Parabacteroides johnsonii CL02T12C29

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides johnsonii

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K5Y9I6|K5Y9I6_9BACT PfkB domain-containing protein OS=Parabacteroides johnsonii CL02T12C29 OX=999419 GN=HMPREF1077_01852 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 4.97NLRR5 pKa = 11.84NLSRR9 pKa = 11.84WGMFAFLSVALCTSFVGCSDD29 pKa = 5.24DD30 pKa = 5.71DD31 pKa = 4.06PDD33 pKa = 4.12YY34 pKa = 11.93SNVTPPTVSVSHH46 pKa = 6.57SISGRR51 pKa = 11.84VTGMDD56 pKa = 3.61GNGLSATVSMNGEE69 pKa = 4.23STQTGADD76 pKa = 3.41GTFTFDD82 pKa = 4.93DD83 pKa = 3.97VDD85 pKa = 3.84AGSYY89 pKa = 9.32TLTASAPGKK98 pKa = 8.92QSKK101 pKa = 7.79EE102 pKa = 4.09TTVTVAEE109 pKa = 4.54SGSGANVVWNVSLPNEE125 pKa = 4.02GTTIEE130 pKa = 4.23VAANGDD136 pKa = 3.45TEE138 pKa = 5.54ANVTSEE144 pKa = 4.29TIEE147 pKa = 4.38GNDD150 pKa = 3.25EE151 pKa = 4.09GAVTVAVTVPEE162 pKa = 4.27TAEE165 pKa = 4.12LPAGSSIVVTPLYY178 pKa = 10.43TLDD181 pKa = 3.42EE182 pKa = 4.69AEE184 pKa = 4.44ANTRR188 pKa = 11.84ATSRR192 pKa = 11.84AAEE195 pKa = 4.08SVMLIGTSVACTDD208 pKa = 3.59ANATLSSPIEE218 pKa = 3.72LAYY221 pKa = 10.67DD222 pKa = 3.59VDD224 pKa = 4.43AEE226 pKa = 4.44VAQSITAQKK235 pKa = 9.47YY236 pKa = 8.92VNGQWVDD243 pKa = 3.03AEE245 pKa = 4.43YY246 pKa = 9.75TVEE249 pKa = 4.23GGQVIVFADD258 pKa = 3.37QFTSYY263 pKa = 10.01TLLFGADD270 pKa = 3.62VTSSSTSTPLAFEE283 pKa = 3.63QDD285 pKa = 3.31LWDD288 pKa = 3.83NLYY291 pKa = 11.1GSGDD295 pKa = 3.61MTVGSASFTYY305 pKa = 10.36HH306 pKa = 6.63IGTEE310 pKa = 4.13ITSSGTSRR318 pKa = 11.84ITAYY322 pKa = 10.64LIEE325 pKa = 5.08ILARR329 pKa = 11.84IAGAGVTTATGTYY342 pKa = 8.51PINVTLPVGTALHH355 pKa = 5.82VAGTQQVTTLTVSALNRR372 pKa = 11.84SVSGRR377 pKa = 11.84QYY379 pKa = 11.17GDD381 pKa = 3.19VAVVTTSYY389 pKa = 11.08NRR391 pKa = 11.84NHH393 pKa = 6.77NGGTSDD399 pKa = 3.22NN400 pKa = 3.97

Molecular weight: 41.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6A1X0|K6A1X0_9BACT RNA polymerase sigma-70 factor expansion family 1 OS=Parabacteroides johnsonii CL02T12C29 OX=999419 GN=HMPREF1077_02163 PE=3 SV=1
MM1 pKa = 7.55GFRR4 pKa = 11.84VNRR7 pKa = 11.84RR8 pKa = 11.84TLLLIAGCVWIIAGINILRR27 pKa = 11.84IGIVTWLNDD36 pKa = 2.91AHH38 pKa = 6.52YY39 pKa = 9.65WLFKK43 pKa = 9.85VGEE46 pKa = 4.45AIVVFLLFFQFVFRR60 pKa = 11.84RR61 pKa = 11.84LFIKK65 pKa = 10.19HH66 pKa = 5.93SEE68 pKa = 4.22RR69 pKa = 11.84ISQKK73 pKa = 10.2GEE75 pKa = 3.89KK76 pKa = 9.4NCPFSFFDD84 pKa = 3.48VKK86 pKa = 10.74GWIVMIFMITFGITIRR102 pKa = 11.84HH103 pKa = 5.37FQLLPNSFISVFYY116 pKa = 10.17TGLSSALIITGILFLIRR133 pKa = 11.84AYY135 pKa = 10.61SHH137 pKa = 6.03TFNRR141 pKa = 11.84SS142 pKa = 2.53

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3736

0

3736

1374269

22

3256

367.8

41.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.07 ± 0.041

1.186 ± 0.015

5.599 ± 0.024

6.639 ± 0.035

4.646 ± 0.024

6.915 ± 0.038

1.825 ± 0.017

6.827 ± 0.037

6.611 ± 0.031

9.205 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.017

4.982 ± 0.036

3.838 ± 0.024

3.395 ± 0.02

4.706 ± 0.03

6.057 ± 0.03

5.56 ± 0.024

6.565 ± 0.032

1.263 ± 0.018

4.404 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski