Wenling tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGU4|A0A1L3KGU4_9VIRU CCHC-type domain-containing protein OS=Wenling tombus-like virus 1 OX=1923543 PE=4 SV=1
MM1 pKa = 6.54TQPRR5 pKa = 11.84PSHH8 pKa = 5.97TNNSNKK14 pKa = 8.28TMRR17 pKa = 11.84CYY19 pKa = 10.17RR20 pKa = 11.84CNEE23 pKa = 3.98EE24 pKa = 3.51GHH26 pKa = 6.43IARR29 pKa = 11.84DD30 pKa = 3.64CVPQRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GGRR41 pKa = 11.84GSGQAQRR48 pKa = 11.84AVRR51 pKa = 11.84ASDD54 pKa = 3.03EE55 pKa = 4.35RR56 pKa = 11.84SRR58 pKa = 11.84HH59 pKa = 3.45VHH61 pKa = 6.02SCVKK65 pKa = 10.49CCGLYY70 pKa = 10.41SHH72 pKa = 7.11IHH74 pKa = 6.43PYY76 pKa = 9.25NHH78 pKa = 7.16EE79 pKa = 4.05DD80 pKa = 3.41HH81 pKa = 6.55EE82 pKa = 4.3QRR84 pKa = 11.84PYY86 pKa = 9.28QCPYY90 pKa = 8.01PTCEE94 pKa = 3.96WYY96 pKa = 9.96IGSDD100 pKa = 2.94TAEE103 pKa = 4.26EE104 pKa = 4.62YY105 pKa = 8.17RR106 pKa = 11.84TKK108 pKa = 10.69PQMLEE113 pKa = 4.34PIPTPTPTAPPTPEE127 pKa = 4.1PVDD130 pKa = 3.75PLVGPPIDD138 pKa = 4.64EE139 pKa = 4.52EE140 pKa = 4.32PADD143 pKa = 3.81EE144 pKa = 4.22STPKK148 pKa = 10.67GFSSFNDD155 pKa = 3.66LKK157 pKa = 11.23DD158 pKa = 3.25HH159 pKa = 6.97LYY161 pKa = 10.7AYY163 pKa = 7.69LTKK166 pKa = 10.56QSAWTEE172 pKa = 3.73RR173 pKa = 11.84TVLNRR178 pKa = 11.84RR179 pKa = 11.84SMVQRR184 pKa = 11.84AEE186 pKa = 3.62TWLVSKK192 pKa = 10.62KK193 pKa = 10.62VEE195 pKa = 4.22SEE197 pKa = 4.09VLRR200 pKa = 11.84TQVIQAVIDD209 pKa = 3.79TVDD212 pKa = 2.81IMNPLEE218 pKa = 4.17VAVTSAAQNVEE229 pKa = 3.24WRR231 pKa = 11.84NQHH234 pKa = 5.18IAANRR239 pKa = 11.84AAAGWLYY246 pKa = 10.35QPPAVGWSLFTKK258 pKa = 10.17FLIITLLFMTSIIAVCLIGPGVVANSIASLAGHH291 pKa = 7.0AVAKK295 pKa = 8.87TVSINISFLLANAAIMAWRR314 pKa = 11.84GRR316 pKa = 11.84EE317 pKa = 3.89RR318 pKa = 11.84APHH321 pKa = 5.11EE322 pKa = 4.47TGNSAVFTSGNMGRR336 pKa = 4.34

Molecular weight: 37.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGU4|A0A1L3KGU4_9VIRU CCHC-type domain-containing protein OS=Wenling tombus-like virus 1 OX=1923543 PE=4 SV=1
MM1 pKa = 7.46WNSIPGCYY9 pKa = 9.98NPFVHH14 pKa = 6.66SNCVRR19 pKa = 11.84NEE21 pKa = 3.76YY22 pKa = 10.61VAMRR26 pKa = 11.84DD27 pKa = 3.19RR28 pKa = 11.84VVGKK32 pKa = 10.51VPVPTEE38 pKa = 3.53RR39 pKa = 11.84GVRR42 pKa = 11.84LLRR45 pKa = 11.84RR46 pKa = 11.84YY47 pKa = 9.18ARR49 pKa = 11.84KK50 pKa = 9.12LVKK53 pKa = 10.34RR54 pKa = 11.84LGKK57 pKa = 8.8TDD59 pKa = 4.15EE60 pKa = 3.9ISYY63 pKa = 10.29DD64 pKa = 3.57QVIDD68 pKa = 3.95HH69 pKa = 5.07YY70 pKa = 10.85HH71 pKa = 4.76GRR73 pKa = 11.84KK74 pKa = 8.26RR75 pKa = 11.84KK76 pKa = 9.36RR77 pKa = 11.84YY78 pKa = 7.77EE79 pKa = 3.59AAKK82 pKa = 10.24EE83 pKa = 4.08SLGRR87 pKa = 11.84LALTKK92 pKa = 10.33NDD94 pKa = 4.24SIIKK98 pKa = 10.49AFVKK102 pKa = 10.6CEE104 pKa = 3.85KK105 pKa = 10.3FNPADD110 pKa = 3.67KK111 pKa = 10.98VNPAPRR117 pKa = 11.84MIQARR122 pKa = 11.84NARR125 pKa = 11.84YY126 pKa = 9.28NLSIARR132 pKa = 11.84WLRR135 pKa = 11.84PIEE138 pKa = 3.83HH139 pKa = 7.31AIYY142 pKa = 10.24RR143 pKa = 11.84LKK145 pKa = 11.01SKK147 pKa = 9.52LTGLPIVGKK156 pKa = 10.24GRR158 pKa = 11.84SLNEE162 pKa = 3.75RR163 pKa = 11.84AEE165 pKa = 4.1LLAKK169 pKa = 10.02KK170 pKa = 10.23FNYY173 pKa = 9.13FKK175 pKa = 11.3NPVVYY180 pKa = 10.7SLDD183 pKa = 3.29ASRR186 pKa = 11.84WDD188 pKa = 3.29QHH190 pKa = 7.95CDD192 pKa = 2.87IKK194 pKa = 11.15LLEE197 pKa = 4.18VEE199 pKa = 3.97HH200 pKa = 6.57WIYY203 pKa = 11.08RR204 pKa = 11.84AMNGSSEE211 pKa = 4.13FATLLQQQLYY221 pKa = 8.59NKK223 pKa = 9.65CFTEE227 pKa = 4.11HH228 pKa = 6.61GIAYY232 pKa = 6.12KK233 pKa = 10.43TKK235 pKa = 8.12GKK237 pKa = 10.48RR238 pKa = 11.84MSGDD242 pKa = 3.08VNTALGNCLLAILLAYY258 pKa = 9.71VVLVDD263 pKa = 3.95FLKK266 pKa = 11.06LKK268 pKa = 8.86EE269 pKa = 4.45TEE271 pKa = 4.84FEE273 pKa = 4.42LLDD276 pKa = 5.14DD277 pKa = 5.25GDD279 pKa = 4.37DD280 pKa = 3.64LLVIVEE286 pKa = 4.39QKK288 pKa = 10.72DD289 pKa = 3.39EE290 pKa = 4.17HH291 pKa = 7.42RR292 pKa = 11.84LEE294 pKa = 4.9GIKK297 pKa = 9.75EE298 pKa = 3.95AYY300 pKa = 10.38LEE302 pKa = 4.23VGHH305 pKa = 6.54EE306 pKa = 3.94IKK308 pKa = 10.85LEE310 pKa = 3.8NRR312 pKa = 11.84ATVLEE317 pKa = 4.14DD318 pKa = 4.52VEE320 pKa = 4.78WCQHH324 pKa = 5.75RR325 pKa = 11.84PVHH328 pKa = 5.88TPNGWRR334 pKa = 11.84FVPNWKK340 pKa = 9.77KK341 pKa = 10.52VLSSTTCDD349 pKa = 3.19SKK351 pKa = 11.35HH352 pKa = 5.41WMHH355 pKa = 6.68EE356 pKa = 3.76NLRR359 pKa = 11.84PSLGHH364 pKa = 6.27TMGKK368 pKa = 9.61CLLSMYY374 pKa = 9.92TGIPVLQEE382 pKa = 3.54YY383 pKa = 10.37CQFLIQQGNKK393 pKa = 9.19DD394 pKa = 3.82AKK396 pKa = 10.18CLKK399 pKa = 10.16DD400 pKa = 3.5HH401 pKa = 6.5YY402 pKa = 11.4LMDD405 pKa = 4.11RR406 pKa = 11.84AYY408 pKa = 9.06TNGGATTDD416 pKa = 3.64TTPVDD421 pKa = 3.45PEE423 pKa = 4.2TRR425 pKa = 11.84LSFQRR430 pKa = 11.84AFGMTVQEE438 pKa = 4.1QLDD441 pKa = 3.65IEE443 pKa = 4.4TRR445 pKa = 11.84IRR447 pKa = 11.84GLTLPTTTLRR457 pKa = 11.84ITASEE462 pKa = 4.19VLPGWKK468 pKa = 8.67WEE470 pKa = 4.12YY471 pKa = 11.18YY472 pKa = 9.57PGTEE476 pKa = 4.58AGLGG480 pKa = 3.55

Molecular weight: 55.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

816

336

480

408.0

46.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.475 ± 1.214

2.451 ± 0.135

4.289 ± 0.602

6.618 ± 0.218

2.451 ± 0.218

5.882 ± 0.311

3.309 ± 0.156

4.902 ± 0.27

5.637 ± 1.577

8.824 ± 1.878

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.104

4.657 ± 0.062

5.637 ± 1.245

3.554 ± 0.363

7.475 ± 0.156

5.392 ± 1.038

6.495 ± 0.384

6.863 ± 0.166

2.083 ± 0.0

3.799 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski