Elizabethkingia phage TCUEAP1

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G5XN72|A0A6G5XN72_9VIRU Uncharacterized protein OS=Elizabethkingia phage TCUEAP1 OX=2686291 PE=4 SV=1
MM1 pKa = 7.41NNSIYY6 pKa = 10.44VIPSLDD12 pKa = 4.06LIAKK16 pKa = 9.55EE17 pKa = 4.43EE18 pKa = 4.35IINDD22 pKa = 3.41LRR24 pKa = 11.84FDD26 pKa = 3.72AFEE29 pKa = 4.0NAQKK33 pKa = 10.52DD34 pKa = 3.55RR35 pKa = 11.84HH36 pKa = 5.87AVLSVIYY43 pKa = 9.03EE44 pKa = 4.28AYY46 pKa = 9.74SAPTITLANQVYY58 pKa = 8.05TVFDD62 pKa = 3.81FDD64 pKa = 4.36GYY66 pKa = 11.6DD67 pKa = 3.3MQEE70 pKa = 4.0VKK72 pKa = 10.89DD73 pKa = 4.2VIDD76 pKa = 4.22TIGVYY81 pKa = 10.08YY82 pKa = 8.02QTII85 pKa = 3.35

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G5XNM9|A0A6G5XNM9_9VIRU Uncharacterized protein OS=Elizabethkingia phage TCUEAP1 OX=2686291 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.89PTIDD6 pKa = 3.45NLIYY10 pKa = 10.4NPKK13 pKa = 9.38RR14 pKa = 11.84KK15 pKa = 9.39QFTGVLHH22 pKa = 5.31YY23 pKa = 10.8QNIRR27 pKa = 11.84LVIKK31 pKa = 10.52PNGQSFAIKK40 pKa = 10.36NGVVLLRR47 pKa = 11.84EE48 pKa = 4.02HH49 pKa = 6.81NVYY52 pKa = 9.89KK53 pKa = 9.32WAKK56 pKa = 8.06RR57 pKa = 11.84RR58 pKa = 11.84IGIRR62 pKa = 11.84LYY64 pKa = 10.67KK65 pKa = 10.52RR66 pKa = 11.84NFKK69 pKa = 10.77SRR71 pKa = 11.84LTNHH75 pKa = 5.9YY76 pKa = 8.55PLAFVTHH83 pKa = 6.33NEE85 pKa = 3.47IKK87 pKa = 9.86MMVWRR92 pKa = 11.84GQIRR96 pKa = 11.84RR97 pKa = 11.84QSRR100 pKa = 11.84MIKK103 pKa = 10.32LNWRR107 pKa = 11.84TMFTKK112 pKa = 10.35SGSLFGGNIVPQQITKK128 pKa = 9.64LQEE131 pKa = 3.78SSIVRR136 pKa = 11.84QEE138 pKa = 3.86VKK140 pKa = 10.93NSVVCNTNTRR150 pKa = 11.84VLNDD154 pKa = 3.02ILKK157 pKa = 10.35YY158 pKa = 10.74EE159 pKa = 4.04NLKK162 pKa = 10.75

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15315

49

1465

209.8

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.365 ± 0.617

0.947 ± 0.15

6.477 ± 0.264

7.15 ± 0.335

4.133 ± 0.155

6.386 ± 0.249

1.567 ± 0.204

7.535 ± 0.296

7.705 ± 0.349

7.946 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.117

5.975 ± 0.314

3.049 ± 0.208

3.369 ± 0.253

4.447 ± 0.235

5.563 ± 0.194

5.916 ± 0.274

6.327 ± 0.225

1.156 ± 0.153

4.636 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski