MEE559 pKa = 4.13LCHH562 pKa = 6.84GCWSTIKK569 pKa = 10.62GFTGTTDD576 pKa = 3.66DD577 pKa = 3.99KK578 pKa = 11.4SIPIDD583 pKa = 3.31QYY585 pKa = 10.98RR586 pKa = 11.84LEE588 pKa = 4.54RR589 pKa = 11.84LLATKK594 pKa = 10.29LAAVYY599 pKa = 9.86RR600 pKa = 11.84GYY602 pKa = 11.07AARR605 pKa = 11.84LLYY608 pKa = 9.94KK609 pKa = 9.96VPCCSNCTTASRR621 pKa = 11.84LICLEE626 pKa = 4.24CPLILCHH633 pKa = 6.34SCSATVHH640 pKa = 5.58TLPYY644 pKa = 10.54NIFHH648 pKa = 6.6HH649 pKa = 6.25PWRR652 pKa = 11.84LAEE655 pKa = 4.0HH656 pKa = 6.17EE657 pKa = 4.43RR658 pKa = 11.84RR659 pKa = 11.84LAAAIRR665 pKa = 11.84LQTRR669 pKa = 11.84IRR671 pKa = 11.84CFLEE675 pKa = 4.69RR676 pKa = 11.84NTLQQLRR683 pKa = 11.84DD684 pKa = 3.5AMLLEE689 pKa = 4.72VIIKK693 pKa = 8.81VQGWWRR699 pKa = 11.84TRR701 pKa = 11.84YY702 pKa = 9.29ARR704 pKa = 11.84HH705 pKa = 6.38LARR708 pKa = 11.84VLQEE712 pKa = 3.31QTRR715 pKa = 11.84RR716 pKa = 11.84YY717 pKa = 7.02EE718 pKa = 5.17AKK720 pKa = 9.77RR721 pKa = 11.84QWAAGIIQRR730 pKa = 11.84NYY732 pKa = 8.93RR733 pKa = 11.84GHH735 pKa = 6.17MGCLIAKK742 pKa = 7.65EE743 pKa = 3.96EE744 pKa = 3.85RR745 pKa = 11.84RR746 pKa = 11.84YY747 pKa = 9.38RR748 pKa = 11.84QAVITIQSQFRR759 pKa = 11.84AAIARR764 pKa = 11.84RR765 pKa = 11.84KK766 pKa = 8.62VAQEE770 pKa = 3.59RR771 pKa = 11.84QRR773 pKa = 11.84RR774 pKa = 11.84LHH776 pKa = 6.23IRR778 pKa = 11.84RR779 pKa = 11.84QAAALVLQCAIRR791 pKa = 11.84QYY793 pKa = 10.63FARR796 pKa = 11.84QDD798 pKa = 3.19LRR800 pKa = 11.84RR801 pKa = 11.84RR802 pKa = 11.84RR803 pKa = 11.84EE804 pKa = 3.42FFAALRR810 pKa = 11.84IQCTIRR816 pKa = 11.84VYY818 pKa = 10.32LAKK821 pKa = 10.18QVLLALQIEE830 pKa = 4.69YY831 pKa = 10.3EE832 pKa = 4.36RR833 pKa = 11.84KK834 pKa = 8.18FQLAIEE840 pKa = 4.1QVKK843 pKa = 8.42YY844 pKa = 8.42QRR846 pKa = 11.84KK847 pKa = 7.3VRR849 pKa = 11.84AVIRR853 pKa = 11.84IQSQYY858 pKa = 9.65RR859 pKa = 11.84RR860 pKa = 11.84RR861 pKa = 11.84RR862 pKa = 11.84DD863 pKa = 3.24FRR865 pKa = 11.84VAVAKK870 pKa = 10.57RR871 pKa = 11.84LANTKK876 pKa = 9.7ARR878 pKa = 11.84RR879 pKa = 11.84EE880 pKa = 3.82HH881 pKa = 6.22WEE883 pKa = 4.12RR884 pKa = 11.84VCLLMEE890 pKa = 4.44KK891 pKa = 10.32SSAKK895 pKa = 10.24RR896 pKa = 11.84LQQWYY901 pKa = 9.08RR902 pKa = 11.84RR903 pKa = 11.84RR904 pKa = 11.84IEE906 pKa = 4.17RR907 pKa = 11.84LNALATRR914 pKa = 11.84IQCMVRR920 pKa = 11.84CHH922 pKa = 4.7VARR925 pKa = 11.84RR926 pKa = 11.84VLGRR930 pKa = 11.84HH931 pKa = 5.39RR932 pKa = 11.84ANAIEE937 pKa = 4.32TKK939 pKa = 9.78RR940 pKa = 11.84VRR942 pKa = 11.84AAVCLQCFSRR952 pKa = 11.84RR953 pKa = 11.84IIARR957 pKa = 11.84RR958 pKa = 11.84IVNGLRR964 pKa = 11.84AEE966 pKa = 4.2KK967 pKa = 10.21QVVEE971 pKa = 4.28NLPKK975 pKa = 9.35EE976 pKa = 4.26DD977 pKa = 3.76AWIEE981 pKa = 4.08CLDD984 pKa = 3.7EE985 pKa = 4.74SSGYY989 pKa = 8.23TYY991 pKa = 10.86YY992 pKa = 11.27YY993 pKa = 10.54NPQTQATTWTKK1004 pKa = 10.76PNNTVNEE1011 pKa = 4.27VVEE1014 pKa = 4.43KK1015 pKa = 10.98VDD1017 pKa = 5.82AEE1019 pKa = 4.19PQDD1022 pKa = 4.27TEE1024 pKa = 5.8WIACWDD1030 pKa = 3.96DD1031 pKa = 3.96SYY1033 pKa = 11.47QAYY1036 pKa = 10.47YY1037 pKa = 10.9FMNKK1041 pKa = 7.24YY1042 pKa = 9.09TGDD1045 pKa = 4.08TKK1047 pKa = 10.87WEE1049 pKa = 4.43DD1050 pKa = 3.54PTQASYY1056 pKa = 9.78EE1057 pKa = 4.02WYY1059 pKa = 9.9YY1060 pKa = 11.33DD1061 pKa = 3.4EE1062 pKa = 5.35NGQVVYY1068 pKa = 10.02YY1069 pKa = 7.52QQPEE1073 pKa = 4.02NQEE1076 pKa = 3.78

Molecular weight:
128.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13171

0

13171

6794705

49

10056

515.9

57.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.671 ± 0.017

1.883 ± 0.012

5.188 ± 0.016

5.917 ± 0.027

4.09 ± 0.017

5.271 ± 0.024

2.622 ± 0.01

5.902 ± 0.015

5.56 ± 0.022

9.93 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.009

4.49 ± 0.012

4.718 ± 0.022

4.371 ± 0.014

4.871 ± 0.017

7.652 ± 0.025

6.142 ± 0.02

6.579 ± 0.014

1.309 ± 0.008

3.21 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski