Kockovaella imperatae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cuniculitremaceae; Kockovaella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1ULI1|A0A1Y1ULI1_9TREE Uncharacterized protein OS=Kockovaella imperatae OX=4999 GN=BD324DRAFT_617509 PE=4 SV=1
MM1 pKa = 7.28SACSSPDD8 pKa = 3.45LEE10 pKa = 4.45IVTPALTVEE19 pKa = 3.99GDD21 pKa = 4.23FYY23 pKa = 11.61CNEE26 pKa = 4.14DD27 pKa = 3.35DD28 pKa = 4.57VYY30 pKa = 11.24EE31 pKa = 4.25LGYY34 pKa = 10.47EE35 pKa = 4.13YY36 pKa = 10.7EE37 pKa = 4.4SDD39 pKa = 3.77SEE41 pKa = 4.55SEE43 pKa = 4.44SEE45 pKa = 4.45SEE47 pKa = 3.99SHH49 pKa = 6.87AGVGFDD55 pKa = 4.22LFRR58 pKa = 11.84FPSIGFMPSIGQVLDD73 pKa = 4.39SIEE76 pKa = 4.51CPTVEE81 pKa = 4.33KK82 pKa = 8.45TTAVDD87 pKa = 3.08THH89 pKa = 7.9SFDD92 pKa = 6.02DD93 pKa = 4.11VTTHH97 pKa = 7.98DD98 pKa = 5.43DD99 pKa = 4.98DD100 pKa = 6.8DD101 pKa = 6.86DD102 pKa = 5.15NDD104 pKa = 3.75EE105 pKa = 5.57DD106 pKa = 4.89GDD108 pKa = 3.95PSYY111 pKa = 11.4LEE113 pKa = 3.76EE114 pKa = 4.08NARR117 pKa = 11.84DD118 pKa = 4.15FIGPRR123 pKa = 11.84TLAFQAVDD131 pKa = 3.67EE132 pKa = 4.53SWEE135 pKa = 4.18HH136 pKa = 6.84FIGPHH141 pKa = 4.82SKK143 pKa = 9.69MYY145 pKa = 10.76LEE147 pKa = 4.7WIAYY151 pKa = 9.37NEE153 pKa = 4.1AVDD156 pKa = 3.86AAEE159 pKa = 4.14KK160 pKa = 10.19EE161 pKa = 4.59YY162 pKa = 11.29VFTRR166 pKa = 11.84VMEE169 pKa = 4.52GSEE172 pKa = 3.68WTYY175 pKa = 11.6NEE177 pKa = 4.05SDD179 pKa = 3.98ADD181 pKa = 4.22FIGPKK186 pKa = 7.3TQEE189 pKa = 3.46HH190 pKa = 6.46HH191 pKa = 7.02ALDD194 pKa = 3.95EE195 pKa = 4.58ASTGFIGPVSFGGTLLKK212 pKa = 10.74LLEE215 pKa = 4.39PFIEE219 pKa = 4.49AALAADD225 pKa = 3.76EE226 pKa = 4.61EE227 pKa = 4.74EE228 pKa = 4.53EE229 pKa = 4.78KK230 pKa = 11.18EE231 pKa = 3.99GDD233 pKa = 3.25TSFRR237 pKa = 11.84QEE239 pKa = 3.34GAKK242 pKa = 10.54YY243 pKa = 10.64VDD245 pKa = 4.69AEE247 pKa = 4.29DD248 pKa = 3.68QAEE251 pKa = 4.22MEE253 pKa = 4.33HH254 pKa = 7.17VEE256 pKa = 4.61LIGAEE261 pKa = 3.94SDD263 pKa = 4.08LEE265 pKa = 4.49GEE267 pKa = 4.27GSGWVGCVIAA277 pKa = 5.6

Molecular weight: 30.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1UG68|A0A1Y1UG68_9TREE Uncharacterized protein OS=Kockovaella imperatae OX=4999 GN=BD324DRAFT_629123 PE=4 SV=1
SS1 pKa = 6.16TLRR4 pKa = 11.84ASLIPRR10 pKa = 11.84ITSLLRR16 pKa = 11.84PTLSSPIPSALLPHH30 pKa = 6.85NSPLDD35 pKa = 3.84LGQVRR40 pKa = 11.84FGSKK44 pKa = 10.05GGNTYY49 pKa = 10.38QPSQRR54 pKa = 11.84KK55 pKa = 8.78RR56 pKa = 11.84KK57 pKa = 8.94RR58 pKa = 11.84KK59 pKa = 9.42HH60 pKa = 5.72GFLSRR65 pKa = 11.84IKK67 pKa = 8.35TAKK70 pKa = 9.0GRR72 pKa = 11.84KK73 pKa = 7.91ILTRR77 pKa = 11.84RR78 pKa = 11.84MVKK81 pKa = 9.45GRR83 pKa = 11.84KK84 pKa = 8.69FLSHH88 pKa = 6.98

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7388

0

7388

3644630

50

4468

493.3

54.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.622 ± 0.023

1.066 ± 0.009

5.722 ± 0.021

6.035 ± 0.027

3.371 ± 0.018

7.172 ± 0.027

2.406 ± 0.012

4.751 ± 0.021

4.604 ± 0.026

8.991 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.01

3.182 ± 0.015

6.572 ± 0.034

3.828 ± 0.017

6.311 ± 0.026

9.261 ± 0.041

5.918 ± 0.016

6.034 ± 0.018

1.444 ± 0.01

2.476 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski