Escherichia phage vB_EcoS_IME347

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Badaguanvirus; Escherichia virus IME347

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GSB0|A0A2S1GSB0_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_IME347 OX=2496546 PE=4 SV=1
MM1 pKa = 7.39TNAPCKK7 pKa = 10.91NNDD10 pKa = 3.1DD11 pKa = 4.38NFWHH15 pKa = 7.0RR16 pKa = 11.84FLTAQDD22 pKa = 3.35AGLNRR27 pKa = 11.84EE28 pKa = 4.04YY29 pKa = 10.32CIKK32 pKa = 10.52VAYY35 pKa = 9.7QDD37 pKa = 3.67LTLEE41 pKa = 4.14QALGEE46 pKa = 3.98MDD48 pKa = 3.66MDD50 pKa = 4.57AEE52 pKa = 4.99SEE54 pKa = 4.0FDD56 pKa = 3.77PNFVMTAEE64 pKa = 5.08DD65 pKa = 3.82DD66 pKa = 4.09SEE68 pKa = 4.39PGKK71 pKa = 10.56DD72 pKa = 3.6YY73 pKa = 11.19IPWW76 pKa = 4.04

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GS97|A0A2S1GS97_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_IME347 OX=2496546 PE=4 SV=1
MM1 pKa = 6.9TIKK4 pKa = 10.59RR5 pKa = 11.84IAEE8 pKa = 4.03STGEE12 pKa = 3.51IDD14 pKa = 4.61RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 6.15LNGNNGTRR25 pKa = 11.84RR26 pKa = 11.84GKK28 pKa = 9.79DD29 pKa = 2.9VKK31 pKa = 10.17PRR33 pKa = 11.84KK34 pKa = 9.52RR35 pKa = 11.84CGFYY39 pKa = 10.09ILKK42 pKa = 10.56DD43 pKa = 3.52EE44 pKa = 4.28VRR46 pKa = 11.84AGLRR50 pKa = 11.84ARR52 pKa = 11.84LDD54 pKa = 3.56ALIEE58 pKa = 4.65FYY60 pKa = 10.98GSPSATAKK68 pKa = 10.32ALKK71 pKa = 8.85VTNQNVNEE79 pKa = 4.1WKK81 pKa = 9.72VRR83 pKa = 11.84GMISWQGAEE92 pKa = 3.99AAHH95 pKa = 6.1RR96 pKa = 11.84AYY98 pKa = 10.32RR99 pKa = 11.84RR100 pKa = 11.84QGCTGYY106 pKa = 10.08RR107 pKa = 11.84ASWLRR112 pKa = 11.84FDD114 pKa = 5.9LKK116 pKa = 10.76FDD118 pKa = 4.39SNGKK122 pKa = 9.34CLEE125 pKa = 4.13KK126 pKa = 10.33RR127 pKa = 11.84CKK129 pKa = 9.4NRR131 pKa = 11.84KK132 pKa = 7.53FMRR135 pKa = 11.84VVRR138 pKa = 11.84KK139 pKa = 10.61DD140 pKa = 3.45EE141 pKa = 4.19VEE143 pKa = 4.28STNSS147 pKa = 3.05

Molecular weight: 16.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15125

41

1193

173.9

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.575 ± 0.387

1.362 ± 0.177

6.003 ± 0.193

7.048 ± 0.279

4.231 ± 0.206

7.65 ± 0.236

1.831 ± 0.2

6.175 ± 0.202

6.902 ± 0.335

6.539 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.14 ± 0.143

4.8 ± 0.334

3.345 ± 0.189

3.861 ± 0.262

5.084 ± 0.23

5.944 ± 0.251

5.296 ± 0.236

7.14 ± 0.319

1.507 ± 0.096

3.564 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski