Sunn-hemp mosaic virus (SHMV) (TMV strain cowpea)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03585|MVP_SHMV Movement protein OS=Sunn-hemp mosaic virus OX=12240 GN=MP PE=3 SV=1
MM1 pKa = 7.8AYY3 pKa = 10.35SIPTPSQLVYY13 pKa = 9.43FTEE16 pKa = 4.52NYY18 pKa = 10.25ADD20 pKa = 4.24YY21 pKa = 10.83IPFVNRR27 pKa = 11.84LINARR32 pKa = 11.84SNSFQTQSGRR42 pKa = 11.84DD43 pKa = 3.71EE44 pKa = 4.06LRR46 pKa = 11.84EE47 pKa = 3.87ILIKK51 pKa = 10.83SQVSVVSPISRR62 pKa = 11.84FPAEE66 pKa = 3.76PAYY69 pKa = 10.4YY70 pKa = 9.59IYY72 pKa = 10.95LRR74 pKa = 11.84DD75 pKa = 3.67PSISTVYY82 pKa = 9.51TALLQSTDD90 pKa = 2.58TRR92 pKa = 11.84NRR94 pKa = 11.84VIEE97 pKa = 4.2VEE99 pKa = 3.75NSTNVTTAEE108 pKa = 3.73QLNAVRR114 pKa = 11.84RR115 pKa = 11.84TDD117 pKa = 3.53DD118 pKa = 3.29ASTAIHH124 pKa = 6.63NNLEE128 pKa = 4.1QLLSLLTNGTGVFNRR143 pKa = 11.84TSFEE147 pKa = 4.24SASGLTWLVTTTPRR161 pKa = 11.84TAA163 pKa = 3.4

Molecular weight: 18.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P89202|RDRP_SHMV Replicase large subunit OS=Sunn-hemp mosaic virus OX=12240 PE=2 SV=2
MM1 pKa = 7.69SEE3 pKa = 3.81VSKK6 pKa = 10.83ISTLLAPEE14 pKa = 4.51KK15 pKa = 10.13FVKK18 pKa = 10.67LSVSDD23 pKa = 3.52KK24 pKa = 10.58FKK26 pKa = 10.06WKK28 pKa = 10.42APSRR32 pKa = 11.84VCSIVQSDD40 pKa = 4.8TISMTANGRR49 pKa = 11.84SLFTFDD55 pKa = 4.13VLKK58 pKa = 10.92DD59 pKa = 3.75VLKK62 pKa = 10.65HH63 pKa = 5.86AEE65 pKa = 4.06EE66 pKa = 4.14YY67 pKa = 10.43TYY69 pKa = 11.52VDD71 pKa = 2.99VLGVVLSGQWLLPKK85 pKa = 8.94GTPGSAEE92 pKa = 4.0IILLDD97 pKa = 3.96SRR99 pKa = 11.84LKK101 pKa = 11.04GKK103 pKa = 10.68ASVLAVFNCRR113 pKa = 11.84AATQEE118 pKa = 4.02FQFLISPGYY127 pKa = 10.79SLTCADD133 pKa = 3.92ALKK136 pKa = 10.68KK137 pKa = 10.39PFEE140 pKa = 4.26ISCNVIDD147 pKa = 5.87LPVKK151 pKa = 10.82DD152 pKa = 4.38GFTPLSVEE160 pKa = 4.13IACLVQFSNCVITRR174 pKa = 11.84SLTMKK179 pKa = 10.46LKK181 pKa = 10.33EE182 pKa = 4.18NPATRR187 pKa = 11.84TFSAEE192 pKa = 4.09EE193 pKa = 3.79VDD195 pKa = 4.06EE196 pKa = 4.75LLGSMTTLRR205 pKa = 11.84SIEE208 pKa = 3.91GLRR211 pKa = 11.84KK212 pKa = 9.72KK213 pKa = 10.57KK214 pKa = 10.34EE215 pKa = 4.06PNDD218 pKa = 3.42VVQGHH223 pKa = 7.03LSAEE227 pKa = 4.15YY228 pKa = 10.13DD229 pKa = 3.45VKK231 pKa = 11.04RR232 pKa = 11.84SVKK235 pKa = 8.92RR236 pKa = 11.84TKK238 pKa = 10.71SEE240 pKa = 3.67NTPGKK245 pKa = 10.15RR246 pKa = 11.84RR247 pKa = 11.84VNVDD251 pKa = 3.42SVSLGLGKK259 pKa = 10.6GKK261 pKa = 10.35SVSAKK266 pKa = 10.73NEE268 pKa = 3.86DD269 pKa = 3.94TEE271 pKa = 5.33SVFDD275 pKa = 5.93DD276 pKa = 5.52GILDD280 pKa = 3.69SDD282 pKa = 4.05SS283 pKa = 3.32

Molecular weight: 30.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2075

163

1629

691.7

78.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.639 ± 0.434

2.072 ± 0.511

6.506 ± 0.732

5.687 ± 0.192

5.108 ± 0.552

4.771 ± 0.493

1.928 ± 0.622

5.494 ± 0.336

6.458 ± 1.243

9.783 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.88 ± 0.413

4.819 ± 0.568

4.096 ± 0.177

3.277 ± 0.313

5.446 ± 0.425

7.566 ± 1.892

6.12 ± 1.581

8.193 ± 0.457

1.06 ± 0.195

4.0 ± 0.715
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski